PMID- 33847993
OWN - NLM
STAT- MEDLINE
DCOM- 20210810
LR  - 20210810
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 2307
DP  - 2021
TI  - Yarrowia lipolytica Bioprocess Development: From Flask to Bioreactor.
PG  - 221-232
LID - 10.1007/978-1-0716-1414-3_15 [doi]
AB  - Yarrowia lipolytica produces a range of valuable biotechnological products from 
      natural metabolites and enzymes to heterologous proteins. The production of these 
      products is affected by medium composition and various environmental factors. 
      Here we describe bioprocess development for a recombinant laccase production by 
      Y. lipolytica. At first, response surface methodology (RSM), as a statistical 
      technique for design of experiment (DOE), is used for the optimization of medium 
      composition in flask level. Then, results of RSM are applied to increase laccase 
      production in controlled conditions of the bioreactor.
FAU - Darvishi, Farshad
AU  - Darvishi F
AD  - Department of Microbiology, Faculty of Biological Sciences, Alzahra University, 
      Tehran, Iran. f.darvishi@ymail.com.
AD  - Microbial Biotechnology and Bioprocess Engineering (MBBE) Group, Department of 
      Microbiology, Faculty of Science, University of Maragheh, Maragheh, Iran. 
      f.darvishi@ymail.com.
FAU - Moradi, Marzieh
AU  - Moradi M
AD  - Microbial Biotechnology and Bioprocess Engineering (MBBE) Group, Department of 
      Microbiology, Faculty of Science, University of Maragheh, Maragheh, Iran.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Fungal Proteins)
RN  - 0 (Recombinant Proteins)
RN  - EC 1.10.3.2 (Laccase)
SB  - IM
MH  - Batch Cell Culture Techniques/*methods
MH  - Bioreactors/*microbiology
MH  - Fungal Proteins/genetics
MH  - Laccase/*genetics/metabolism
MH  - Metabolic Engineering
MH  - Recombinant Proteins/metabolism
MH  - Yarrowia/genetics/*growth & development/metabolism
OTO - NOTNLM
OT  - Bioprocess
OT  - Bioreactor
OT  - Design of experiments
OT  - Optimization
OT  - Yarrowia lipolytica
EDAT- 2021/04/14 06:00
MHDA- 2021/08/11 06:00
CRDT- 2021/04/13 12:21
PHST- 2021/04/13 12:21 [entrez]
PHST- 2021/04/14 06:00 [pubmed]
PHST- 2021/08/11 06:00 [medline]
AID - 10.1007/978-1-0716-1414-3_15 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2021;2307:221-232. doi: 10.1007/978-1-0716-1414-3_15.