PMID- 33836942
OWN - NLM
STAT- MEDLINE
DCOM- 20220419
LR  - 20220531
IS  - 1995-9133 (Electronic)
IS  - 1684-1182 (Linking)
VI  - 55
IP  - 2
DP  - 2022 Apr
TI  - Accurate detection of oxacillin-resistant Staphylococcus lugdunensis by use of 
      agar dilution.
PG  - 234-240
LID - S1684-1182(21)00053-0 [pii]
LID - 10.1016/j.jmii.2021.02.009 [doi]
AB  - BACKGROUND/PURPOSE: Staphylococcus lugdunensis is a Gram-positive 
      coagulase-negative bacterium and is recognized as a critical pathogenic species 
      recently. Here, we aimed to evaluate the cefoxitin disk diffusion (CDD), 
      oxacillin agar dilution (OAD), and mecA PCR for detecting oxacillin-resistant S. 
      lugdunensis (ORSL) isolates. METHODS: Multilocus sequence typing (MLST) analysis 
      was performed to determine the clonality of 117 S. lugdunensis isolates isolated 
      between May 2009 and Jul 2014. CDD, OAD, and mecA PCR were used to identify 
      oxacillin-resistant S. lugdunensis (ORSL). RESULTS: MLST results showed that the 
      most common sequence type (ST) of our S. lugdunensis isolates was ST6 (35.9%) 
      followed by ST3 (28.2%), ST27 (17.9%), and ST4 (6.8%). CDD and OAD showed that 39 
      and 43 isolates were ORSL, respectively. 4 ST3 CDD-susceptible S. lugdunensis 
      (OSSL) isolates had MIC values >/= 4 for oxacillin. mecA PCR results showed that 43 
      OAD-resistant S. lugdunensis and 3 OAD-susceptible ST27 S. lugdunensis had the 
      mecA gene. Therefore, OAD was used as the gold standard to evaluate the 
      performance of CDD and mecA PCR for identifying ORSL. The overall sensitivity, 
      specificity, and accuracy of CCD for ORSL detection was 90.7%, 100%, and 96.8%, 
      respectively. The sensitivity, specificity, and accuracy of mecA PCR for 
      identifying ORSL was 100%, 95.9%, and 97.44%, respectively. CONCLUSION: Our 
      results indicate that OAD shows higher accuracy for ORSL detection compared with 
      CDD and mecA PCR.
CI  - Copyright (c) 2021. Published by Elsevier B.V.
FAU - Kao, Cheng-Yen
AU  - Kao CY
AD  - Institute of Microbiology and Immunology, School of Life Science, National Yang 
      Ming Chiao Tung University, Taipei, Taiwan.
FAU - Wu, Hsiao-Han
AU  - Wu HH
AD  - Department of Laboratory Medicine, Linkou Chang Gung Memorial Hospital, Taoyuan, 
      Taiwan.
FAU - Chang, Shih-Cheng
AU  - Chang SC
AD  - Department of Laboratory Medicine, Linkou Chang Gung Memorial Hospital, Taoyuan, 
      Taiwan; Department of Medical Biotechnology and Laboratory Science, Chang Gung 
      University, Taoyuan, Taiwan.
FAU - Lin, Lee-Chung
AU  - Lin LC
AD  - Department of Laboratory Medicine, Linkou Chang Gung Memorial Hospital, Taoyuan, 
      Taiwan.
FAU - Liu, Tsui-Ping
AU  - Liu TP
AD  - Department of Laboratory Medicine, Linkou Chang Gung Memorial Hospital, Taoyuan, 
      Taiwan; Department of Medical Biotechnology and Laboratory Science, Chang Gung 
      University, Taoyuan, Taiwan.
FAU - Lu, Jang-Jih
AU  - Lu JJ
AD  - Department of Laboratory Medicine, Linkou Chang Gung Memorial Hospital, Taoyuan, 
      Taiwan; Department of Medical Biotechnology and Laboratory Science, Chang Gung 
      University, Taoyuan, Taiwan; School of Medicine, College of Medicine, Chang Gung 
      University, Taoyuan, Taiwan. Electronic address: janglu45@gmail.com.
LA  - eng
PT  - Journal Article
DEP - 20210323
PL  - England
TA  - J Microbiol Immunol Infect
JT  - Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi
JID - 100956211
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Bacterial Proteins)
RN  - 6OEV9DX57Y (Cefoxitin)
RN  - 9002-18-0 (Agar)
RN  - UH95VD7V76 (Oxacillin)
SB  - IM
MH  - Agar
MH  - Anti-Bacterial Agents/pharmacology
MH  - Bacterial Proteins/analysis/genetics
MH  - Cefoxitin/pharmacology
MH  - *Drug Resistance, Bacterial
MH  - Humans
MH  - Microbial Sensitivity Tests
MH  - Multilocus Sequence Typing
MH  - Oxacillin/*pharmacology
MH  - *Staphylococcal Infections/diagnosis/microbiology
MH  - *Staphylococcus lugdunensis/drug effects/genetics
OTO - NOTNLM
OT  - Agar dilution
OT  - Disk diffusion
OT  - Multilocus sequence typing
OT  - Oxacillin resistance
OT  - SCCmec
OT  - Staphylococcus lugdunensis
COIS- Declaration of competing interest All authors have no conflicts of interest to 
      declare.
EDAT- 2021/04/11 06:00
MHDA- 2022/04/20 06:00
CRDT- 2021/04/10 05:35
PHST- 2020/11/02 00:00 [received]
PHST- 2021/02/21 00:00 [revised]
PHST- 2021/02/28 00:00 [accepted]
PHST- 2021/04/11 06:00 [pubmed]
PHST- 2022/04/20 06:00 [medline]
PHST- 2021/04/10 05:35 [entrez]
AID - S1684-1182(21)00053-0 [pii]
AID - 10.1016/j.jmii.2021.02.009 [doi]
PST - ppublish
SO  - J Microbiol Immunol Infect. 2022 Apr;55(2):234-240. doi: 
      10.1016/j.jmii.2021.02.009. Epub 2021 Mar 23.