PMID- 33850556
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210416
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 21
IP  - 6
DP  - 2021 Jun
TI  - Diclofenac impairs the proliferation and glucose metabolism of triple-negative 
      breast cancer cells by targeting the c-Myc pathway.
PG  - 584
LID - 10.3892/etm.2021.10016 [doi]
LID - 584
AB  - Triple-negative breast cancer (TNBC) cells obtain energy mainly through aerobic 
      glycolysis, and their glycolytic rate is significantly higher compared with that 
      of non-TNBC cells. Glucose transporter 1 (GLUT1) is a transmembrane transporter 
      necessary for the entry of glucose into tumor cells, hexokinase (HK) is a key 
      enzyme in the glycolytic pathway, and both are targets of the transcription 
      factor c-Myc. c-Myc can promote aerobic glycolysis by upregulating GLUT1 
      expression and enhancing HK activity. c-Myc and GLUT1 are highly expressed in 
      TNBC. The non-steroidal anti-inflammatory drug diclofenac can inhibit glycolysis 
      in melanoma cells and thereby promote apoptosis by downregulating c-Myc and 
      GLUT1. To explore the effect of diclofenac on the energy metabolism of TNBC cells 
      and determine the underlying mechanism, a comparative study in two TNBC cell 
      lines (MDA-MB-231 and HCC1937) and one non-TNBC cell line (MCF-7) was conducted. 
      Cell proliferation was detected by Cell Counting Kit-8 (CCK-8) and flow 
      cytometric assays; GLUT1 and c-Myc expression was measured by western blotting. 
      Diclofenac significantly inhibited cell proliferation, downregulated GLUT1 and 
      c-Myc expression, and decreased HK activity in TNBC cells compared with non-TNBC 
      cells. In conclusion, the studies suggested that diclofenac inhibited cell 
      glycolysis and suppressed TNBC cell growth by decreasing GLUT1 protein expression 
      and HK activity through the c-Myc pathway.
CI  - Copyright: (c) Yang et al.
FAU - Yang, Lihui
AU  - Yang L
AD  - Department of Nursing, Guangxi Medical University Nursing College, Nanning, 
      Guangxi 530021, P.R. China.
FAU - Li, Jiachen
AU  - Li J
AD  - Department of Clinical Medicine, Guangxi Medical University The First Clinical 
      Medical College, Nanning, Guangxi 530021, P.R. China.
FAU - Li, Yongzhuo
AU  - Li Y
AD  - Department of Medicine Guangxi University Medical College, Nanning, Guangxi 
      530004, P.R. China.
FAU - Zhou, Yongli
AU  - Zhou Y
AD  - Department of Clinical Medicine, Guangxi Medical University The First Clinical 
      Medical College, Nanning, Guangxi 530021, P.R. China.
FAU - Wang, Ziqian
AU  - Wang Z
AD  - Department of Clinical Medicine, Guangxi Medical University The First Clinical 
      Medical College, Nanning, Guangxi 530021, P.R. China.
FAU - Zhang, Dahao
AU  - Zhang D
AD  - Department of Clinical Medicine, Guangxi Medical University The First Clinical 
      Medical College, Nanning, Guangxi 530021, P.R. China.
FAU - Liu, Jinlu
AU  - Liu J
AD  - Department of Gastrointestinal and Gland Surgery, The First Affiliated Hospital 
      of Guangxi Medical University, Nanning, Guangxi 530021, P.R. China.
FAU - Zhang, Xiaodong
AU  - Zhang X
AD  - Department of Gastrointestinal and Gland Surgery, The First Affiliated Hospital 
      of Guangxi Medical University, Nanning, Guangxi 530021, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20210402
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC8027724
OTO - NOTNLM
OT  - GLUT1
OT  - c-Myc
OT  - diclofenac
OT  - glycolysis
OT  - triple-negative breast cancer
COIS- The authors declare that they have no competing interests.
EDAT- 2021/04/15 06:00
MHDA- 2021/04/15 06:01
PMCR- 2021/04/02
CRDT- 2021/04/14 06:32
PHST- 2020/06/17 00:00 [received]
PHST- 2021/02/26 00:00 [accepted]
PHST- 2021/04/14 06:32 [entrez]
PHST- 2021/04/15 06:00 [pubmed]
PHST- 2021/04/15 06:01 [medline]
PHST- 2021/04/02 00:00 [pmc-release]
AID - ETM-0-0-10016 [pii]
AID - 10.3892/etm.2021.10016 [doi]
PST - ppublish
SO  - Exp Ther Med. 2021 Jun;21(6):584. doi: 10.3892/etm.2021.10016. Epub 2021 Apr 2.