PMID- 33817193
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210406
IS  - 2391-5412 (Electronic)
IS  - 2391-5412 (Linking)
VI  - 14
DP  - 2019 Jan
TI  - Co-cultured Bone-marrow Derived and Tendon Stem Cells: Novel Seed Cells for Bone 
      Regeneration.
PG  - 568-575
LID - 10.1515/biol-2019-0063 [doi]
AB  - Tendon-bone healing after injury is an unsolved problem. Several types of stem 
      cells are used as seed cells. However, the optimal co-culture ratio of different 
      types of cells suitable for tissue engineering as well as the stimulator for 
      facilitating the differentiation of stem cells in tendon-bone healing is unclear. 
      In this study, the proliferation of both bone marrow-derived stem cells (BMSCs) 
      and tendon stem cells (TSCs) was increased at a 1:1 co-cultured ratio, and 
      proliferation was suppressed by Tenascin C (TNC). TNC treatment can promote 
      osteogenesis or chondrogenesis of both BMSCs and TSCs under a 1:1 co-cultured 
      ratio. In addition, the expression level of Rho-associated kinase (ROCK) 
      increased in the process of TNC-induced osteogenesis and decreased in the process 
      of TNC-induced chondrogenesis. Furthermore, the level of insulin-like growth 
      factor 1 receptor (IGF-1R) and mitogen-activated protein kinase (MEK) was 
      upregulated during the osteogenesis and chondrogenesis of both BMSCs and TSCs 
      after TNC treatment. Although our study was conducted in rats with no direct 
      evaluation of the resulting cells for tendon-bone healing and regeneration, we 
      show that the proliferation of BMSCs and TSCs was enhanced under a 1:1 
      co-cultured ratio. TNC has a significant impact on the proliferation and 
      differentiation of co-cultured BMSCs and TSCs. IGF-IR, ROCK, and MEK may become 
      involved in the process after TNC treatment.
CI  - (c) 2019 Yang Liu et al., published by De Gruyter.
FAU - Liu, Yang
AU  - Liu Y
AD  - Department of Orthopaedics, First Affiliated Hospital, Army Medical University, 
      Chongqing, P.R. China 400038.
FAU - Yuan, Chengsong
AU  - Yuan C
AD  - Department of Orthopaedics, First Affiliated Hospital, Army Medical University, 
      Chongqing, P.R. China 400038.
FAU - Zhou, Mei
AU  - Zhou M
AD  - Department of Orthopaedics, First Affiliated Hospital, Army Medical University, 
      Chongqing, P.R. China 400038.
FAU - Tang, Kanglai
AU  - Tang K
AD  - Department of Orthopaedics, First Affiliated Hospital, Army Military Medical 
      University, Chongqing 400038, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20191231
PL  - Poland
TA  - Open Life Sci
JT  - Open life sciences
JID - 101669614
PMC - PMC7874801
OTO - NOTNLM
OT  - Bone-marrow derived stem cells
OT  - Co-culture
OT  - Tenascin-C
OT  - Tendon stem cells
COIS- Conflict of interest: Authors state no conflict of interest.
EDAT- 2019/12/31 00:00
MHDA- 2019/12/31 00:01
PMCR- 2019/12/31
CRDT- 2021/04/05 06:13
PHST- 2019/03/18 00:00 [received]
PHST- 2019/07/18 00:00 [accepted]
PHST- 2021/04/05 06:13 [entrez]
PHST- 2019/12/31 00:00 [pubmed]
PHST- 2019/12/31 00:01 [medline]
PHST- 2019/12/31 00:00 [pmc-release]
AID - biol-2019-0063 [pii]
AID - 10.1515/biol-2019-0063 [doi]
PST - epublish
SO  - Open Life Sci. 2019 Dec 31;14:568-575. doi: 10.1515/biol-2019-0063. eCollection 
      2019 Jan.