PMID- 33814346
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220924
IS  - 2531-1387 (Electronic)
IS  - 2531-1379 (Print)
IS  - 2531-1379 (Linking)
VI  - 44
IP  - 3
DP  - 2022 Jul-Sep
TI  - Platelet antibodies identification: comparison between two laboratory tests.
PG  - 365-368
LID - S2531-1379(21)00028-6 [pii]
LID - 10.1016/j.htct.2020.12.008 [doi]
AB  - INTRODUCTION: Platelet antibody identification is indispensable for diagnosing 
      the human platelet antigen (HPA) or human leukocyte antigen (HLA) immunization, 
      mostly because it can restrict the compatibility and results of transfusions. 
      Correct detection of these antibodies is of utmost importance for the diagnosis 
      and treatment. METHOD: We present 16 platelet alloimmunization results, comparing 
      two tests with different technologies: the MAIPA (monoclonal antibody 
      immobilization of platelet antigens), as a reference technique, and a bead-based 
      assay, the Pak-Lx. RESULTS: Eleven samples (68.75%) showed agreement in both 
      techniques. Two tests were false negatives in the Pak-Lx: a pan-reactivity in 
      GPIIbIIIa and an anti-HPA-9b. On the other hand, the Pak-Lx was more sensitive to 
      detect a decreasing anti-HPA-5b. The Pak-Lx found an anti-HPA-2b positive, but 
      with a low median fluorescent intensity (MFI), suggesting a false-positive 
      result. Moreover, in one case, the MAIPA was negative for a positive Pak-Lx HLA. 
      CONCLUSION: Antibody platelet diagnosis can sometimes be challenging. The methods 
      seemed similar, the Pak-Lx being faster and simpler than the MAIPA, and they can 
      be complementary to solve clinical issues.
CI  - Copyright (c) 2021 Associacao Brasileira de Hematologia, Hemoterapia e Terapia 
      Celular. Published by Elsevier Espana, S.L.U. All rights reserved.
FAU - Dutra, Valeria de Freitas
AU  - Dutra VF
AD  - Hospital Israelita Albert Einstein, Sao Paulo, SP, Brazil. Electronic address: 
      valeria.dutra@einstein.br.
FAU - Costa, Thiago Henrique
AU  - Costa TH
AD  - Hospital Israelita Albert Einstein, Sao Paulo, SP, Brazil.
FAU - Santos, Leandro Dinalli
AU  - Santos LD
AD  - Hospital Israelita Albert Einstein, Sao Paulo, SP, Brazil.
FAU - Sirianni, Marilia Fernandes Mascarenhas
AU  - Sirianni MFM
AD  - Hospital Israelita Albert Einstein, Sao Paulo, SP, Brazil.
FAU - Aravechia, Maria Giselda
AU  - Aravechia MG
AD  - Hospital Israelita Albert Einstein, Sao Paulo, SP, Brazil.
FAU - Kutner, Jose Mauro
AU  - Kutner JM
AD  - Hospital Israelita Albert Einstein, Sao Paulo, SP, Brazil.
FAU - Bub, Carolina Bonet
AU  - Bub CB
AD  - Hospital Israelita Albert Einstein, Sao Paulo, SP, Brazil.
LA  - eng
PT  - Journal Article
DEP - 20210306
PL  - Brazil
TA  - Hematol Transfus Cell Ther
JT  - Hematology, transfusion and cell therapy
JID - 101725732
PMC - PMC9477764
OTO - NOTNLM
OT  - Antibodies
OT  - HLA
OT  - HPA
OT  - Platelets
EDAT- 2021/04/06 06:00
MHDA- 2021/04/06 06:01
PMCR- 2021/03/06
CRDT- 2021/04/05 05:44
PHST- 2020/10/24 00:00 [received]
PHST- 2020/11/30 00:00 [revised]
PHST- 2020/12/15 00:00 [accepted]
PHST- 2021/04/06 06:00 [pubmed]
PHST- 2021/04/06 06:01 [medline]
PHST- 2021/04/05 05:44 [entrez]
PHST- 2021/03/06 00:00 [pmc-release]
AID - S2531-1379(21)00028-6 [pii]
AID - 10.1016/j.htct.2020.12.008 [doi]
PST - ppublish
SO  - Hematol Transfus Cell Ther. 2022 Jul-Sep;44(3):365-368. doi: 
      10.1016/j.htct.2020.12.008. Epub 2021 Mar 6.