PMID- 33822091
OWN - NLM
STAT- MEDLINE
DCOM- 20210707
LR  - 20210707
IS  - 1938-291X (Electronic)
IS  - 0022-0493 (Linking)
VI  - 114
IP  - 3
DP  - 2021 Jun 11
TI  - Optimization of a Diet for the Greater Wax Moth (Lepidoptera: Pyralidae) Using 
      Full Factorial and Mixture Design.
PG  - 1091-1103
LID - 10.1093/jee/toab039 [doi]
AB  - Diet optimization is an important process to increase the efficiency of rearing 
      insects and can be used to develop high-quality insects with specific fitness and 
      life-history traits. Galleria mellonella (L.), the greater wax moth, is widely 
      used in research, microbiology assays, as pet food, and host for biological 
      control agents. Although artificial diets for G. mellonella have been researched 
      and optimized for decades, preliminary tests indicated that the predominantly 
      utilized G. mellonella diet could be improved to yield larger larvae with a short 
      development time. We used a design of experiments (DOE) approach that 
      incorporated multiple full factorial designs and a final mixture design to test 
      the qualitative and quantitative effects of ingredients and their interactions on 
      larval mass and survival. Analysis of 17 ingredient variations in 35 diet 
      formulations yielded an optimized diet that supported high survival and 2.4-fold 
      greater larval body mass than the standard rearing diet. This study demonstrates 
      the importance and efficiency of statistical DOE in guiding the optimization of 
      insect diets to improve traits that represent the quality and fitness of the 
      reared insects.
CI  - Published by Oxford University Press on behalf of Entomological Society of 
      America 2021.
FAU - Hickin, Mauri
AU  - Hickin M
AUID- ORCID: 0000-0002-6735-9347
AD  - Department of Entomology and Plant Pathology, North Carolina State University, 
      Campus Box 7613, Raleigh, NC 27695-7613, USA.
AD  - Otis Laboratory, USDA APHIS PPQ S&T, 1398 West Truck Road, Buzzards Bay, MA 
      02542, USA.
FAU - Nadel, Hannah
AU  - Nadel H
AD  - Otis Laboratory, USDA APHIS PPQ S&T, 1398 West Truck Road, Buzzards Bay, MA 
      02542, USA.
FAU - Schal, Coby
AU  - Schal C
AUID- ORCID: 0000-0001-7195-6358
AD  - Department of Entomology and Plant Pathology, North Carolina State University, 
      Campus Box 7613, Raleigh, NC 27695-7613, USA.
FAU - Cohen, Allen C
AU  - Cohen AC
AD  - Department of Entomology and Plant Pathology, North Carolina State University, 
      Campus Box 7613, Raleigh, NC 27695-7613, USA.
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - England
TA  - J Econ Entomol
JT  - Journal of economic entomology
JID - 2985127R
SB  - IM
MH  - Animals
MH  - Diet
MH  - Larva
MH  - *Lepidoptera
MH  - *Moths
OTO - NOTNLM
OT  - design of experiments
OT  - diet optimization
OT  - rearing
EDAT- 2021/04/07 06:00
MHDA- 2021/07/08 06:00
CRDT- 2021/04/06 12:35
PHST- 2020/10/21 00:00 [received]
PHST- 2021/04/07 06:00 [pubmed]
PHST- 2021/07/08 06:00 [medline]
PHST- 2021/04/06 12:35 [entrez]
AID - 6208903 [pii]
AID - 10.1093/jee/toab039 [doi]
PST - ppublish
SO  - J Econ Entomol. 2021 Jun 11;114(3):1091-1103. doi: 10.1093/jee/toab039.