PMID- 33861159 OWN - NLM STAT- MEDLINE DCOM- 20211025 LR - 20220417 IS - 2164-554X (Electronic) IS - 2164-5515 (Print) IS - 2164-5515 (Linking) VI - 17 IP - 9 DP - 2021 Sep 2 TI - The dynamics and association of B and T cell receptor repertoires upon antibody response to hepatitis B vaccination in healthy adults. PG - 3203-3213 LID - 10.1080/21645515.2021.1913028 [doi] AB - The Hepatitis B (HB) vaccine is efficacious in preventing hepatitis B virus infection. However, the association between antibody response to the HB vaccine and dynamic immune repertoire changes in different cell subsets remains unclear. Nine healthy participants were administered three doses of HB vaccine following the 0, 1, 6 month schedule. Peripheral CD4(+) T, memory B (MB), naive B (NB), and plasma cells (PCs) were sorted before vaccination and 7 days following each dose. The complementary determining region 3 of T-cell receptor beta (TCRbeta) chain and B-cell receptor (BCR) heavy chain (IgG, IgM, IgA) repertoires were analyzed by high-throughput sequencing. All nine participants elicited protective antibody titers to the vaccine at the end of immunization. Compared with the baseline, MB cells showed a significant increase in IgG usage and decreased IgM usage and repertoire diversity at the end of vaccination. TCRbeta diversity changes were highly correlated with those of the BCR in MB cells in participants with a faster and robust antibody responses. The percentage of shared clonotypes between NB and MB cells, and MB cells and PCs were much higher than that between NB cells and PCs. The more clonotypes sharing the faster and stronger antibody responses were observed after HB vaccination. These results suggest the integral involvement of MB cells in vaccine immunization. Interaction between CD4(+) T and MB cells and B cell differentiation may improve antibody response to HB vaccine. FAU - Zhao, Miaoxian AU - Zhao M AD - State Key Laboratory of Organ Failure Research, Guangdong Provincial Key Laboratory of Viral Hepatitis Research, Department of Infectious Diseases and Hepatology Unit, Nanfang Hospital, Southern Medical University, Guangzhou, China. FAU - Li, Xueying AU - Li X AD - State Key Laboratory of Organ Failure Research, Guangdong Provincial Key Laboratory of Viral Hepatitis Research, Department of Infectious Diseases and Hepatology Unit, Nanfang Hospital, Southern Medical University, Guangzhou, China. FAU - Xie, Shi AU - Xie S AD - State Key Laboratory of Organ Failure Research, Guangdong Provincial Key Laboratory of Viral Hepatitis Research, Department of Infectious Diseases and Hepatology Unit, Nanfang Hospital, Southern Medical University, Guangzhou, China. FAU - Gong, Mingxing AU - Gong M AUID- ORCID: 0000-0002-6506-8706 AD - State Key Laboratory of Organ Failure Research, Guangdong Provincial Key Laboratory of Viral Hepatitis Research, Department of Infectious Diseases and Hepatology Unit, Nanfang Hospital, Southern Medical University, Guangzhou, China. FAU - Yan, Rong AU - Yan R AD - State Key Laboratory of Organ Failure Research, Guangdong Provincial Key Laboratory of Viral Hepatitis Research, Department of Infectious Diseases and Hepatology Unit, Nanfang Hospital, Southern Medical University, Guangzhou, China. FAU - Zheng, Anqi AU - Zheng A AD - State Key Laboratory of Organ Failure Research, Guangdong Provincial Key Laboratory of Viral Hepatitis Research, Department of Infectious Diseases and Hepatology Unit, Nanfang Hospital, Southern Medical University, Guangzhou, China. FAU - Xu, Ying AU - Xu Y AUID- ORCID: 0000-0003-1452-8423 AD - State Key Laboratory of Organ Failure Research, Guangdong Provincial Key Laboratory of Viral Hepatitis Research, Department of Infectious Diseases and Hepatology Unit, Nanfang Hospital, Southern Medical University, Guangzhou, China. FAU - Wu, Hongkai AU - Wu H AUID- ORCID: 0000-0003-3787-8098 AD - State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, China. FAU - Wang, Zhanhui AU - Wang Z AUID- ORCID: 0000-0001-9022-6545 AD - State Key Laboratory of Organ Failure Research, Guangdong Provincial Key Laboratory of Viral Hepatitis Research, Department of Infectious Diseases and Hepatology Unit, Nanfang Hospital, Southern Medical University, Guangzhou, China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20210416 PL - United States TA - Hum Vaccin Immunother JT - Human vaccines & immunotherapeutics JID - 101572652 RN - 0 (Hepatitis B Antibodies) RN - 0 (Hepatitis B Vaccines) RN - 0 (Receptors, Antigen, T-Cell, alpha-beta) SB - IM MH - Adult MH - *Antibody Formation MH - *Hepatitis B/prevention & control MH - Hepatitis B Antibodies MH - Hepatitis B Vaccines MH - Humans MH - Receptors, Antigen, T-Cell, alpha-beta MH - Vaccination PMC - PMC8381827 OTO - NOTNLM OT - B cell receptor OT - Hepatitis B vaccine OT - T cell receptor OT - antibody response OT - repertoire EDAT- 2021/04/17 06:00 MHDA- 2021/10/26 06:00 PMCR- 2022/04/16 CRDT- 2021/04/16 12:10 PHST- 2021/04/17 06:00 [pubmed] PHST- 2021/10/26 06:00 [medline] PHST- 2021/04/16 12:10 [entrez] PHST- 2022/04/16 00:00 [pmc-release] AID - 1913028 [pii] AID - 10.1080/21645515.2021.1913028 [doi] PST - ppublish SO - Hum Vaccin Immunother. 2021 Sep 2;17(9):3203-3213. doi: 10.1080/21645515.2021.1913028. Epub 2021 Apr 16.