PMID- 33823021
OWN - NLM
STAT- MEDLINE
DCOM- 20210820
LR  - 20240104
IS  - 1532-298X (Electronic)
IS  - 1040-4651 (Print)
IS  - 1040-4651 (Linking)
VI  - 33
IP  - 4
DP  - 2021 May 31
TI  - CRISPR screens in plants: approaches, guidelines, and future prospects.
PG  - 794-813
LID - 10.1093/plcell/koab099 [doi]
AB  - Clustered regularly interspaced short palindromic repeat (CRISPR)-associated 
      systems have revolutionized genome engineering by facilitating a wide range of 
      targeted DNA perturbations. These systems have resulted in the development of 
      powerful new screens to test gene functions at the genomic scale. While there is 
      tremendous potential to map and interrogate gene regulatory networks at 
      unprecedented speed and scale using CRISPR screens, their implementation in 
      plants remains in its infancy. Here we discuss the general concepts, tools, and 
      workflows for establishing CRISPR screens in plants and analyze the handful of 
      recent reports describing the use of this strategy to generate mutant knockout 
      collections or to diversify DNA sequences. In addition, we provide insight into 
      how to design CRISPR knockout screens in plants given the current challenges and 
      limitations and examine multiple design options. Finally, we discuss the unique 
      multiplexing capabilities of CRISPR screens to investigate redundant gene 
      functions in highly duplicated plant genomes. Combinatorial mutant screens have 
      the potential to routinely generate higher-order mutant collections and 
      facilitate the characterization of gene networks. By integrating this approach 
      with the numerous genomic profiles that have been generated over the past two 
      decades, the implementation of CRISPR screens offers new opportunities to analyze 
      plant genomes at deeper resolution and will lead to great advances in functional 
      and synthetic biology.
CI  - � American Society of Plant Biologists 2021. All rights reserved. For 
      permissions, please email: journals.permissions@oup.com.
FAU - Gaillochet, Christophe
AU  - Gaillochet C
AD  - Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent 
      9052, Belgium.
AD  - VIB Center for Plant Systems Biology, Ghent 9052, Belgium.
FAU - Develtere, Ward
AU  - Develtere W
AD  - Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent 
      9052, Belgium.
AD  - VIB Center for Plant Systems Biology, Ghent 9052, Belgium.
FAU - Jacobs, Thomas B
AU  - Jacobs TB
AD  - Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent 
      9052, Belgium.
AD  - VIB Center for Plant Systems Biology, Ghent 9052, Belgium.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - England
TA  - Plant Cell
JT  - The Plant cell
JID - 9208688
RN  - 0 (RNA, Guide, CRISPR-Cas Systems)
SB  - IM
MH  - CRISPR-Cas Systems
MH  - *Clustered Regularly Interspaced Short Palindromic Repeats
MH  - Gene Editing/*methods
MH  - Gene Knockout Techniques/methods
MH  - High-Throughput Screening Assays/methods
MH  - Mutation
MH  - Plants/*genetics
MH  - Plants, Genetically Modified
MH  - Population Density
MH  - RNA, Guide, CRISPR-Cas Systems
PMC - PMC8226290
EDAT- 2021/04/07 06:00
MHDA- 2021/08/21 06:00
PMCR- 2022/04/05
CRDT- 2021/04/06 17:30
PHST- 2021/03/29 00:00 [received]
PHST- 2021/04/02 00:00 [accepted]
PHST- 2021/04/07 06:00 [pubmed]
PHST- 2021/08/21 06:00 [medline]
PHST- 2021/04/06 17:30 [entrez]
PHST- 2022/04/05 00:00 [pmc-release]
AID - 6211199 [pii]
AID - koab099 [pii]
AID - 10.1093/plcell/koab099 [doi]
PST - ppublish
SO  - Plant Cell. 2021 May 31;33(4):794-813. doi: 10.1093/plcell/koab099.