PMID- 33824598
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220421
IS  - 1178-7007 (Print)
IS  - 1178-7007 (Electronic)
IS  - 1178-7007 (Linking)
VI  - 14
DP  - 2021
TI  - Assessing MicroRNA-375 Levels in Type 2 Diabetes Mellitus (T2DM) Patients and 
      Their First-Degree Relatives with T2DM.
PG  - 1445-1451
LID - 10.2147/DMSO.S298735 [doi]
AB  - PURPOSE: The pancreatic islet specific microRNA-375 (miR-375) is overexpressed in 
      type 2 diabetes mellitus (T2DM) patients suppressing the glucose-induced insulin 
      secretion. Thus, miR-375 may serve as a biomarker for the early prediction of 
      T2DM among high-risk individuals. We conducted this clinical study to assess the 
      significance of miR-375 among type 2 diabetes mellitus (T2DM) patients and their 
      first-degree relatives. PATIENTS AND METHODS: We included 56 Han Chinese 
      individuals (N: NGT = 21, T2DM = 10, FD-NGT =13 and FD-T2DM = 12) who received 
      medical health check-ups from January 2018 to September 2018 at The Third 
      Hospital of Yunnan Province, China. They were categorized as normal glucose 
      tolerance (NGT), T2DM, first-degree relatives with normal glucose tolerance 
      (FD-NGT) and first-degree relatives with T2DM (FD-T2DM). OGTT, C-peptide and 
      Insulin tests were performed to confirm the diagnosis. The miR-375 levels were 
      determined by Quantitative real-time RT-PCR (qRT-PCR). RESULTS: The OGTT test 
      showed a significant difference in T2DM and FD-T2DM groups compared with NGT and 
      FD-NGT (p< 0.05). Similar results were observed during C-peptide and insulin 
      tests. Interestingly, the 2-hour insulin test showed FD-NGT group having a 
      significantly higher mean +/- standard error of (64.240 +/- 12.775) compared to NGT 
      (28.836 +/- 10.875). Assessment of miR-375 expression levels in 4 groups showed a 
      significant up-regulation in T2DM and FD-T2DM compared with NGT and FD-NGT 
      groups. A slight increase in miRNA expression was observed in FD-NGT compared 
      with NGT group but was not statistically significant. CONCLUSION: The OGTT, 
      C-peptide and insulin tests revealed a statistically significant difference in 
      T2DM and FD-T2DM compared with NGT and FD-NGT groups. A significantly higher 
      miR-375 expression was also observed in T2DM and FD-T2DM groups compared with NGT 
      and FD-NGT and thus, miR-375 may serve as a stable biomarker for the early 
      prediction of T2DM among high-risk individuals.
CI  - (c) 2021 Wu et al.
FAU - Wu, Xu
AU  - Wu X
AD  - The Third People's Hospital of Yunnan Province, Department of Clinical 
      Laboratory, Kunming, 650200, People's Republic of China.
FAU - Li, Yashan
AU  - Li Y
AD  - The Third People's Hospital of Yunnan Province, Department of Clinical 
      Laboratory, Kunming, 650200, People's Republic of China.
FAU - Man, Baohua
AU  - Man B
AD  - The Third People's Hospital of Yunnan Province, Department of Clinical 
      Laboratory, Kunming, 650200, People's Republic of China.
FAU - Li, Dexuan
AU  - Li D
AD  - The Third People's Hospital of Yunnan Province, Department of Clinical 
      Laboratory, Kunming, 650200, People's Republic of China.
LA  - eng
PT  - Journal Article
DEP - 20210329
PL  - New Zealand
TA  - Diabetes Metab Syndr Obes
JT  - Diabetes, metabolic syndrome and obesity : targets and therapy
JID - 101515585
PMC - PMC8018570
OTO - NOTNLM
OT  - C-peptide
OT  - OGTT
OT  - insulin
OT  - microRNA-375
OT  - type 2 diabetes mellitus
COIS- All authors declare that they have no competing financial interests and report no 
      conflicts of interest in this work.
EDAT- 2021/04/08 06:00
MHDA- 2021/04/08 06:01
PMCR- 2021/03/29
CRDT- 2021/04/07 06:23
PHST- 2020/12/23 00:00 [received]
PHST- 2021/03/04 00:00 [accepted]
PHST- 2021/04/07 06:23 [entrez]
PHST- 2021/04/08 06:00 [pubmed]
PHST- 2021/04/08 06:01 [medline]
PHST- 2021/03/29 00:00 [pmc-release]
AID - 298735 [pii]
AID - 10.2147/DMSO.S298735 [doi]
PST - epublish
SO  - Diabetes Metab Syndr Obes. 2021 Mar 29;14:1445-1451. doi: 10.2147/DMSO.S298735. 
      eCollection 2021.