PMID- 33821317
OWN - NLM
STAT- MEDLINE
DCOM- 20211018
LR  - 20220813
IS  - 0942-0940 (Electronic)
IS  - 0001-6268 (Print)
IS  - 0001-6268 (Linking)
VI  - 163
IP  - 8
DP  - 2021 Aug
TI  - Exosomes secreted from sonic hedgehog-modified bone mesenchymal stem cells 
      facilitate the repair of rat spinal cord injuries.
PG  - 2297-2306
LID - 10.1007/s00701-021-04829-9 [doi]
AB  - BACKGROUND: Spinal cord injuries (SCIs) can cause a loss of neurons and 
      associated sensory and motor functionality below the injured site. No approaches 
      to treating SCIs in humans have been developed to date. Exosomes are 
      extracellular vesicles that hold promise as a potential therapeutic modality when 
      treating such injuries. The present study was thus designed to determine whether 
      sonic hedgehog (Shh)-overexpressing bone mesenchymal stem cell (BMSC)-derived 
      exosomes were protective in the context of SCIs. METHODS: Exosomes were extracted 
      from control or Shh lentivirus-transduced BMSCs, yielding respective BMSC-Exo and 
      BMSC-Shh-Exo preparations which were intravenously injected into SCI model rats. 
      Shh expression in spinal cord tissues in these animals was then assessed via 
      immunohistochemical staining, while Basso-Beattie-Bresnahan (BBB) scores were 
      utilized to measure high limb motor function. Neuronal damage and regeneration 
      within the spinal cord were additionally evaluated via terminal deoxynucleotidyl 
      transferase dUTP nick end labeling (TUNEL), Nissl, hematoxylin and eosin, and 
      immunofluorescent staining. RESULTS: Both BMSC-Exo and BMSC-Shh-Exo preparations 
      significantly increased Shh expression in the spinal cord of SCI model rats and 
      improved BBB scores in these treated animals, while also increasing the 
      frequencies of Nissl- and NeuN-positive neurons are reducing the numbers of 
      apoptotic and GFAP-positive neurons. While both treatments yielded some degree of 
      benefit to treated animals relative to untreated controls, BMSC-Shh-Exos were 
      more beneficial than were control BMSC-Exos. CONCLUSIONS: Shh-overexpressing 
      BMSC-derived exosomes represent an effective treatment that can facilitate SCI 
      repair in rats.
CI  - (c) 2021. The Author(s).
FAU - Jia, Yijia
AU  - Jia Y
AD  - Department of Spine Surgery, Guizhou Province Osteological Hospital, 123 Shachong 
      South Street, Nanming District, Guiyang, 550002, Guizhou Province, China.
FAU - Lu, Tingsheng
AU  - Lu T
AD  - Department of Spine Surgery, Guizhou Province Osteological Hospital, 123 Shachong 
      South Street, Nanming District, Guiyang, 550002, Guizhou Province, China.
FAU - Chen, Qiling
AU  - Chen Q
AD  - Department of Spine Surgery, Guizhou Province Osteological Hospital, 123 Shachong 
      South Street, Nanming District, Guiyang, 550002, Guizhou Province, China.
FAU - Pu, Xingwei
AU  - Pu X
AD  - Department of Spine Surgery, Guizhou Province Osteological Hospital, 123 Shachong 
      South Street, Nanming District, Guiyang, 550002, Guizhou Province, China.
FAU - Ji, Linsong
AU  - Ji L
AD  - Department of Spine Surgery, Guizhou Province Osteological Hospital, 123 Shachong 
      South Street, Nanming District, Guiyang, 550002, Guizhou Province, China.
FAU - Yang, Jianwen
AU  - Yang J
AD  - Department of Spine Surgery, Guizhou Province Osteological Hospital, 123 Shachong 
      South Street, Nanming District, Guiyang, 550002, Guizhou Province, China.
FAU - Luo, Chunshan
AU  - Luo C
AD  - Department of Spine Surgery, Guizhou Province Osteological Hospital, 123 Shachong 
      South Street, Nanming District, Guiyang, 550002, Guizhou Province, China. 
      jiayijia2192@126.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210405
PL  - Austria
TA  - Acta Neurochir (Wien)
JT  - Acta neurochirurgica
JID - 0151000
RN  - 0 (Hedgehog Proteins)
SB  - IM
MH  - Animals
MH  - *Exosomes
MH  - Hedgehog Proteins
MH  - Male
MH  - *Mesenchymal Stem Cell Transplantation
MH  - *Mesenchymal Stem Cells
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Recovery of Function
MH  - Spinal Cord
MH  - *Spinal Cord Injuries/therapy
PMC - PMC8270837
OTO - NOTNLM
OT  - BMSCs
OT  - Exosomes
OT  - Sonic hedgehog
OT  - Spinal cord injury
COIS- The authors declare no competing interests.
EDAT- 2021/04/07 06:00
MHDA- 2021/10/21 06:00
PMCR- 2021/04/05
CRDT- 2021/04/06 05:56
PHST- 2021/01/05 00:00 [received]
PHST- 2021/03/23 00:00 [accepted]
PHST- 2021/04/07 06:00 [pubmed]
PHST- 2021/10/21 06:00 [medline]
PHST- 2021/04/06 05:56 [entrez]
PHST- 2021/04/05 00:00 [pmc-release]
AID - 10.1007/s00701-021-04829-9 [pii]
AID - 4829 [pii]
AID - 10.1007/s00701-021-04829-9 [doi]
PST - ppublish
SO  - Acta Neurochir (Wien). 2021 Aug;163(8):2297-2306. doi: 
      10.1007/s00701-021-04829-9. Epub 2021 Apr 5.