PMID- 33841160
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210413
IS  - 1663-9812 (Print)
IS  - 1663-9812 (Electronic)
IS  - 1663-9812 (Linking)
VI  - 12
DP  - 2021
TI  - Nicotinamide Mononucleotide Combined With Lactobacillus fermentum TKSN041 Reduces 
      the Photoaging Damage in Murine Skin by Activating AMPK Signaling Pathway.
PG  - 643089
LID - 10.3389/fphar.2021.643089 [doi]
LID - 643089
AB  - Long-term exposure to UVB (280-320 nm) can cause oxidative skin damage, 
      inflammatory injury, and skin cancer. Research on nicotinamide mononucleotide 
      (NMN) and lactic acid bacteria (LAB) with regard to antioxidation, 
      anti-inflammation, and prevention of other age-related diseases has received 
      increasing attention. In the present study, the in vitro antioxidant analysis 
      showed that NMN combined with Lactobacillus fermentum TKSN041 (L. fermentum 
      TKSN041) has a high scavenging ability on hydroxyl (OH), 2, 2'-azino-bis 
      (3-ethylbenzthiazoline-6-sulphonic acid) diammonium salt (ABTS) and 1, 
      1-diphenyl-2-picrylhydrazyl (DPPH), and it also possess a good total antioxidant 
      capacity. The animal experimental results show that NMN combined with LAB 
      maintained normal liver morphology of mice and reduced pathological damage to 
      murine skin. NMN combined with LAB significantly increased the serum levels of 
      total superoxide dismutase (T-SOD), catalase (CAT), and interleukin (IL)-10, but 
      reduced the levels of malondialdehyde, advanced glycation end products, tumor 
      necrosis factor (TNF)-alpha, and IL-6. NMN combined with LAB increased T-SOD, CAT, 
      IL-10, Na(+)-K(+)-ATPase, and NAD(+) levels in the skin, but reduced TNF-alpha level 
      in the skin. NMN combined with LAB increased the mRNA expression levels of SOD1, 
      CAT, glutathione (GSH), inhibitor of NF-kappaB (IkappaB-alpha), IL-10, AMP-activated protein 
      kinase (AMPK), adaptor protein, phosphotyros ineinteraction, PH domain and 
      leucine zipper containing 1 (APPL1), peroxisome proliferator-activated receptor gamma 
      co-activator-1alpha (PGC-1alpha), and forkhead transcription factor O (FOXO) in the skin 
      and liver, but decreased the mRNA expression levels of nuclear factor (NF)-kappaBp65, 
      TNF-alpha, IL-6, and rapamycin target protein (mTOR). NMN combined with LAB increased 
      the protein expression levels of AMPK, IkappaB-alpha, SOD1, and CAT in the skin tissues 
      and reduced protein expression of NF-kappaBp65. NMN combined with L. fermentum 
      TKSN041 improved murine skin damage caused by UVB irradiation, and the protective 
      mechanism may be related to activation of the AMPK signaling pathway. The results 
      of this study are expected to provide a reference for preventing and the treating 
      skin photoaging.
CI  - Copyright (c) 2021 Zhou, Du, Ni, Ran, Hu, Yu and Zhao.
FAU - Zhou, Xianrong
AU  - Zhou X
AD  - Chongqing Collaborative Innovation Center for Functional Food, Chongqing 
      University of Education, Chongqing, China.
AD  - Chongqing Engineering Research Center of Functional Food, Chongqing University of 
      Education, Chongqing, China.
AD  - Chongqing Engineering Laboratory for Research and Development of Functional Food, 
      Chongqing University of Education, Chongqing, China.
FAU - Du, Hang-Hang
AU  - Du HH
AD  - Department of Plastic Surgery, Chongqing Huamei Plastic Surgery Hospital, 
      Chongqing, China.
FAU - Ni, Luyao
AU  - Ni L
AD  - Chongqing Collaborative Innovation Center for Functional Food, Chongqing 
      University of Education, Chongqing, China.
FAU - Ran, Jie
AU  - Ran J
AD  - Chongqing Collaborative Innovation Center for Functional Food, Chongqing 
      University of Education, Chongqing, China.
FAU - Hu, Jian
AU  - Hu J
AD  - Effepharm (Shanghai) Co., Ltd., Shanghai, China.
FAU - Yu, Jianjun
AU  - Yu J
AD  - Effepharm (Shanghai) Co., Ltd., Shanghai, China.
FAU - Zhao, Xin
AU  - Zhao X
AD  - Chongqing Collaborative Innovation Center for Functional Food, Chongqing 
      University of Education, Chongqing, China.
AD  - Chongqing Engineering Research Center of Functional Food, Chongqing University of 
      Education, Chongqing, China.
AD  - Chongqing Engineering Laboratory for Research and Development of Functional Food, 
      Chongqing University of Education, Chongqing, China.
LA  - eng
PT  - Journal Article
DEP - 20210325
PL  - Switzerland
TA  - Front Pharmacol
JT  - Frontiers in pharmacology
JID - 101548923
PMC - PMC8027253
OTO - NOTNLM
OT  - AMP
OT  - UVB
OT  - in vitro antioxidant
OT  - lactobacillus fermentum
OT  - nicotinamide mononucleotide
OT  - skin
COIS- Jian Hu and Jianjun Yu were employed by Effepharm (Shanghai) Co., Ltd. The 
      remaining authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2021/04/13 06:00
MHDA- 2021/04/13 06:01
PMCR- 2021/03/25
CRDT- 2021/04/12 06:16
PHST- 2020/12/17 00:00 [received]
PHST- 2021/02/15 00:00 [accepted]
PHST- 2021/04/12 06:16 [entrez]
PHST- 2021/04/13 06:00 [pubmed]
PHST- 2021/04/13 06:01 [medline]
PHST- 2021/03/25 00:00 [pmc-release]
AID - 643089 [pii]
AID - 10.3389/fphar.2021.643089 [doi]
PST - epublish
SO  - Front Pharmacol. 2021 Mar 25;12:643089. doi: 10.3389/fphar.2021.643089. 
      eCollection 2021.