PMID- 33839419
OWN - NLM
STAT- MEDLINE
DCOM- 20210720
LR  - 20210720
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 556
DP  - 2021 Jun 4
TI  - TRIM37 negatively regulates inflammatory responses induced by virus infection via 
      controlling TRAF6 ubiquitination.
PG  - 87-92
LID - S0006-291X(21)00558-1 [pii]
LID - 10.1016/j.bbrc.2021.03.147 [doi]
AB  - Virus-induced cytokine storm has been a devastating actuality in clinic. The 
      abnormal production of type I interferon (IFN-1) and upregulation of multiple 
      cytokines induced strong inflammation and thus lead to shock and organ failure. 
      As an E3 ubiquitin ligase, tripartite motif-containing 37 (TRIM37) regulates the 
      ubiquitination of multiple proteins including TRAFs. RNA sequencing was performed 
      to investigated the alteration of transcriptional profile of H1N1-infected 
      patients. qRT-PCR assay was performed to investigate the RNA levels of certain 
      genes. The group of immune cells was examined by the Flow cytometry analysis. H&E 
      staining was applied to evaluate lung inflammation of WT and TRIM37-KO mice. 
      ELISA assay was performed to demonstrate the alteration of multiple cytokines. 
      The protein levels in NF-kB signaling was estimated by western blotting and 
      immunoprecipitation assays were applied to demonstrate the direct interaction 
      between TRIM37 and TRAF-6. The RNA level of TRIM37 decreased in CD11b(+) cells of 
      Flu-infected patients. Knockout of TRIM37 inhibited the immune responses of 
      H1N1-infected mice. TRIM37 deficiency reduced the levels of virous 
      proinflammatory cytokines in bone marrow derived macrophages (BMDMs). 
      Mechanically, TRIM37 promoted the K63-linked ubiquitination of TRAF6. TRIM37 
      negatively regulated inflammatory responses induced by virus infection via 
      promoting TRAF6 ubiquitination at K63.
CI  - Copyright (c) 2021 Elsevier Inc. All rights reserved.
FAU - Zhao, Lifen
AU  - Zhao L
AD  - Department of Respiratory and Critical Care Medicine, Shanxi Bethune Hospital, 
      Taiyuan, 030032, Shanxi, China.
FAU - Hao, Yanyan
AU  - Hao Y
AD  - Department of Respiratory and Critical Care Medicine, Shanxi Bethune Hospital, 
      Taiyuan, 030032, Shanxi, China.
FAU - Song, Zhuohui
AU  - Song Z
AD  - Department of Physiology, Changzhi Medical College, No.161 Jiefang East Street, 
      Changzhi, 046000, Shanxi, China.
FAU - Fan, Yimin
AU  - Fan Y
AD  - Functional Comprehensive Laboratory, Changzhi Medical College, No.161 Jiefang 
      East Street, Changzhi, 046000, Shanxi, China.
FAU - Li, Shufen
AU  - Li S
AD  - Department of Physiology, Changzhi Medical College, No.161 Jiefang East Street, 
      Changzhi, 046000, Shanxi, China. Electronic address: lsfddcy@163.com.
LA  - eng
PT  - Journal Article
DEP - 20210408
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Inflammation Mediators)
RN  - 0 (TNF Receptor-Associated Factor 6)
RN  - 0 (Tripartite Motif Proteins)
RN  - EC 2.3.2.27 (TRIM37 protein, human)
RN  - EC 2.3.2.27 (TRIM37 protein, mouse)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
SB  - IM
MH  - Animals
MH  - Female
MH  - Humans
MH  - Inflammation/*metabolism
MH  - Inflammation Mediators/antagonists & inhibitors/immunology
MH  - Influenza A Virus, H1N1 Subtype/immunology
MH  - Influenza, Human/genetics/immunology/metabolism/virology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Orthomyxoviridae Infections/genetics/immunology/*metabolism/*virology
MH  - TNF Receptor-Associated Factor 6/chemistry/*metabolism
MH  - Tripartite Motif Proteins/deficiency/genetics/*metabolism
MH  - Ubiquitin-Protein Ligases/deficiency/genetics/*metabolism
MH  - *Ubiquitination
OTO - NOTNLM
OT  - Interferon
OT  - Tripartite motif-containing 37 (TRIM37)
OT  - Ubiquitination
OT  - Viral infection
COIS- Declaration of competing interest The authors declare that they have no conflict 
      of interest.
EDAT- 2021/04/12 06:00
MHDA- 2021/07/21 06:00
CRDT- 2021/04/11 20:35
PHST- 2021/03/24 00:00 [received]
PHST- 2021/03/26 00:00 [accepted]
PHST- 2021/04/12 06:00 [pubmed]
PHST- 2021/07/21 06:00 [medline]
PHST- 2021/04/11 20:35 [entrez]
AID - S0006-291X(21)00558-1 [pii]
AID - 10.1016/j.bbrc.2021.03.147 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2021 Jun 4;556:87-92. doi: 
      10.1016/j.bbrc.2021.03.147. Epub 2021 Apr 8.