PMID- 33816171
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220421
IS  - 2223-4292 (Print)
IS  - 2223-4306 (Electronic)
IS  - 2223-4306 (Linking)
VI  - 11
IP  - 4
DP  - 2021 Apr
TI  - Feasibility study for ex vivo fluorescence confocal microscopy (FCM) on 
      diagnostic prostate biopsies.
PG  - 1322-1332
LID - 10.21037/qims-20-895 [doi]
AB  - BACKGROUND: Fluorescence confocal microscopy (FCM) is a novel micro-imaging 
      technique providing optical sections of examined tissue. The method has been well 
      established for the diagnosis of tumours in dermatological specimens. Preliminary 
      results found good feasibility when this technique was used to examine prostate 
      cancer (PCa) specimens. METHODS: We report on the application of FCM in magnet 
      resonance imaging (MRI)-fused prostate biopsies (10 patients, total number of 
      biopsy specimens: n=121) and compare the results to conventional histology. 
      RESULTS: Specific structures of the prostatic tissue were very well represented 
      in the FCM images comparable to conventional histology. Prostate carcinoma was 
      diagnosed with good sensitivity (79/68%) and high specificity (100%) by two 
      pathologists with substantial/almost perfect levels of agreement with the results 
      of conventional histology (kappa 0.79/0.86). Depending on the quality of the 
      scans, malignant lesions of 1.8 mm and more in diameter were reliably diagnosed. 
      Smaller lesions were rated as suspect for malignancy, but could not be 
      consistently differentiated from reactive changes. Optimal image qualities were 
      achieved in focus depths of up to 50 microm, whereas deeper scans led to insufficient 
      representation of cytological features. Pre-treatment with acridine orange (AO) 
      did not alter immunoreactivity of the tissue or its feasibility for fluorescence 
      in situ hybridization (FISH) analyses and adequate amounts of DNA could be 
      extracted for further polymerase chain reaction (PCR)-based examinations. 
      CONCLUSIONS: FCM seems to be a promising tool for the timely diagnosis in cases 
      of PCa in patients requiring therapy. In particular, this technique is a 
      material-sparing method that conserves the biopsies as unfixed material for 
      further analysis such as molecular tumour companion diagnosis.
CI  - 2021 Quantitative Imaging in Medicine and Surgery. All rights reserved.
FAU - Titze, Ulf
AU  - Titze U
AD  - Department of Pathology, Klinikum Lippe GmbH, Detmold, Germany.
FAU - Hansen, Torsten
AU  - Hansen T
AD  - Department of Pathology, Klinikum Lippe GmbH, Detmold, Germany.
FAU - Titze, Barbara
AU  - Titze B
AD  - Department of Pathology, Klinikum Lippe GmbH, Detmold, Germany.
FAU - Schulz, Birte
AU  - Schulz B
AD  - Department of Pathology, Klinikum Lippe GmbH, Detmold, Germany.
FAU - Gunnemann, Alfons
AU  - Gunnemann A
AD  - Department of Urology, Klinikum Lippe GmbH, Detmold, Germany.
FAU - Rocco, Bernardo
AU  - Rocco B
AD  - Department of Urology, University of Modena and Reggio Emilia, Modena, Italy.
FAU - Sievert, Karl-Dietrich
AU  - Sievert KD
AD  - Department of Urology, Klinikum Lippe GmbH, Detmold, Germany.
LA  - eng
PT  - Journal Article
PL  - China
TA  - Quant Imaging Med Surg
JT  - Quantitative imaging in medicine and surgery
JID - 101577942
PMC - PMC7930678
OTO - NOTNLM
OT  - Confocal microscopy
OT  - digital pathology
OT  - prostate biopsies
OT  - prostate cancer (PCa)
COIS- Conflicts of Interest: All authors have completed the ICMJE uniform disclosure 
      form (available at http://dx.doi.org/10.21037/qims-20-895). The authors have no 
      conflicts of interest to declare.
EDAT- 2021/04/06 06:00
MHDA- 2021/04/06 06:01
PMCR- 2021/04/01
CRDT- 2021/04/05 06:08
PHST- 2021/04/05 06:08 [entrez]
PHST- 2021/04/06 06:00 [pubmed]
PHST- 2021/04/06 06:01 [medline]
PHST- 2021/04/01 00:00 [pmc-release]
AID - qims-11-04-1322 [pii]
AID - 10.21037/qims-20-895 [doi]
PST - ppublish
SO  - Quant Imaging Med Surg. 2021 Apr;11(4):1322-1332. doi: 10.21037/qims-20-895.