PMID- 33833764
OWN - NLM
STAT- MEDLINE
DCOM- 20210924
LR  - 20210924
IS  - 1664-3224 (Electronic)
IS  - 1664-3224 (Linking)
VI  - 12
DP  - 2021
TI  - Allergic Reactions to Serine Protease-Like Proteins of Staphylococcus aureus.
PG  - 651060
LID - 10.3389/fimmu.2021.651060 [doi]
LID - 651060
AB  - In cystic fibrosis (CF) infectious and allergic airway inflammation cause 
      pulmonary exacerbations that destroy the lungs. Staphylococcus aureus is a common 
      long-term colonizer and cause of recurrent airway infections in CF. The pathogen 
      is also associated with respiratory allergy; especially the staphylococcal serine 
      protease-like proteins (Spls) can induce type 2 immune responses in humans and 
      mice. We measured the serum IgE levels specific to 7 proteases of S. aureus by 
      ELISA, targeting 5 Spls (76 CF patients and 46 controls) and the staphopains A 
      and B (16 CF patients and 46 controls). Then we compared cytokine release and 
      phenotype of T cells that had been stimulated with Spls between 5 CF patients and 
      5 controls. CF patients had strongly increased serum IgE binding to all Spls but 
      not to the staphopains. Compared to healthy controls, their Spl-stimulated 
      T cells released more type 2 cytokines (IL-4, IL-5, IL-13) and more IL-6 with no 
      difference in the secretion of type 1- or type 3 cytokines (IFNgamma, IL-17A, 
      IL-17F). IL-10 production was low in CF T cells. The phenotype of the Spl-exposed 
      T cells shifted towards a Th2 or Th17 profile in CF but to a Th1 profile in 
      controls. Sensitization to S. aureus Spls is common in CF. This discovery could 
      explain episodes of allergic inflammation of hitherto unknown causation in CF and 
      extend the diagnostic and therapeutic portfolio.
CI  - Copyright (c) 2021 Nordengrun, Abdurrahman, Treffon, Wachter, Kahl and Broker.
FAU - Nordengrun, Maria
AU  - Nordengrun M
AD  - Department of Immunology, Institute for Immunology and Transfusion Medicine, 
      University Medicine Greifswald, Greifswald, Germany.
FAU - Abdurrahman, Goran
AU  - Abdurrahman G
AD  - Department of Immunology, Institute for Immunology and Transfusion Medicine, 
      University Medicine Greifswald, Greifswald, Germany.
FAU - Treffon, Janina
AU  - Treffon J
AD  - Institute of Medical Microbiology, University Hospital Munster, Munster, Germany.
AD  - Institute of Hygiene, University Hospital Munster, Munster, Germany.
FAU - Wachter, Hannah
AU  - Wachter H
AD  - Institute of Medical Microbiology, University Hospital Munster, Munster, Germany.
FAU - Kahl, Barbara C
AU  - Kahl BC
AD  - Institute of Medical Microbiology, University Hospital Munster, Munster, Germany.
FAU - Broker, Barbara M
AU  - Broker BM
AD  - Department of Immunology, Institute for Immunology and Transfusion Medicine, 
      University Medicine Greifswald, Greifswald, Germany.
LA  - eng
PT  - Journal Article
PT  - Multicenter Study
PT  - Observational Study
PT  - Research Support, Non-U.S. Gov't
DEP - 20210323
PL  - Switzerland
TA  - Front Immunol
JT  - Frontiers in immunology
JID - 101560960
RN  - 0 (Antibodies, Bacterial)
RN  - 0 (Bacterial Proteins)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - EC 3.4.- (Serine Proteases)
SB  - IM
MH  - Adolescent
MH  - Animals
MH  - Antibodies, Bacterial/blood/immunology
MH  - Bacterial Proteins/*immunology/metabolism
MH  - Case-Control Studies
MH  - Cells, Cultured
MH  - Cystic Fibrosis/blood/*immunology/microbiology
MH  - Female
MH  - Healthy Volunteers
MH  - Host-Pathogen Interactions/immunology
MH  - Humans
MH  - Hypersensitivity/blood/immunology/*microbiology
MH  - Immunoglobulin E/blood/immunology
MH  - Male
MH  - Primary Cell Culture
MH  - Serine Proteases/*immunology/metabolism
MH  - Staphylococcal Infections/blood/*immunology/microbiology
MH  - Staphylococcus aureus/enzymology/immunology
MH  - T-Lymphocytes/immunology
MH  - Young Adult
PMC - PMC8021911
OTO - NOTNLM
OT  - IgE
OT  - Staphylococcus aureus
OT  - Th2 cells
OT  - allergy
OT  - cystic fibrosis
OT  - type 2 immune response
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2021/04/10 06:00
MHDA- 2021/09/25 06:00
PMCR- 2021/01/01
CRDT- 2021/04/09 06:26
PHST- 2021/01/08 00:00 [received]
PHST- 2021/03/08 00:00 [accepted]
PHST- 2021/04/09 06:26 [entrez]
PHST- 2021/04/10 06:00 [pubmed]
PHST- 2021/09/25 06:00 [medline]
PHST- 2021/01/01 00:00 [pmc-release]
AID - 10.3389/fimmu.2021.651060 [doi]
PST - epublish
SO  - Front Immunol. 2021 Mar 23;12:651060. doi: 10.3389/fimmu.2021.651060. eCollection 
      2021.