PMID- 3382146
OWN - NLM
STAT- MEDLINE
DCOM- 19880719
LR  - 20170214
IS  - 0004-5632 (Print)
IS  - 0004-5632 (Linking)
VI  - 25 ( Pt 2)
DP  - 1988 Mar
TI  - A simple enzymic method for the measurement of magnesium in serum and urine on a 
      centrifugal analyser.
PG  - 162-8
AB  - A method is described based on a reaction that requires magnesium-ATP as a 
      co-factor for the activity of hexokinase (HK), coupled with glucose 6-phosphate 
      dehydrogenase (G6PDH). Glucose and NADP are converted to D-gluconolactone 
      6-phosphate and NADPH, respectively. The rate of increase in absorbance at 340 nm 
      due to the formation of NADPH is proportional to the magnesium concentration in 
      the sample. Magnesium levels in serum and urine measured by the enzymic method 
      compared well with those obtained by atomic absorption spectrometry. The 
      within-batch precisions were 1.4% and 1.5% for the enzymic method and the atomic 
      absorption method, respectively for a quality assurance sample with a magnesium 
      concentration of 2 mmol/L. The enzymic method is accurate (recoveries of added 
      magnesium to serum samples are 101-102%), reproducible (between batch CV 2.8%), 
      and rapid (23 samples may be measured in 10 min). Data on accuracy, precision and 
      correlation for the enzymic and atomic absorption methods are presented.
FAU - Tsang, W M
AU  - Tsang WM
AD  - Department of Chemical Pathology, University College and Middlesex School of 
      Medicine, London, UK.
FAU - Howell, M J
AU  - Howell MJ
FAU - Miller, A L
AU  - Miller AL
LA  - eng
PT  - Journal Article
PL  - England
TA  - Ann Clin Biochem
JT  - Annals of clinical biochemistry
JID - 0324055
RN  - EC 1.1.1.49 (Glucosephosphate Dehydrogenase)
RN  - EC 2.7.1.1 (Hexokinase)
RN  - I38ZP9992A (Magnesium)
SB  - IM
MH  - Autoanalysis
MH  - Glucosephosphate Dehydrogenase
MH  - Hexokinase
MH  - Humans
MH  - Magnesium/*analysis
MH  - Spectrophotometry, Atomic
EDAT- 1988/03/01 00:00
MHDA- 1988/03/01 00:01
CRDT- 1988/03/01 00:00
PHST- 1988/03/01 00:00 [pubmed]
PHST- 1988/03/01 00:01 [medline]
PHST- 1988/03/01 00:00 [entrez]
AID - 10.1177/000456328802500206 [doi]
PST - ppublish
SO  - Ann Clin Biochem. 1988 Mar;25 ( Pt 2):162-8. doi: 10.1177/000456328802500206.