PMID- 33849004
OWN - NLM
STAT- MEDLINE
DCOM- 20211025
LR  - 20211025
IS  - 1758-5090 (Electronic)
IS  - 1758-5082 (Linking)
VI  - 13
IP  - 3
DP  - 2021 May 5
TI  - An engineered neurovascular unit for modeling neuroinflammation.
LID - 10.1088/1758-5090/abf741 [doi]
AB  - The neurovascular unit (NVU) comprises multiple types of brain cells, including 
      brain endothelial cells, astrocytes, pericytes, neurons, microglia, and 
      oligodendrocytes. Each cell type contributes to the maintenance of the molecular 
      transport barrier and brain tissue homeostasis. Several disorders and diseases of 
      the central nervous system, including neuroinflammation, Alzheimer's disease, 
      stroke, and multiple sclerosis, have been associated with dysfunction of the NVU. 
      As a result, there has been increased demand for the development of NVUin 
      vitromodels. Here, we present a three-dimensional (3D) immortalized human 
      cell-based NVU model generated by organizing the brain microvasculature in a 
      collagen matrix embedded with six different types of cells that comprise the NVU. 
      By surrounding a perfusable brain endothelium with six types of NVU-composing 
      cells, we demonstrated a significant impact of the 3D co-culture on the 
      maturation of barrier function, which is supported by cytokines secreted from 
      NVU-composing cells. Furthermore, NVU-composing cells alleviated the inflammatory 
      responses induced by lipopolysaccharides. Our human cell-based NVUin vitromodel 
      could enable elucidation of both physiological and pathological mechanisms in the 
      human brain and evaluation of safety and efficacy in the context of high-content 
      analysis during the process of drug development.
CI  - (c) 2021 IOP Publishing Ltd.
FAU - Seo, Suyeong
AU  - Seo S
AD  - Brain Science Institute, Korea Institute of Science and Technology (KIST), Seoul 
      02792, Republic of Korea.
AD  - Program in Nano Science and Technology, Graduate School of Convergence Science 
      and Technology, Seoul National University, Seoul 08826, Republic of Korea.
AD  - These authors contributed equally to this work.
FAU - Choi, Chi-Hoon
AU  - Choi CH
AD  - Department of Radiology, Chung Buk National University Hospital, Cheongju, Chung 
      Buk, Republic of Korea.
AD  - College of Medicine, Chung Buk National University, Cheongju, Chung Buk 28644, 
      Republic of Korea.
AD  - These authors contributed equally to this work.
FAU - Yi, Kyung Sik
AU  - Yi KS
AD  - Department of Radiology, Chung Buk National University Hospital, Cheongju, Chung 
      Buk, Republic of Korea.
FAU - Kim, Seung U
AU  - Kim SU
AD  - Division of Neurology, Department of Medicine, UBC Hospital, University of 
      British Columbia, Vancouver, BC, Canada.
FAU - Lee, Kangwon
AU  - Lee K
AD  - Department of Applied Bioengineering, Graduate School of Convergence Science and 
      Technology, Seoul National University, Seoul 08826, Republic of Korea.
FAU - Choi, Nakwon
AU  - Choi N
AUID- ORCID: 0000-0003-2993-9233
AD  - Brain Science Institute, Korea Institute of Science and Technology (KIST), Seoul 
      02792, Republic of Korea.
AD  - Division of Bio-Medical Science and Technology, KIST School, Korea University of 
      Science and Technology (UST), Seoul 02792, Republic of Korea.
AD  - KU-KIST Graduate School of Converging Science and Technology, Korea University, 
      Seoul 02841, Republic of Korea.
FAU - Lee, Hong Jun
AU  - Lee HJ
AD  - College of Medicine, Chung Buk National University, Cheongju, Chung Buk 28644, 
      Republic of Korea.
AD  - Research Institute, eBiogen Inc., Seoul, Republic of Korea.
FAU - Cha, Sang-Hoon
AU  - Cha SH
AD  - Department of Radiology, Chung Buk National University Hospital, Cheongju, Chung 
      Buk, Republic of Korea.
AD  - College of Medicine, Chung Buk National University, Cheongju, Chung Buk 28644, 
      Republic of Korea.
FAU - Kim, Hong Nam
AU  - Kim HN
AUID- ORCID: 0000-0002-0329-0029
AD  - Brain Science Institute, Korea Institute of Science and Technology (KIST), Seoul 
      02792, Republic of Korea.
AD  - Division of Bio-Medical Science and Technology, KIST School, Korea University of 
      Science and Technology (UST), Seoul 02792, Republic of Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210505
PL  - England
TA  - Biofabrication
JT  - Biofabrication
JID - 101521964
SB  - IM
MH  - Astrocytes
MH  - *Blood-Brain Barrier
MH  - *Endothelial Cells
MH  - Humans
MH  - *Inflammation
MH  - *Neurons
MH  - Pericytes
OTO - NOTNLM
OT  - blood-brain barrier
OT  - co-culture
OT  - in vitro model
OT  - neuroinflammation
OT  - neurovascular unit
OT  - vascular permeability
EDAT- 2021/04/14 06:00
MHDA- 2021/10/26 06:00
CRDT- 2021/04/13 20:30
PHST- 2020/12/01 00:00 [received]
PHST- 2021/04/13 00:00 [accepted]
PHST- 2021/04/14 06:00 [pubmed]
PHST- 2021/10/26 06:00 [medline]
PHST- 2021/04/13 20:30 [entrez]
AID - 10.1088/1758-5090/abf741 [doi]
PST - epublish
SO  - Biofabrication. 2021 May 5;13(3). doi: 10.1088/1758-5090/abf741.