PMID- 33848916
OWN - NLM
STAT- MEDLINE
DCOM- 20210617
LR  - 20210617
IS  - 1873-264X (Electronic)
IS  - 0731-7085 (Linking)
VI  - 199
DP  - 2021 May 30
TI  - Development and validation of a sensitive LC-MS/MS method for simultaneous 
      quantification of thirteen steroid hormones in human serum and its application to 
      the study of type 2 diabetes mellitus.
PG  - 114059
LID - S0731-7085(21)00170-9 [pii]
LID - 10.1016/j.jpba.2021.114059 [doi]
AB  - Endogenous steroid hormones with similar structure, poor content and high 
      efficacy are difficult and vital to be quantitatively detected. In this study, a 
      validated method was established for the simultaneous quantification of thirteen 
      steroids in human serum, and applied to the study of type 2 diabetes mellitus 
      (T2DM). An ultra-high performance liquid chromatography coupled with triple 
      quadrupole mass spectrometry (UPLC-MS/MS) was developed for the simultaneous 
      determination of thirteen steroid hormones in human serum, including 
      androstenedione, corticosterone (B), cortisol (F), cortisone, 18-hydroxycortisol 
      (18OHF), 11-deoxycorticosterone, 11-deoxycortisol, pregnenolone, progesterone, 
      17-hydroxyprogesterone, testosterone, androstanolone and estradiol. Under the 
      optimum conditions, method was achieved with a BEH Shield RP18 column within 
      18 min. The lower limits of quantitation for steroids were 0.08-7.81 ng/mL. The 
      intra- and inter-day precision for all the analytes were less than 15 %, and the 
      accuracy ranged from -14.19 % to 12.89 % at three quality control levels. The 
      proposed method, indicating high steady and sensitivity, was successfully applied 
      to the quantification of thirteen steroids levels in serum from patients with 
      T2DM and healthy individuals. The serum concentrations of 18OHF and F were 
      significantly increased in the patients compared with the healthy individuals, 
      while B was significantly decreased. The fold change was 1.98, 1.25 and 0.79 
      respectively. The ratio of 18OHF to B (18OHF/B) exhibited a 2.51-fold increase in 
      T2DM patients and presented a more significant change. 18OHF/B was identified as 
      a prospective serum marker, which deserves further attention.
CI  - Copyright (c) 2021 Elsevier B.V. All rights reserved.
FAU - Liu, Wuwei
AU  - Liu W
AD  - Department of Pharmacology and Chemical Biology, Shanghai Jiao Tong University 
      School of Medicine, Shanghai, 200025, PR China.
FAU - Yuan, Daoyi
AU  - Yuan D
AD  - Department of Pharmacology and Chemical Biology, Shanghai Jiao Tong University 
      School of Medicine, Shanghai, 200025, PR China.
FAU - Han, Minlu
AU  - Han M
AD  - Department of Pharmacology and Chemical Biology, Shanghai Jiao Tong University 
      School of Medicine, Shanghai, 200025, PR China.
FAU - Huang, Jingwen
AU  - Huang J
AD  - Department of Respiratory and Critical Care Medicine, Ruijin Hospital, Shanghai 
      Jiao Tong University School of Medicine, Shanghai, 200025, PR China.
FAU - Xie, Ying
AU  - Xie Y
AD  - Department of Pharmacology and Chemical Biology, Shanghai Jiao Tong University 
      School of Medicine, Shanghai, 200025, PR China. Electronic address: 
      xyshirlley@msn.com.
LA  - eng
PT  - Journal Article
DEP - 20210405
PL  - England
TA  - J Pharm Biomed Anal
JT  - Journal of pharmaceutical and biomedical analysis
JID - 8309336
RN  - 0 (Steroids)
SB  - IM
MH  - Chromatography, Liquid
MH  - *Diabetes Mellitus, Type 2/drug therapy
MH  - Humans
MH  - Limit of Detection
MH  - Prospective Studies
MH  - Steroids
MH  - Tandem Mass Spectrometry
OTO - NOTNLM
OT  - Human serum
OT  - Liquid chromatography-tandem mass spectrometry
OT  - Steroids
OT  - Type 2 diabetes mellitus
COIS- Declaration of Competing Interest The authors declare that they have no competing 
      interests.
EDAT- 2021/04/14 06:00
MHDA- 2021/06/22 06:00
CRDT- 2021/04/13 20:25
PHST- 2021/02/07 00:00 [received]
PHST- 2021/03/30 00:00 [revised]
PHST- 2021/03/31 00:00 [accepted]
PHST- 2021/04/14 06:00 [pubmed]
PHST- 2021/06/22 06:00 [medline]
PHST- 2021/04/13 20:25 [entrez]
AID - S0731-7085(21)00170-9 [pii]
AID - 10.1016/j.jpba.2021.114059 [doi]
PST - ppublish
SO  - J Pharm Biomed Anal. 2021 May 30;199:114059. doi: 10.1016/j.jpba.2021.114059. 
      Epub 2021 Apr 5.