PMID- 33815685
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210406
IS  - 1993-0771 (Print)
IS  - 1993-078X (Electronic)
IS  - 1993-0771 (Linking)
VI  - 15
IP  - 1
DP  - 2021
TI  - Chromosomal mapping of repetitive DNA in Melipona seminigra merrillae Cockerell, 
      1919 (Hymenoptera, Apidae, Meliponini).
PG  - 77-87
LID - 10.3897/CompCytogen.v15i1.56430 [doi]
AB  - Melipona Illiger, 1806 is represented by 74 known species of stingless bees, 
      distributed throughout the Neotropical region. Cytogenetically it is the most 
      studied stingless bee genus of the tribe Meliponini. Member species are divided 
      in two groups based on the volume of heterochromatin. This study aim was to 
      analyze the composition and organization of chromatin of the stingless bee 
      subspecies Melipona seminigra merrillae Cockerell, 1919 using classical and 
      molecular cytogenetic techniques, so contributing to a better understanding of 
      the processes of chromosomal changes within the genus. We confirm that M. 
      seminigra merrillae has a chromosome number of 2n = 22 and n = 11, results that 
      differ from those reported for the genus in the absence of B chromosomes. The 
      heterochromatic pattern revealed a karyotype composed of chromosomes with a high 
      heterochromatin content, which makes it difficult to visualize the centromere. 
      Silver nitrate impregnation (Ag-NOR) showed transcriptionally active sites on the 
      second chromosomal pair. Staining of base-specific fluorophores DAPI-CMA(3) 
      indicated a homogeneous distribution of intensely DAPI-stained heterochromatin, 
      while CMA(3) markings appeared on those terminal portions of the chromosomes 
      corresponding to euchromatin. Similar to Ag-NOR, fluorescence in situ 
      hybridization (FISH) with 18S ribosomal DNA probe revealed distinct signals on 
      the second pair of chromosomes. Microsatellite mapping (GA)(15) showed markings 
      distributed in euchromatic regions, while mapping with (CA)(15) showed marking 
      patterns in heterochromatic regions, together with a fully marked chromosome 
      pair. Microsatellite hybridization, both in heterochromatic and euchromatic 
      regions, may be related to the activity of transposable elements. These are 
      capable of forming new microsatellites that can be dispersed and amplified in 
      different regions of the genome, demonstrating that repetitive sequences can 
      evolve rapidly, thus resulting in within-genus diversification.
CI  - Ingrid Candido de Oliveira Barbosa, Carlos Henrique Schneider, Leonardo Gusso 
      Goll, Eliana Feldberg, Gislene Almeida Carvalho-Zilse.
FAU - Barbosa, Ingrid Candido de Oliveira
AU  - Barbosa ICO
AD  - Grupo de Pesquisas em Abelhas, Programa de Pos-Graduacao em Genetica, Conservacao 
      e Biologia Evolutiva, Instituto Nacional de Pesquisas da Amazonia, Av. Andre 
      Araujo 2936, Petropolis, 69067-375, Manaus, Brazil.
FAU - Schneider, Carlos Henrique
AU  - Schneider CH
AD  - Laboratorio de Pesquisa em Ciencias Medicas, Universidade Federal da Integracao 
      Latino Americana, Av. Silvio Americo Sasdelli 1842, Itaipu A, 85866-000, Foz do 
      Iguacu, Brazil.
FAU - Goll, Leonardo Gusso
AU  - Goll LG
AD  - Instituto de Natureza e Cultura - INC, R. Primeiro de Maio s/n, Colonia, 
      69630-000, Benjamin Constant, Brazil.
FAU - Feldberg, Eliana
AU  - Feldberg E
AUID- ORCID: 0000-0002-2962-4541
AD  - Laboratorio de Genetica Animal, Programa de Pos-Graduacao em Genetica, 
      Conservacao e Biologia Evolutiva, Instituto Nacional de Pesquisas da Amazonia, 
      Av. Andre Araujo 2936, Petropolis, 69067-375, Manaus, Brazil.
FAU - Carvalho-Zilse, Gislene Almeida
AU  - Carvalho-Zilse GA
AD  - Grupo de Pesquisas em Abelhas, Coordenacao de Biodiversidade, Instituto Nacional 
      de Pesquisas da Amazonia, Av. Andre Araujo 2936, Petropolis, 69067-375, Manaus, 
      Brazil.
LA  - eng
PT  - Journal Article
DEP - 20210319
PL  - Bulgaria
TA  - Comp Cytogenet
JT  - Comparative cytogenetics
JID - 101503807
PMC - PMC7997856
OTO - NOTNLM
OT  - Cytogenetics
OT  - fluorescence in situ hybridization (FISH)
OT  - heterochromatin
OT  - stingless bee
EDAT- 2021/04/06 06:00
MHDA- 2021/04/06 06:01
PMCR- 2021/03/19
CRDT- 2021/04/05 06:00
PHST- 2020/07/13 00:00 [received]
PHST- 2021/02/03 00:00 [accepted]
PHST- 2021/04/05 06:00 [entrez]
PHST- 2021/04/06 06:00 [pubmed]
PHST- 2021/04/06 06:01 [medline]
PHST- 2021/03/19 00:00 [pmc-release]
AID - 56430 [pii]
AID - 10.3897/CompCytogen.v15i1.56430 [doi]
PST - epublish
SO  - Comp Cytogenet. 2021 Mar 19;15(1):77-87. doi: 10.3897/CompCytogen.v15i1.56430. 
      eCollection 2021.