PMID- 33849116
OWN - NLM
STAT- MEDLINE
DCOM- 20210514
LR  - 20211204
IS  - 2224-5839 (Electronic)
IS  - 2224-5820 (Linking)
VI  - 10
IP  - 3
DP  - 2021 Mar
TI  - Network pharmacology study on the mechanism of the herb pair of prepared 
      Rehmannia root-Chinese arborvitae kernel for anxiety disorders.
PG  - 3313-3327
LID - 10.21037/apm-21-531 [doi]
AB  - BACKGROUND: Although anxiety disorders are one of the most common mental illness 
      in population, antianxiety drugs often only have single action targets, require 
      long-term use, and are associated with many adverse reactions and dependencies. 
      Professor Yan Zhaojun from Shandong Provincial Hospital of Traditional Chinese 
      Medicine (TCM) has applied the modified Renshu Powder, a TCM formula, to treat 
      anxiety disorders, with satisfactory outcomes. Here, we investigated the 
      mechanism of action of two core herbs (prepared Rehmannia root and Chinese 
      arborvitae kernel) in the Renshu Powder in the treatment of anxiety disorders by 
      using network pharmacology approaches. METHODS: Candidate compounds of the herb 
      pair of prepared Rehmannia root-Chinese arborvitae kernel were extracted via the 
      Traditional Chinese Medicine Systems Pharmacology (TCMSP) platform. The targets 
      of action of the main compounds were collected using the SwissTargetPrediction 
      database. Targets associated with anxiety disorders were retrieved from DisGeNET, 
      Online Mendelian Inheritance in Man (OMIM), DrugBank, GeneCards, and Comparative 
      Toxicogenomics Database (CTD) databases. The compound-target interaction network 
      was constructed by Cytoscape 3.7.2 software, and the protein-protein interaction 
      (PPI) network was constructed using the Search Tool for the Retrieval of 
      Interacting Genes/Proteins (STRING) platform. Gene Ontology (GO) and Kyoto 
      Encyclopedia of Genes and Genomes (KEGG) analyses the data by using Metascape. 
      RESULTS: The main active compounds of the herb pair included arachidonic acid, 
      stigmasterol, and beta-sitosterol. The key targets included Nitric Oxide Synthase 
      3 (NOS3), Epidermal growth factor (EGF), Prostaglandin-Endoperoxide Synthase 2 
      (PTGS2), Caspase 3 (CASP3), Mitogen-Activated Protein Kinase 1 (MAPK1), 
      Peroxisome proliferator-activated receptor gamma (PPARG), RELA Proto-Oncogene, 
      NF-KB Subunit (RELA), Estrogen Receptor 1 (ESR1), Solute Carrier Family 6 Member 
      4 (SLC6A4), and Phosphatase and Tensin homolog deleted on chromosome 10 (PTEN). 
      Anxiety disorder-related GO analysis mainly involved synaptic signaling, 
      neurotransmitter receptor activity, and G protein-coupled neurotransmitter 
      receptor activity. The KEGG pathways involved neuroactive ligand-receptor 
      interaction, serotonergic synapse, PI3K/AKT/mTOR signaling pathway, and MAPK 
      signaling pathway. CONCLUSIONS: The mechanism of action of the prepared Rehmannia 
      root-Chinese arborvitae kernel in treating anxiety disorders involves multiple 
      ingredients, multiple targets, and pathways.
FAU - Liu, Pu-Lin
AU  - Liu PL
AD  - College of Traditional Chinese Medicine, Shandong University of Traditional 
      Chinese Medicine, Jinan, China.
FAU - Song, An-Ran
AU  - Song AR
AD  - College of Traditional Chinese Medicine, Shandong University of Traditional 
      Chinese Medicine, Jinan, China.
FAU - Dong, Cheng-Da
AU  - Dong CD
AD  - College of Traditional Chinese Medicine, Shandong University of Traditional 
      Chinese Medicine, Jinan, China.
FAU - Chu, Qian
AU  - Chu Q
AD  - College of Traditional Chinese Medicine, Shandong University of Traditional 
      Chinese Medicine, Jinan, China.
FAU - Xu, Bao-Lin
AU  - Xu BL
AD  - College of Traditional Chinese Medicine, Shandong University of Traditional 
      Chinese Medicine, Jinan, China.
FAU - Liu, Jian-Min
AU  - Liu JM
AD  - Department of Psychosomatic Medicine, Affiliated Hospital of Shandong University 
      of Traditional Chinese Medicine, Jinan, China.
FAU - Yan, Zhao-Jun
AU  - Yan ZJ
AD  - Department of Psychosomatic Medicine, Affiliated Hospital of Shandong University 
      of Traditional Chinese Medicine, Jinan, China. 361257818@163.com.
LA  - eng
PT  - Journal Article
PL  - China
TA  - Ann Palliat Med
JT  - Annals of palliative medicine
JID - 101585484
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (MAS1 protein, human)
RN  - 0 (Proto-Oncogene Mas)
RN  - 0 (SLC6A4 protein, human)
RN  - 0 (Serotonin Plasma Membrane Transport Proteins)
SB  - IM
MH  - Anxiety Disorders/drug therapy/genetics
MH  - China
MH  - *Drugs, Chinese Herbal/therapeutic use
MH  - Humans
MH  - Phosphatidylinositol 3-Kinases
MH  - Proto-Oncogene Mas
MH  - *Rehmannia
MH  - Serotonin Plasma Membrane Transport Proteins
MH  - *Thuja
OTO - NOTNLM
OT  - Chinese arborvitae kernel
OT  - Prepared Rehmannia root
OT  - anxiety disorders
OT  - mechanism of action
OT  - network pharmacology
EDAT- 2021/04/15 06:00
MHDA- 2021/05/15 06:00
CRDT- 2021/04/14 01:00
PHST- 2021/01/27 00:00 [received]
PHST- 2021/03/23 00:00 [accepted]
PHST- 2021/04/14 01:00 [entrez]
PHST- 2021/04/15 06:00 [pubmed]
PHST- 2021/05/15 06:00 [medline]
AID - 10.21037/apm-21-531 [doi]
PST - ppublish
SO  - Ann Palliat Med. 2021 Mar;10(3):3313-3327. doi: 10.21037/apm-21-531.