PMID- 33839399
OWN - NLM
STAT- MEDLINE
DCOM- 20210615
LR  - 20240503
IS  - 1879-1298 (Electronic)
IS  - 0045-6535 (Linking)
VI  - 278
DP  - 2021 Sep
TI  - Biological toxicity assessment of carbamate pesticides using bacterial and plant 
      bioassays: An in-vitro approach.
PG  - 130372
LID - S0045-6535(21)00842-0 [pii]
LID - 10.1016/j.chemosphere.2021.130372 [doi]
AB  - In recent times, agricultural practices mainly rely on agrochemicals and 
      pesticides to safe-guard edible crops against various pests and to ensure high 
      yields. However, their indiscriminate use may cause severe environmental hazards 
      that directly and negatively affect soil microorganisms and crop productivity. 
      Considering these, present study was aimed to assess the toxicity of carbamate 
      pesticides namely carbamoyl (CBL), methomyl (MML) and carbofuran (CBN) using 
      bacterial and plant (Vigna mungo L.) bioassays. All pesticide doses 
      (25-100 mug mL(-1)) showed negative effect on bacteria as well as plant. Growth, 
      morphology, survival, cellular respiration and inner membrane permeability of 
      Sinorhizobiumsaheli was hampered when exposed to pesticides. Pesticide induced 
      morphological changes viz. aberrant margins; cellular cracking and 
      distortion/damage in S. saheli were obvious under scanning electron microscope 
      (SEM). The 100 mugCBNmL(-1) had maximum inhibitory effect and it reduced 
      survivability of S. saheli by 75%. In addition, biofilm formation ability of 
      S. saheli was inhibited in a pesticides-dose dependent manner and it was 
      statistically (p </= 0.05) significant. Pesticides indorsed significant changes in 
      biomarker enzymatic assays and oxidative stress parameters towards S. saheli. 
      Furthermore, at 100 mugCBNmL(-1), germination efficiency, root, shoot length, 
      plant survival and tolerance index of V. mungo were decrease by 50, 75, 65, 70 
      and 66%, respectively over control. Staining of pesticide treated roots with 
      fluorescently labeled dyes propidium iodide (PI) and acridine orange (AO) showed 
      increased oxidative stress, ROS generation and membrane permeability as revealed 
      under confocal laser scanning microscope (CLSM). Furthermore, stressor 
      metabolites and antioxidant enzymes in plant seedlings were progressively 
      enhanced with increasing concentration of pesticides. Conclusively, present 
      finding bestow an insights into a mechanistic approach of carbamate pesticide 
      induced phyto, morpho and cellular toxic effects towards soil bacterium as well 
      as plant with forthcoming implications for designing the pesticides to reduce 
      their toxic/harmful effects.
CI  - Copyright (c) 2021 Elsevier Ltd. All rights reserved.
FAU - Shahid, Mohammad
AU  - Shahid M
AD  - Department of Agricultural Microbiology, Faculty of Agricultural Sciences, 
      Aligarh Muslim University, Aligarh, 202002, Uttar Pradesh, India. Electronic 
      address: shahidfaiz5@gmail.com.
FAU - Manoharadas, Salim
AU  - Manoharadas S
AD  - Department of Botany and Microbiology, Central Laboratory, College of Science, 
      King Saud University, PO Box 2455, Riyadh 11451, Saudi Arabia. Electronic 
      address: smanoharadas@ksu.edu.sa.
FAU - Chakdar, Hillol
AU  - Chakdar H
AD  - Microbial Technology Unit II, ICAR-National Bureau of Agriculturally Important 
      Microorganisms (NBAIM), Mau, Uttar Pradesh, 275103, India.
FAU - Alrefaei, Abdulwahed F
AU  - Alrefaei AF
AD  - Department of Zoology, College of Science, King Saud University, P.O. Box. 2454, 
      Riyadh, 11451, Saudi Arabia.
FAU - Albeshr, Mohammed F
AU  - Albeshr MF
AD  - Department of Zoology, College of Science, King Saud University, P.O. Box. 2454, 
      Riyadh, 11451, Saudi Arabia.
FAU - Almutairi, Mikhlid H
AU  - Almutairi MH
AD  - Department of Zoology, College of Science, King Saud University, P.O. Box. 2454, 
      Riyadh, 11451, Saudi Arabia.
LA  - eng
PT  - Journal Article
DEP - 20210402
PL  - England
TA  - Chemosphere
JT  - Chemosphere
JID - 0320657
RN  - 0 (Carbamates)
RN  - 0 (Pesticides)
SB  - IM
ECI - Chemosphere. 2024 May;356:142020. PMID: 38685660
MH  - Bacteria
MH  - Biological Assay
MH  - Carbamates/toxicity
MH  - *Pesticides/toxicity
MH  - Plants
OTO - NOTNLM
OT  - Carbamate pesticides
OT  - Cellular respiration
OT  - Growth inhibition
OT  - Inner membrane permeability
OT  - Oxidative stress
OT  - Toxicity assessment
EDAT- 2021/04/12 06:00
MHDA- 2021/06/16 06:00
CRDT- 2021/04/11 20:35
PHST- 2020/12/18 00:00 [received]
PHST- 2021/03/16 00:00 [revised]
PHST- 2021/03/19 00:00 [accepted]
PHST- 2021/04/12 06:00 [pubmed]
PHST- 2021/06/16 06:00 [medline]
PHST- 2021/04/11 20:35 [entrez]
AID - S0045-6535(21)00842-0 [pii]
AID - 10.1016/j.chemosphere.2021.130372 [doi]
PST - ppublish
SO  - Chemosphere. 2021 Sep;278:130372. doi: 10.1016/j.chemosphere.2021.130372. Epub 
      2021 Apr 2.