PMID- 33817711
OWN - NLM
STAT- MEDLINE
DCOM- 20211028
LR  - 20220531
IS  - 2066-8279 (Electronic)
IS  - 1220-0522 (Print)
IS  - 1220-0522 (Linking)
VI  - 61
IP  - 3
DP  - 2020 Jul-Sep
TI  - Different patterns of p16INK4a immunohistochemical expression and their 
      biological implications in laryngeal squamous cell carcinoma.
PG  - 697-706
LID - 10.47162/RJME.61.3.08 [doi]
AB  - INTRODUCTION: p16INK4a immunohistochemistry (IHC) is widely used to facilitate 
      the diagnosis of human papillomavirus (HPV)-associated neoplasia, when >/=70% of 
      cells show strong nuclear and cytoplasmic positivity. In this study, we aim to 
      compare partial expression patterns that do not fulfill the above criteria and 
      seek biological implications in laryngeal squamous cell carcinoma (LSCC). 
      MATERIALS AND METHODS: p16INK4a IHC staining was conducted on representative 
      sections of archived tissue from 88 LSCCs. Immunoreactivity was described based 
      on four parameters: intracellular localization of immunostaining, intensity of 
      immunostaining, distribution pattern and percentage of positive cells. RESULTS: 
      Six patterns of p16INK4a immunoexpression were observed and defined as: strong 
      diffuse (strong immunostaining, expression in cytoplasm and nucleus in >70% of 
      tumor cells), weak diffuse (moderate or weak immunostaining, expression in 
      cytoplasm in >70% of tumor cells), marginal (strong cytoplasmic immunostaining, 
      limited to the periphery of tumor islets), strong scattered (strong 
      immunostaining, expression in cytoplasm and nucleus in <50% of tumor cells), weak 
      scattered (moderate or weak immunostaining, expression in cytoplasm in <50% of 
      tumor cells), negative (no expression). The pN stage of the patients was 
      associated with p16INK4a immunoexpression patterns, the marginal pattern was only 
      found in the pN0-Nx stages, while the weak diffuse pattern was more frequently 
      observed in pN2-N3 stages. CONCLUSIONS: Partial immunostaining with 
      architecturally distinct p16INK4a immunoexpression patterns may prove significant 
      in stratifying characteristic clinicopathological subgroups among LSCC. Our 
      observations may support the hypothesis that p16INK4a has different roles in 
      different subcellular locations, with tumorigenic molecular pathways unrelated to 
      HPV infection.
FAU - Lazar, Camelia Sidonia
AU  - Lazar CS
AD  - Discipline of Histology, Department of Morphological Sciences, Iuliu Hatieganu 
      University of Medicine and Pharmacy, Cluj-Napoca, Romania; a_sovrea@yahoo.com.
FAU - Sovrea, Alina Simona
AU  - Sovrea AS
FAU - Georgiu, Carmen
AU  - Georgiu C
FAU - Crisan, Doinita
AU  - Crisan D
FAU - Mirescu, Stefan Claudiu
AU  - Mirescu SC
FAU - Cosgarea, Marcel
AU  - Cosgarea M
LA  - eng
PT  - Journal Article
PL  - Romania
TA  - Rom J Morphol Embryol
JT  - Romanian journal of morphology and embryology = Revue roumaine de morphologie et 
      embryologie
JID - 9112454
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Cyclin-Dependent Kinase Inhibitor p16)
SB  - IM
MH  - Biomarkers, Tumor
MH  - *Carcinoma, Squamous Cell
MH  - Cyclin-Dependent Kinase Inhibitor p16
MH  - Female
MH  - *Head and Neck Neoplasms
MH  - Humans
MH  - Papillomaviridae
MH  - Squamous Cell Carcinoma of Head and Neck
MH  - *Uterine Cervical Neoplasms
PMC - PMC8112783
COIS- The authors declare that they have no conflict of interests.
EDAT- 2021/04/06 06:00
MHDA- 2021/10/29 06:00
PMCR- 2020/07/01
CRDT- 2021/04/05 06:20
PHST- 2021/04/05 06:20 [entrez]
PHST- 2021/04/06 06:00 [pubmed]
PHST- 2021/10/29 06:00 [medline]
PHST- 2020/07/01 00:00 [pmc-release]
AID - 610320697706 [pii]
AID - 10.47162/RJME.61.3.08 [doi]
PST - ppublish
SO  - Rom J Morphol Embryol. 2020 Jul-Sep;61(3):697-706. doi: 10.47162/RJME.61.3.08.