PMID- 33850541
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210416
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 21
IP  - 6
DP  - 2021 Jun
TI  - MicroRNA-301a-3p increases oxidative stress, inflammation and apoptosis in 
      ox-LDL-induced HUVECs by targeting KLF7.
PG  - 569
LID - 10.3892/etm.2021.10001 [doi]
LID - 569
AB  - Arteriosclerotic cardiovascular disease is an inflammatory disease of ischemia or 
      endothelial dysfunction caused by atherosclerosis, thereby causing high 
      mortality. The viability and apoptosis of human umbilical vein endothelial cells 
      (HUVECs) following oxidized low-density lipoprotein (ox-LDL) induction or 
      transfection was detected by Cell Counting Kit-8 (CCK-8) assay and flow cytometry 
      analysis. MicroRNA (miR)-301a-3p and Krueppel-like factor 7 (KLF7) mRNA 
      expression was determined by reverse transcription-quantitative PCR (RT-qPCR). 
      The levels of monocyte chemoattractant protein-1 (MCP-1) and IL-6, activities of 
      reactive oxygen species and superoxide dismutase and lactate dehydrogenase 
      leakage were analyzed by respective commercial assay kits. The protein expression 
      of IL-6, MCP-1, Bcl2, Bax, poly (ADP-ribose) polymerase (PARP), cleaved PARP, 
      pro-caspase3 and cleaved caspase-3 was detected by western blotting. miR-301a-3p 
      expression is highly expressed in ox-LDL-induced HUVECs. miR-301a-3p is also a 
      target of KLF7. Inhibition of miR-301a-3p suppressed oxidative stress, 
      inflammation and apoptosis in ox-LDL-induced HUVECs, which was reversed by KLF7 
      inhibition. In conclusion, miR-301a-3p promotes oxidative stress, inflammation 
      and apoptosis in ox-LDL-induced HUVECs via decreasing KLF7 expression.
CI  - Copyright: (c) Jiang et al.
FAU - Jiang, Huiqiong
AU  - Jiang H
AD  - Cardiac Function Examination Room, Quanzhou First Hospital, Quanzhou, Fujian 
      362000, P.R. China.
FAU - Lv, Jiaren
AU  - Lv J
AD  - Cardiac Function Examination Room, Quanzhou First Hospital, Quanzhou, Fujian 
      362000, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20210329
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC8027757
OTO - NOTNLM
OT  - apoptosis
OT  - inflammation
OT  - microRNA-301a-3p
OT  - oxidative stress
OT  - oxidized low-density lipoprotein-induced human umbilical vein endothelial cells
COIS- The authors declare they have no competing interests.
EDAT- 2021/04/15 06:00
MHDA- 2021/04/15 06:01
PMCR- 2021/03/29
CRDT- 2021/04/14 06:32
PHST- 2020/09/22 00:00 [received]
PHST- 2021/01/08 00:00 [accepted]
PHST- 2021/04/14 06:32 [entrez]
PHST- 2021/04/15 06:00 [pubmed]
PHST- 2021/04/15 06:01 [medline]
PHST- 2021/03/29 00:00 [pmc-release]
AID - ETM-0-0-10001 [pii]
AID - 10.3892/etm.2021.10001 [doi]
PST - ppublish
SO  - Exp Ther Med. 2021 Jun;21(6):569. doi: 10.3892/etm.2021.10001. Epub 2021 Mar 29.