PMID- 33838765
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20210419
LR  - 20210419
IS  - 1873-3573 (Electronic)
IS  - 0039-9140 (Linking)
VI  - 229
DP  - 2021 Jul 1
TI  - Simultaneous determination of short-chain fatty alcohols in aged oil and 
      biodiesels by stable isotope labeling assisted liquid chromatography-mass 
      spectrometry.
PG  - 122223
LID - S0039-9140(21)00144-2 [pii]
LID - 10.1016/j.talanta.2021.122223 [doi]
AB  - Short-chain fatty alcohols (SCFAs) are one of the reactants for the production of 
      biodiesels. The SCFA residues at the trace level have a significant impact on 
      biodiesel quality. However, the analysis of SCFAs in aged biodiesels has not been 
      reported so far, which is probably due to the unavailability of an appropriate 
      analytical method for the simultaneous determination of SCFAs. Herein, we 
      developed a novel analytical approach with high sensitivity and selectivity for 
      the simultaneous identification and determination of SCFAs in seed oil and 
      biodiesel samples during the simulated real-time aging by stable isotope labeling 
      assisted liquid chromatography-mass spectrometry (SIL-LC-MS). A pair of isotope 
      labeling reagents, pyridine (Py) and [(2)H(5)]pyridine ([(2)H(5)]Py), were used 
      to label SCFAs in biodiesels and standards, respectively. The [(2)H(5)]Py labeled 
      SCFAs were used as internal standards to compensate for the detection of 
      variances. The simultaneous determination of SCFAs was performed by LC-MS with an 
      improved detection selectivity and sensitivity. The limits of detection (LODs) 
      values were ranged from 0.2 to 0.5 ng mL(-1) for the investigated SCFAs. Good 
      linearity was observed in the studied ranges (R(2) > 0.99) and good precision 
      with relative standard deviations (RSDs) was in the range of 4.9-18.1%. Average 
      recoveries were obtained in the range of 80.3%-115.4%. The matrix effects were in 
      the range of 70.0-104.3%. The validated SIL-LC-MS method was applied to the 
      simultaneous quantitative analysis of SCFAs in seed oil and biodiesel samples and 
      the LC-MS analysis could be done within 3 min. The formation mechanism of SCFAs 
      in aged oil and biodiesel samples was also investigated by this method. The 
      results suggest that SCFAs were formed and their composition changed during the 
      simulated real-time aging of long-chain fatty acid (LCFA), long-chain fatty acid 
      methyl ester (FAME), seed oil, and biodiesels. Moreover, we found that the 
      formation of 1-pentanol and 1-hexanol was associated with the number and position 
      of double bonds in LCFAs and FAMEs.
CI  - Copyright (c) 2021 Elsevier B.V. All rights reserved.
FAU - Xiao, Hua-Ming
AU  - Xiao HM
AD  - Key Laboratory of Analytical Chemistry of the State Ethnic Affairs Commission, 
      College of Chemistry and Materials Science, South-Central University for 
      Nationalities, Wuhan, 430074, People's Republic of China.
FAU - Zhao, Shuai
AU  - Zhao S
AD  - School of Pharmaceutical Engineering ＆; Life Science, Changzhou University, 
      Changzhou, 213164, People's Republic of China.
FAU - Fan, Ru-Ting
AU  - Fan RT
AD  - Key Laboratory of Analytical Chemistry of the State Ethnic Affairs Commission, 
      College of Chemistry and Materials Science, South-Central University for 
      Nationalities, Wuhan, 430074, People's Republic of China.
FAU - Hussain, Dilshad
AU  - Hussain D
AD  - HEJ Research Institute of Chemistry, International Center for Chemical and 
      Biological Sciences, University of Karachi, Karachi, 75270, Pakistan.
FAU - Wang, Xian
AU  - Wang X
AD  - Key Laboratory of Analytical Chemistry of the State Ethnic Affairs Commission, 
      College of Chemistry and Materials Science, South-Central University for 
      Nationalities, Wuhan, 430074, People's Republic of China. Electronic address: 
      xwang27@mail.scuec.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20210309
PL  - Netherlands
TA  - Talanta
JT  - Talanta
JID - 2984816R
SB  - IM
OTO - NOTNLM
OT  - Biodiesel
OT  - Liquid chromatography-tandem mass spectrometry
OT  - Seed oil
OT  - Short-chain fatty alcohols (SCFAs)
OT  - Stable isotope labeling
EDAT- 2021/04/12 06:00
MHDA- 2021/04/12 06:01
CRDT- 2021/04/11 20:19
PHST- 2020/12/16 00:00 [received]
PHST- 2021/02/03 00:00 [revised]
PHST- 2021/02/13 00:00 [accepted]
PHST- 2021/04/11 20:19 [entrez]
PHST- 2021/04/12 06:00 [pubmed]
PHST- 2021/04/12 06:01 [medline]
AID - S0039-9140(21)00144-2 [pii]
AID - 10.1016/j.talanta.2021.122223 [doi]
PST - ppublish
SO  - Talanta. 2021 Jul 1;229:122223. doi: 10.1016/j.talanta.2021.122223. Epub 2021 Mar 
      9.