PMID- 33821684
OWN - NLM
STAT- MEDLINE
DCOM- 20211117
LR  - 20211117
IS  - 1555-3892 (Electronic)
IS  - 0963-6897 (Print)
IS  - 0963-6897 (Linking)
VI  - 30
DP  - 2021 Jan-Dec
TI  - LncRNA MNX1-AS1 Contributes to Laryngeal Squamous Cell Carcinoma Growth and 
      Migration by Regulating mir-744-5p/bcl9/beta-Catenin Axis.
PG  - 9636897211005682
LID - 10.1177/09636897211005682 [doi]
LID - 09636897211005682
AB  - Increasing evidence has indicated that long noncoding RNAs (lncRNAs) are involved 
      in the progression of laryngeal squamous cell carcinoma (LSCC). Here, we aimed to 
      disclose the role of MNX1-AS1 in LSCC progression, and explore whether MNX1-AS1 
      participates in LSCC progression via targeting miR-744-5p to active 
      BCL9/beta-catenin signaling. Sixty-five human LSCC tissues and the paracancerous 
      normal tissues were recruited to determine the levels of MNX1-AS1, miR-744-5p and 
      BCL9 using qRT-PCR. The interaction of miR-744-5p and MNX1-AS1/BCL9 was 
      determined by using the RNA immunoprecipitation (RIP) assay and/or luciferase 
      gene reporter assay. Cell viability, in vivo tumor formation, invasion and 
      migration abilities were detected by MTT, Xenograft models and Transwell assays. 
      MNX1-AS1 level was increased significantly in human LSCC tissues as compared with 
      the normal tissues, which showed a positive correlation with BCL9 level while a 
      negative correlation with miR-744-5p level. High level of MNX1-AS1 predicted a 
      poor prognosis and an advanced clinical process in LSCC patients. miR-744-5p 
      targeted upregulation weakened the luciferase activity of MNX1-AS1 and /BCL9, and 
      downregulated their expression levels-wt, while showed no effect when the binding 
      sites were mutated. Knockdown of MNX1-AS1 markedly weakened cell viability, 
      migration, and invasion abilities, while BCL9 overexpression abolished these 
      tendencies. In addition, MNX1-AS1 downregulation induced decreases in tumor 
      volumes and weights in vivo, accompanied by reductions in BCL9, Ki-67 and 
      beta-catenin expression and an increase in miR-744-5p expression. Collectively, this 
      study reveals that MNX1-AS1 contributes to cell growth and migration by 
      regulating miR-744-5p/BCL9/beta-catenin axis in LSCC.
FAU - Ma, Bingliang
AU  - Ma B
AD  - Department of Otolaryngology, the First Affiliated Hospital, Huzhou University, 
      the First People's Hospital of Huzhou, Huzhou City, Zhejiang Province, China.
FAU - Ren, Gang
AU  - Ren G
AUID- ORCID: 0000-0002-0107-0524
AD  - Department of Otolaryngology, the First Affiliated Hospital, Huzhou University, 
      the First People's Hospital of Huzhou, Huzhou City, Zhejiang Province, China.
FAU - Xu, Jue
AU  - Xu J
AD  - Department of Otolaryngology, the First Affiliated Hospital, Huzhou University, 
      the First People's Hospital of Huzhou, Huzhou City, Zhejiang Province, China.
FAU - Yin, Chenyi
AU  - Yin C
AD  - Department of Otolaryngology, the First Affiliated Hospital, Huzhou University, 
      the First People's Hospital of Huzhou, Huzhou City, Zhejiang Province, China.
FAU - Shi, Yuye
AU  - Shi Y
AD  - Department of Surgical Anesthesiology, the First Affiliated Hospital, Huzhou 
      University, the First People's Hospital of Huzhou, Huzhou City, Zhejiang 
      Province, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cell Transplant
JT  - Cell transplantation
JID - 9208854
RN  - 0 (MIRN744 microRNA, mouse)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (beta Catenin)
SB  - IM
MH  - Animals
MH  - Carcinoma, Squamous Cell/*genetics/pathology
MH  - Cell Line, Tumor
MH  - Cell Movement
MH  - Cell Proliferation
MH  - Disease Models, Animal
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics/pathology
MH  - Male
MH  - Mice
MH  - Mice, Nude
MH  - MicroRNAs/*metabolism
MH  - RNA, Long Noncoding/*metabolism
MH  - Up-Regulation
MH  - beta Catenin/*metabolism
PMC - PMC8033468
OTO - NOTNLM
OT  - BCL9
OT  - MNX1-AS1
OT  - cell growth
OT  - laryngeal squamous cell carcinoma
OT  - miR-744-5p
COIS- Declaration of Conflicting Interests: The author(s) declared no potential 
      conflicts of interest with respect to the research, authorship, and/or 
      publication of this article.
EDAT- 2021/04/07 06:00
MHDA- 2021/11/18 06:00
PMCR- 2021/04/06
CRDT- 2021/04/06 12:18
PHST- 2021/04/06 12:18 [entrez]
PHST- 2021/04/07 06:00 [pubmed]
PHST- 2021/11/18 06:00 [medline]
PHST- 2021/04/06 00:00 [pmc-release]
AID - 10.1177_09636897211005682 [pii]
AID - 10.1177/09636897211005682 [doi]
PST - ppublish
SO  - Cell Transplant. 2021 Jan-Dec;30:9636897211005682. doi: 
      10.1177/09636897211005682.