PMID- 33851807
OWN - NLM
STAT- MEDLINE
DCOM- 20210628
LR  - 20220531
IS  - 1897-9483 (Electronic)
IS  - 0032-3772 (Linking)
VI  - 131
IP  - 5
DP  - 2021 May 25
TI  - Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase p22phox subunit 
      polymorphisms, systemic oxidative stress, endothelial dysfunction, and 
      atherosclerosis in type 2 diabetes mellitus.
PG  - 447-454
LID - 10.20452/pamw.15937 [doi]
AB  - INTRODUCTION: Diabetes mellitus is an important and rapidly increasing problem in 
      public health. It associates with endothelial dysfunction and increased 
      endothelial permeability, which may lead to severe cardiovascular events. 
      OBJECTIVES: We aimed to evaluate the relationship between polymorphisms in the 
      cytochrome b-245 alpha chain (CYBA) gene encoding p22phox, a key subunit of 
      nicotinamide adenine dinucleotide phosphate (NADPH) oxidase, with endothelial 
      function, atherosclerosis and systemic oxidative stress in type 2 diabetes 
      mellitus (T2DM). PATIENTS AND METHODS: Intima-media thickness (IMT), flow- and 
      nitroglycerin-mediated dilatation (FMD and NMD) were measured in 182 patients 
      with T2DM. Assessment of plasma levels of von Willebrand factor (vWF) and 
      malondialdehyde (MDA) levels as well as genotyping of coding sequence C242T 
      (rs4673) and promoter region A-930G (rs9932581) polymorphisms of CYBA were 
      performed using standardized protocols. RESULTS: We observed a significant 
      association of the impaired endothelial function, as measured by FMD, with the C 
      allele of C242T, but not with the A-930G polymorphism. Functional relationship of 
      the C242T polymorphism with endothelial dysfunction remained significant 
      following a multivariable adjustment for major risk factors for atherosclerosis. 
      Mean IMT, NMD, concentrations of MDA or vWF were not related to the specific 
      genotypes of the investigated polymorphisms. CONCLUSIONS: C242T, but not A-930G, 
      polymorphism of CYBA significantly affects endothelial function in T2DM. Thus, it 
      might be a useful marker of endothelial dysfunction in T2DM patients.
FAU - Osmenda, Grzegorz
AU  - Osmenda G
AD  - Department of Internal and Agricultural Medicine, Faculty of Medicine, 
      Jagiellonian University Medical College, Krakow, Poland
FAU - Matusik, Pawel T
AU  - Matusik PT
AD  - Department of Internal and Agricultural Medicine, Faculty of Medicine, 
      Jagiellonian University Medical College, Krakow, Poland
AD  - Department of Electrocardiology, Institute of Cardiology, Faculty of Medicine, 
      Jagiellonian University Medical College, Krakow, Poland
FAU - Sliwa, Tomasz
AU  - Sliwa T
AD  - Department of Internal and Agricultural Medicine, Faculty of Medicine, 
      Jagiellonian University Medical College, Krakow, Poland
FAU - Czesnikiewicz-Guzik, Marta
AU  - Czesnikiewicz-Guzik M
AD  - Department of Periodontology and Oral Sciences Research Group, University of 
      Glasgow Dental School and Institute of Infection, Immunity and Inflammation, 
      University of Glasgow, Glasgow, United Kingdom
AD  - Department of Dental Prophylaxis and Experimental Dentistry, Faculty of Medicine, 
      Jagiellonian University Medical College, Krakow, Poland
FAU - Skupien, Jan
AU  - Skupien J
AD  - Department of Metabolic Diseases, Faculty of Medicine, Jagiellonian University 
      Medical College, Krakow, Poland
FAU - Malecki, Maciej T
AU  - Malecki MT
AD  - Department of Metabolic Diseases, Faculty of Medicine, Jagiellonian University 
      Medical College, Krakow, Poland
FAU - Siedlinski, Mateusz
AU  - Siedlinski M
AD  - Department of Internal and Agricultural Medicine, Faculty of Medicine, 
      Jagiellonian University Medical College, Krakow, Poland. 
      mateusz.siedlinski@uj.edu.pl
LA  - eng
PT  - Journal Article
DEP - 20210414
PL  - Poland
TA  - Pol Arch Intern Med
JT  - Polish archives of internal medicine
JID - 101700960
RN  - 53-59-8 (NADP)
RN  - EC 1.6.3.- (NADPH Oxidases)
RN  - EC 1.6.3.1 (CYBA protein, human)
SB  - IM
MH  - *Atherosclerosis/genetics
MH  - Carotid Intima-Media Thickness
MH  - *Diabetes Mellitus, Type 2/complications/genetics
MH  - Humans
MH  - NADP
MH  - NADPH Oxidases/genetics/metabolism
MH  - Oxidative Stress/genetics
EDAT- 2021/04/15 06:00
MHDA- 2021/06/29 06:00
CRDT- 2021/04/14 09:12
PHST- 2021/04/15 06:00 [pubmed]
PHST- 2021/06/29 06:00 [medline]
PHST- 2021/04/14 09:12 [entrez]
AID - 10.20452/pamw.15937 [doi]
PST - ppublish
SO  - Pol Arch Intern Med. 2021 May 25;131(5):447-454. doi: 10.20452/pamw.15937. Epub 
      2021 Apr 14.