PMID- 33815140
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210406
IS  - 1664-042X (Print)
IS  - 1664-042X (Electronic)
IS  - 1664-042X (Linking)
VI  - 12
DP  - 2021
TI  - Melatonin-Pretreated Mesenchymal Stem Cells Improved Cognition in a Diabetic 
      Murine Model.
PG  - 628107
LID - 10.3389/fphys.2021.628107 [doi]
LID - 628107
AB  - Diabetes mellitus (DM) is a multisystem endocrine disorder affecting the brain. 
      Mesenchymal stem cells (MSCs) pretreated with Melatonin have been shown to 
      increase the potency of MSCs. This work aimed to compare Melatonin, stem cells, 
      and stem cells pretreated with Melatonin on the cognitive functions and markers 
      of synaptic plasticity in an animal model of type I diabetes mellitus (TIDM). 
      Thirty-six rats represented the animal model; six rats for isolation of MSCs and 
      30 rats were divided into five groups: control, TIDM, TIDM + Melatonin, TIDM + 
      Stem cells, and TIDM + Stem ex vivo Melatonin. Functional assessment was 
      performed with Y-maze, forced swimming test and novel object recognition. 
      Histological and biochemical evaluation of hippocampal Neuroligin 1, Sortilin, 
      Brain-Derived Neurotrophic Factor (BDNF), inducible nitric oxide synthase (iNOS), 
      toll-like receptor 2 (TLR2), Tumor necrosis factor-alpha (TNF-alpha), and Growth 
      Associated Protein 43 (GAP43). The TIDM group showed a significant decrease of 
      hippocampal Neuroligin, Sortilin, and BDNF and a significant increase in iNOS, 
      TNF-alpha, TLR2, and GAP43. Melatonin or stem cells groups showed improvement 
      compared to the diabetic group but not compared to the control group. TIDM + Stem 
      ex vivo Melatonin group showed a significant improvement, and some values were 
      restored to normal. Ex vivo melatonin-treated stem cells had improved spatial 
      working and object recognition memory and depression, with positive effects on 
      glucose homeostasis, inflammatory markers levels and synaptic plasticity markers 
      expression.
CI  - Copyright (c) 2021 Amin, Sharawy, El Tablawy, Elberry, Youssef, Abdelhady, Rashed 
      and Hassan.
FAU - Amin, Shaimaa Nasr
AU  - Amin SN
AD  - Department of Basic Medical Sciences, Faculty of Medicine, The Hashemite 
      University, Zarqa, Jordan.
AD  - Department of Medical Physiology, Faculty of Medicine, Cairo University, Cairo, 
      Egypt.
FAU - Sharawy, Nivin
AU  - Sharawy N
AD  - Department of Medical Physiology, Faculty of Medicine, Cairo University, Cairo, 
      Egypt.
FAU - El Tablawy, Nashwa
AU  - El Tablawy N
AD  - Department of Medical Physiology, Faculty of Medicine, Cairo University, Cairo, 
      Egypt.
FAU - Elberry, Dalia Azmy
AU  - Elberry DA
AD  - Department of Medical Physiology, Faculty of Medicine, Cairo University, Cairo, 
      Egypt.
FAU - Youssef, Mira Farouk
AU  - Youssef MF
AD  - Department of Histology, Faculty of Medicine, Cairo University, Cairo, Egypt.
FAU - Abdelhady, Ebtehal Gamal
AU  - Abdelhady EG
AD  - Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, 
      Beni-Suef University, Beni Suef, Egypt.
FAU - Rashed, Laila Ahmed
AU  - Rashed LA
AD  - Department of Biochemistry, Faculty of Medicine, Cairo University, Cairo, Egypt.
FAU - Hassan, Sherif Sabry
AU  - Hassan SS
AD  - Department of Medical Education, School of Medicine, California University of 
      Science and Medicine, San Bernardino, CA, United States.
AD  - Department of Anatomy, Faculty of Medicine, Cairo University, Cairo, Egypt.
LA  - eng
PT  - Journal Article
DEP - 20210318
PL  - Switzerland
TA  - Front Physiol
JT  - Frontiers in physiology
JID - 101549006
PMC - PMC8012759
OTO - NOTNLM
OT  - cognition
OT  - diabetes
OT  - melatonin
OT  - stem cells
OT  - synaptic plasticity
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2021/04/06 06:00
MHDA- 2021/04/06 06:01
PMCR- 2021/03/18
CRDT- 2021/04/05 05:56
PHST- 2020/11/16 00:00 [received]
PHST- 2021/02/11 00:00 [accepted]
PHST- 2021/04/05 05:56 [entrez]
PHST- 2021/04/06 06:00 [pubmed]
PHST- 2021/04/06 06:01 [medline]
PHST- 2021/03/18 00:00 [pmc-release]
AID - 10.3389/fphys.2021.628107 [doi]
PST - epublish
SO  - Front Physiol. 2021 Mar 18;12:628107. doi: 10.3389/fphys.2021.628107. eCollection 
      2021.