PMID- 33841686
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210413
IS  - 1943-8141 (Print)
IS  - 1943-8141 (Electronic)
IS  - 1943-8141 (Linking)
VI  - 13
IP  - 3
DP  - 2021
TI  - LINC01303 promotes the proliferation and migration of laryngeal carcinoma by 
      regulating miR-200c/TIMP2 axis.
PG  - 1643-1656
AB  - BACKGROUND: It is reported that long non-coding RNA is crucial in many cancer 
      progressions. But the function and regulatory mechanism of LINC01303 in human 
      laryngeal squamous cell carcinoma (LSCC) remains unclear. Hence, this research 
      aims at investigating the biological function and potential mechanism of 
      LINC01303 in LSCC. METHODS: Real-time quantitative PCR (qRT-PCR) was applied for 
      the determination of LINC01303, miR-200c and TIMP metallopeptidase inhibitor 2 
      (TIMP2) expression in LSCC tissues and cell lines. Corresponding experiments were 
      carried out to determine the impacts of LINC01303 on LSCC cell proliferation, 
      apoptosis, migration and invasion. The interaction between LINC01303 and miR-200c 
      was analyzed with bioinformatics analysis and luciferase activity analysis. 
      RESULTS: LINC01303 expression in LSCC tissues was notably higher than that in 
      adjacent normal tissues. High LINC01303 expression was bound up with lymphatic 
      metastasis and advanced clinical stage. In addition, inhibition of LINC01303 by 
      siRNA could evidently block LSCC cell proliferation, induce apoptosis, and 
      inhibit invasion and migration. Mechanically, LINC01303 acted as carcinogenic 
      lncRNA in LSCC by regulating miR-200c/TIMP2 axis. CONCLUSION: LINC01303 plays a 
      carcinogenic part in LSCC carcinogenesis through regulating miR-200c/TIMP2 axis, 
      which may become a promising target of LSCC therapy.
CI  - AJTR Copyright (c) 2021.
FAU - Xiao, Dong
AU  - Xiao D
AD  - Department of Otolaryngology-Head and Neck Surgery, The First Hospital of Jilin 
      University Changchun 130021, People's Republic of China.
FAU - Cui, Xiangyan
AU  - Cui X
AD  - Department of Otolaryngology-Head and Neck Surgery, The First Hospital of Jilin 
      University Changchun 130021, People's Republic of China.
FAU - Fang, Ning
AU  - Fang N
AD  - Department of Otolaryngology-Head and Neck Surgery, The First Hospital of Jilin 
      University Changchun 130021, People's Republic of China.
FAU - Yu, Shujian
AU  - Yu S
AD  - Department of Otolaryngology-Head and Neck Surgery, The First Hospital of Jilin 
      University Changchun 130021, People's Republic of China.
FAU - Wang, Xin
AU  - Wang X
AD  - Department of Otolaryngology-Head and Neck Surgery, The First Hospital of Jilin 
      University Changchun 130021, People's Republic of China.
LA  - eng
PT  - Journal Article
DEP - 20210315
PL  - United States
TA  - Am J Transl Res
JT  - American journal of translational research
JID - 101493030
PMC - PMC8014399
OTO - NOTNLM
OT  - LINC01303
OT  - Laryngeal squamous cell carcinoma
OT  - TIMP2
OT  - long non-coding RNA
OT  - miR-200c
COIS- None.
EDAT- 2021/04/13 06:00
MHDA- 2021/04/13 06:01
PMCR- 2021/03/15
CRDT- 2021/04/12 06:19
PHST- 2020/10/27 00:00 [received]
PHST- 2020/12/08 00:00 [accepted]
PHST- 2021/04/12 06:19 [entrez]
PHST- 2021/04/13 06:00 [pubmed]
PHST- 2021/04/13 06:01 [medline]
PHST- 2021/03/15 00:00 [pmc-release]
PST - epublish
SO  - Am J Transl Res. 2021 Mar 15;13(3):1643-1656. eCollection 2021.