PMID- 33847822
OWN - NLM
STAT- MEDLINE
DCOM- 20211116
LR  - 20211116
IS  - 1559-131X (Electronic)
IS  - 1357-0560 (Linking)
VI  - 38
IP  - 5
DP  - 2021 Apr 13
TI  - The CXCL12/CXCR7 signalling axis promotes proliferation and metastasis in 
      cervical cancer.
PG  - 58
LID - 10.1007/s12032-021-01481-2 [doi]
AB  - C-X-C chemokine receptor 7 (CXCR7), a novel receptor of C-X-C motif chemokine 
      ligand 12 (CXCL12), is associated with the occurrence and metastasis of various 
      malignant tumours. However, the role, function and underlying mechanisms of CXCR7 
      expression in cervical cancer remain undefined. The expression level of CXCR7 was 
      evaluated in cervical cancer samples by immunohistochemistry and real-time PCR 
      analyses. Western blot analysis was used to examine the expression level of CXCR7 
      in cervical cancer cell lines. HeLa cells were genetically silenced or 
      pharmacologically inhibited for CXCR7 or CXCR4. Transwell and CCK-8 assays were 
      used to examine cell migration and proliferation. The expression levels of MMP2, 
      MMP9, TIMP-1 and TIMP-2 in HeLa cells were assessed by western blot or real-time 
      PCR. HeLa cells silenced for CXCR7 were subcutaneously injected into nude mice to 
      form tumours. The expression of CXCR7 in nude mice was investigated by 
      immunohistochemical staining. Tumour volumes and weights were measured. The in 
      vivo expression levels of MMP2, MMP9, TIMP-1 and TIMP-2 were determined by 
      western blot analysis and real-time PCR. CXCR7 was overexpressed in cervical 
      cancer tissues and cell lines. CXCL12 was highly expressed in cervical cancer 
      lines. CXCR7 silencing or CCX733 treatment rather than CXCR4 silencing or AMD3100 
      treatment suppressed the proliferation, migration and invasion of cervical cancer 
      cells stimulated by CXCL12. In a xenograft tumour model, CXCR7 silencing or 
      CCX733 treatment inhibited the volumes and weights of xenograft tumours. In 
      addition, downregulation of CXCR7 decreased the expression levels of MMP2 and 
      MMP9 but increased the expression levels of TIMP-1 and TIMP-2 in vivo. These data 
      support the finding that the downregulation of CXCR7 suppresses the proliferation 
      and metastasis of cervical cancer cells. Inhibition of CXCR7 may be a potential 
      targeted therapy for cervical cancer.
FAU - Xu, Leilei
AU  - Xu L
AUID- ORCID: 0000-0001-7045-4567
AD  - Department of Gynecology, The First Hospital of Huai'an Affiliated to Nanjing 
      Medical University, West Beijing Road, Huai'an, 223300, Jiangsu, China. 
      leileixu@tom.com.
FAU - Li, Changhua
AU  - Li C
AD  - Department of Gynecology, The First Hospital of Huai'an Affiliated to Nanjing 
      Medical University, West Beijing Road, Huai'an, 223300, Jiangsu, China.
FAU - Hua, Fu
AU  - Hua F
AD  - Department of Gynecology, The First Hospital of Huai'an Affiliated to Nanjing 
      Medical University, West Beijing Road, Huai'an, 223300, Jiangsu, China.
FAU - Liu, Xiaoping
AU  - Liu X
AD  - Department of Gynecology, The First Hospital of Huai'an Affiliated to Nanjing 
      Medical University, West Beijing Road, Huai'an, 223300, Jiangsu, China.
LA  - eng
PT  - Journal Article
DEP - 20210413
PL  - United States
TA  - Med Oncol
JT  - Medical oncology (Northwood, London, England)
JID - 9435512
RN  - 0 (ACKR3 protein, human)
RN  - 0 (CXCL12 protein, human)
RN  - 0 (CXCR4 protein, human)
RN  - 0 (Chemokine CXCL12)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Receptors, CXCR)
RN  - 0 (Receptors, CXCR4)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
SB  - IM
MH  - Animals
MH  - Carcinoma, Squamous Cell/drug therapy/*pathology
MH  - Cell Division
MH  - Cell Line
MH  - Cell Line, Tumor
MH  - Cervix Uteri/cytology
MH  - Chemokine CXCL12/*physiology
MH  - Epithelial Cells/metabolism
MH  - Female
MH  - Humans
MH  - Matrix Metalloproteinases/biosynthesis
MH  - Mice
MH  - Mice, Nude
MH  - Molecular Targeted Therapy
MH  - Neoplasm Invasiveness
MH  - Neoplasm Metastasis
MH  - Neoplasm Proteins/antagonists & inhibitors/*physiology
MH  - RNA Interference
MH  - RNA, Small Interfering/genetics
MH  - Receptors, CXCR/antagonists & inhibitors/*physiology
MH  - Receptors, CXCR4/antagonists & inhibitors/genetics/physiology
MH  - Signal Transduction/*physiology
MH  - Uterine Cervical Neoplasms/drug therapy/*pathology
MH  - Xenograft Model Antitumor Assays
OTO - NOTNLM
OT  - CXCL12
OT  - CXCR7
OT  - Cervical cancer
OT  - Metastasis
EDAT- 2021/04/14 06:00
MHDA- 2021/11/17 06:00
CRDT- 2021/04/13 12:17
PHST- 2020/12/08 00:00 [received]
PHST- 2021/02/13 00:00 [accepted]
PHST- 2021/04/13 12:17 [entrez]
PHST- 2021/04/14 06:00 [pubmed]
PHST- 2021/11/17 06:00 [medline]
AID - 10.1007/s12032-021-01481-2 [pii]
AID - 10.1007/s12032-021-01481-2 [doi]
PST - epublish
SO  - Med Oncol. 2021 Apr 13;38(5):58. doi: 10.1007/s12032-021-01481-2.