PMID- 33860259
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210417
IS  - 0022-300X (Print)
IS  - 2640-396X (Electronic)
IS  - 0022-300X (Linking)
VI  - 53
DP  - 2021
TI  - First report of morphological and molecular characterization of Moroccan 
      populations of Globodera pallida.
LID - 10.21307/jofnem-2021-007 [doi]
LID - e2021-07
AB  - Potato cyst nematodes (PCNs) are the most important potato pest causing major 
      crop losses across the world with a quarantine status in many countries. In 
      Morocco, several potato crops are infected with PCNs and the monitoring of potato 
      production as well as the control of import and export of potato seeds are 
      currently carried out by morphological methods. The present work was aimed to use 
      molecular and morphometric methods for identifying and differentiating PCN 
      species in Morocco for the first time. The morphological identification of PCN 
      species from collected soil samples were carried out using the shape of the 
      cysts, the length of the stylet, the number of cuticular ridges, and the Granek's 
      ratio. The J2 had a slightly shorter body length, the number of cuticular ridges 
      was 9 and the Granek's ratio averaged 2.2. The morphobiometric analysis revealed 
      proximity of the Moroccan population to G. pallida species. PCNs sampled from 
      contaminated fields were analyzed molecularly using PCR. DNA amplification was 
      performed using the multiplex PCR method and PCR-RFLP from the ITS region of the 
      total genomic DNA compared to multiplex PCR-specific DNA sequences. All confirmed 
      the presence G. pallida in all samples of the Moroccan PCN populations.
CI  - (c) 2021 Authors.
FAU - Hajjaji, A
AU  - Hajjaji A
AD  - Research Unit Microbiology, Hygiene and Biomolecules, Laboratory of Virology, 
      Microbiology, Quality and Biotechnology/Ecotoxicology and Biodiversity, 
      University Hassan II of Casablanca, FSTM, Casablanca, Morocco.
AD  - Plant Quarantine Laboratory, Bouznika, Morocco.
FAU - Mhand, R Ait
AU  - Mhand RA
AD  - Research Unit Microbiology, Hygiene and Biomolecules, Laboratory of Virology, 
      Microbiology, Quality and Biotechnology/Ecotoxicology and Biodiversity, 
      University Hassan II of Casablanca, FSTM, Casablanca, Morocco.
FAU - Rhallabi, N
AU  - Rhallabi N
AD  - Research Unit Microbiology, Hygiene and Biomolecules, Laboratory of Virology, 
      Microbiology, Quality and Biotechnology/Ecotoxicology and Biodiversity, 
      University Hassan II of Casablanca, FSTM, Casablanca, Morocco.
FAU - Mellouki, F
AU  - Mellouki F
AD  - Research Unit Microbiology, Hygiene and Biomolecules, Laboratory of Virology, 
      Microbiology, Quality and Biotechnology/Ecotoxicology and Biodiversity, 
      University Hassan II of Casablanca, FSTM, Casablanca, Morocco.
LA  - eng
PT  - Journal Article
DEP - 20210225
PL  - Poland
TA  - J Nematol
JT  - Journal of nematology
JID - 9884299
PMC - PMC8039999
OTO - NOTNLM
OT  - Cyst nematodes
OT  - Globodera pallida
OT  - Globodera rostochiensis
OT  - Molecular-identification
OT  - Morocco
OT  - Potato
OT  - RFLP
EDAT- 2021/04/17 06:00
MHDA- 2021/04/17 06:01
PMCR- 2021/02/25
CRDT- 2021/04/16 06:42
PHST- 2020/10/15 00:00 [received]
PHST- 2021/04/16 06:42 [entrez]
PHST- 2021/04/17 06:00 [pubmed]
PHST- 2021/04/17 06:01 [medline]
PHST- 2021/02/25 00:00 [pmc-release]
AID - e2021-007 [pii]
AID - 10.21307/jofnem-2021-007 [doi]
PST - epublish
SO  - J Nematol. 2021 Feb 25;53:e2021-07. doi: 10.21307/jofnem-2021-007. eCollection 
      2021.