PMID- 3382643
OWN - NLM
STAT- MEDLINE
DCOM- 19880803
LR  - 20190609
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 941
IP  - 2
DP  - 1988 Jun 22
TI  - Fluidity of intact erythrocyte membranes. Correction for fluorescence energy 
      transfer from diphenylhexatriene to hemoglobin.
PG  - 119-22
AB  - Membranes of intact erythrocytes were labeled by the fluorescent probe 
      1,6-diphenyl-1,3,5-hexatriene (DPH) using an improved labeling procedure 
      described previously (Plasek, J. and Jarolim, P. (1987) Gen. Physiol. Biophys. 6, 
      425-437). The relationship between the steady-state DPH fluorescence anisotropy r 
      and the mean corpuscular hemoglobin concentration (MCHC) was studied. 
      Fluorescence anisotropy increased with increasing MCHC. A linear dependence of r 
      = 0.0026 (MCHC) + 0.113 was obtained which enabled us to measure the fluidity of 
      intact red cell membranes. Without this correction for fluorescence quenching by 
      hemoglobin, incorrect conclusions about membrane fluidity could be made. This 
      fact is demonstrated in a group of pyruvate kinase deficient patients compared 
      with a group of healthy blood donors.
FAU - Plasek, J
AU  - Plasek J
AD  - Institute of Physics of the Charles University, Prague, Czechoslovakia.
FAU - Cermakova, D
AU  - Cermakova D
FAU - Jarolim, P
AU  - Jarolim P
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Hemoglobins)
RN  - 0 (Polyenes)
RN  - 1720-32-7 (Diphenylhexatriene)
RN  - EC 2.7.1.40 (Pyruvate Kinase)
SB  - IM
MH  - Diphenylhexatriene/*blood
MH  - Energy Transfer
MH  - Erythrocyte Indices
MH  - Erythrocyte Membrane/*physiology
MH  - Fluorescence Polarization
MH  - Hemoglobins/*metabolism
MH  - Humans
MH  - *Membrane Fluidity
MH  - Polyenes/*blood
MH  - Pyruvate Kinase/deficiency
OID - NASA: 88252097
EDAT- 1988/06/22 00:00
MHDA- 1988/06/22 00:01
CRDT- 1988/06/22 00:00
PHST- 1988/06/22 00:00 [pubmed]
PHST- 1988/06/22 00:01 [medline]
PHST- 1988/06/22 00:00 [entrez]
AID - 0005-2736(88)90171-X [pii]
AID - 10.1016/0005-2736(88)90171-x [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 1988 Jun 22;941(2):119-22. doi: 
      10.1016/0005-2736(88)90171-x.