PMID- 33827102
OWN - NLM
STAT- MEDLINE
DCOM- 20210624
LR  - 20210624
IS  - 1423-0143 (Electronic)
IS  - 1420-4096 (Linking)
VI  - 46
IP  - 2
DP  - 2021
TI  - Interleukin-6 via Toll-Like Receptor 3 Signaling Attenuates the Expression of 
      Proinflammatory Chemokines in Human Podocytes.
PG  - 207-218
LID - 10.1159/000514589 [doi]
AB  - BACKGROUND: Although toll-like receptor 3 (TLR3) signaling is involved in the 
      development of certain chronic kidney diseases, the specific molecular mechanisms 
      underlying inflammatory reactions via activation of TLR3 signaling in human 
      podocytes remain unclear. Interleukin (IL)-6 is a pleiotropic cytokine associated 
      with innate and adaptive immune responses; however, little is known about the 
      implication of IL-6 via the activation of regional TLR3 signaling in the 
      inflammatory reactions in human podocytes. METHODS: We treated immortalized human 
      podocytes with polyinosinic-polycytidylic acid (poly IC), an authentic viral 
      double-stranded RNA, and assessed the expression of IL-6, monocyte 
      chemoattractant protein-1 (MCP-1), and C-C motif chemokine ligand 5 (CCL5) using 
      quantitative real-time reverse transcription-polymerase chain reaction and 
      enzyme-linked immunosorbent assay. To further elucidate the poly IC-induced 
      signaling pathway, we subjected the cells to RNA interference against IFN-beta and 
      IL-6. RESULTS: We found that the activation of TLR3 induced expression of IL-6, 
      MCP-1, CCL5, and IFN-beta in human podocytes. RNA interference experiments revealed 
      that IFN-beta was involved in the poly IC-induced expression of IL-6, MCP-1, and 
      CCL5. Interestingly, IL-6 knockdown markedly increased the poly IC-induced 
      expression of MCP-1 and CCL5. Further, treatment of cells with IL-6 attenuated 
      the expression of CCL5 and MCP-1 mRNA and proteins. CONCLUSION: IL-6 induced by 
      TLR3 signaling negatively regulates the expression of representative TLR3 
      signaling-dependent proinflammatory chemokines in human podocytes.
CI  - (c) 2021 The Author(s). Published by S. Karger AG, Basel.
FAU - Umetsu, Hidenori
AU  - Umetsu H
AD  - Department of Pediatrics, Hirosaki University Graduate School of Medicine, 
      Hirosaki, Japan.
FAU - Watanabe, Shojiro
AU  - Watanabe S
AD  - Department of Pediatrics, Hirosaki University Graduate School of Medicine, 
      Hirosaki, Japan.
FAU - Imaizumi, Tadaatsu
AU  - Imaizumi T
AD  - Department of Vascular Biology, Hirosaki University Graduate School of Medicine, 
      Hirosaki, Japan.
FAU - Aizawa, Tomomi
AU  - Aizawa T
AD  - Department of Pediatrics, Hirosaki University Graduate School of Medicine, 
      Hirosaki, Japan.
FAU - Tsugawa, Koji
AU  - Tsugawa K
AD  - Department of Pediatrics, Hirosaki University Graduate School of Medicine, 
      Hirosaki, Japan.
FAU - Kawaguchi, Shogo
AU  - Kawaguchi S
AD  - Department of Vascular Biology, Hirosaki University Graduate School of Medicine, 
      Hirosaki, Japan.
FAU - Seya, Kazuhiko
AU  - Seya K
AD  - Department of Vascular Biology, Hirosaki University Graduate School of Medicine, 
      Hirosaki, Japan.
FAU - Matsumiya, Tomoh
AU  - Matsumiya T
AD  - Department of Vascular Biology, Hirosaki University Graduate School of Medicine, 
      Hirosaki, Japan.
FAU - Tanaka, Hiroshi
AU  - Tanaka H
AD  - Department of Pediatrics, Hirosaki University Graduate School of Medicine, 
      Hirosaki, Japan.
AD  - Department of School Health Science, Hirosaki University Faculty of Education, 
      Hirosaki, Japan.
LA  - eng
PT  - Journal Article
DEP - 20210407
PL  - Switzerland
TA  - Kidney Blood Press Res
JT  - Kidney & blood pressure research
JID - 9610505
RN  - 0 (Chemokines)
RN  - 0 (Interleukin-6)
RN  - 0 (Toll-Like Receptor 3)
SB  - IM
MH  - Chemokines/*metabolism
MH  - Humans
MH  - Inflammation/*metabolism
MH  - Interleukin-6/*metabolism
MH  - Podocytes/*metabolism
MH  - Signal Transduction/*immunology
MH  - Toll-Like Receptor 3/*metabolism
OTO - NOTNLM
OT  - C-C motif chemokine ligand 5
OT  - Interleukin-6
OT  - Monocyte chemoattractant protein-1
OT  - Podocytes
OT  - Toll-like receptor 3
EDAT- 2021/04/08 06:00
MHDA- 2021/06/25 06:00
CRDT- 2021/04/07 20:16
PHST- 2020/09/23 00:00 [received]
PHST- 2021/01/20 00:00 [accepted]
PHST- 2021/04/08 06:00 [pubmed]
PHST- 2021/06/25 06:00 [medline]
PHST- 2021/04/07 20:16 [entrez]
AID - 000514589 [pii]
AID - 10.1159/000514589 [doi]
PST - ppublish
SO  - Kidney Blood Press Res. 2021;46(2):207-218. doi: 10.1159/000514589. Epub 2021 Apr 
      7.