PMID- 33863453
OWN - NLM
STAT- MEDLINE
DCOM- 20211112
LR  - 20211214
IS  - 0306-4565 (Print)
IS  - 0306-4565 (Linking)
VI  - 97
DP  - 2021 Apr
TI  - The effect of Mitoquinol (MitoQ) on heat stressed skeletal muscle from pigs, and 
      a potential confounding effect of biological sex.
PG  - 102900
LID - S0306-4565(21)00067-X [pii]
LID - 10.1016/j.jtherbio.2021.102900 [doi]
AB  - Heat stress (HS) poses a major threat to human health and agricultural 
      production. Oxidative stress and mitochondrial dysfunction appear to play key 
      roles in muscle injury caused by HS. We hypothesized that mitoquinol (MitoQ), 
      would alleviate oxidative stress and cellular dysfunction in skeletal muscle 
      during HS. To address this, crossbred barrows (male pigs) were treated with 
      placebo or MitoQ (40 mg/d) and were then exposed to thermoneutral (TN; 20  degrees C) or 
      HS (35  degrees C) conditions for 24 h. Pigs were euthanized following the environmental 
      challenge and the red portion of the semitendinosus (STR) was collected for 
      analysis. Unexpectedly, malondialdehyde concentration, an oxidative stress 
      marker, was similar between environmental and supplement treatments. Heat stress 
      decreased LC3A/B-I (p < 0.05) and increased the ratio of LC3A/B-II/I (p < 0.05), 
      while p62 was similar among groups suggesting increased degradation of 
      autophagosomes during HS. These outcomes were in disagreement with our previous 
      results in muscle from gilts (female pigs). To probe the impact of biological sex 
      on HS-mediated injury in skeletal muscle, we compared STR from these barrows to 
      archived STR from gilts subjected to a similar environmental intervention. We 
      confirmed our previous findings of HS-mediated dysfunction in muscle from gilts 
      but not barrows. These data also raise the possibility that muscle from gilts is 
      more susceptible to environment-induced hyperthermia than muscle from barrows.
CI  - Copyright (c) 2021. Published by Elsevier Ltd.
FAU - Rudolph, Tori E
AU  - Rudolph TE
AD  - Department of Animal Science, Iowa State University, Ames, IA, USA.
FAU - Mayorga, Edith J
AU  - Mayorga EJ
AD  - Department of Animal Science, Iowa State University, Ames, IA, USA.
FAU - Roths, Melissa
AU  - Roths M
AD  - Department of Animal Science, Iowa State University, Ames, IA, USA.
FAU - Rhoads, Robert P
AU  - Rhoads RP
AD  - Department of Animal and Poultry Sciences, Virginia Tech, Blacksburg, VA, USA.
FAU - Baumgard, Lance H
AU  - Baumgard LH
AD  - Department of Animal Science, Iowa State University, Ames, IA, USA.
FAU - Selsby, Joshua T
AU  - Selsby JT
AD  - Department of Animal Science, Iowa State University, Ames, IA, USA. Electronic 
      address: jselsby@iastate.edu.
LA  - eng
PT  - Journal Article
PT  - Randomized Controlled Trial, Veterinary
DEP - 20210306
PL  - England
TA  - J Therm Biol
JT  - Journal of thermal biology
JID - 7600115
RN  - 0 (Antioxidants)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (Organophosphorus Compounds)
RN  - 1339-63-5 (Ubiquinone)
RN  - 47BYS17IY0 (mitoquinone)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - 65M41NIO61 (mitoquinol)
SB  - IM
MH  - Animals
MH  - Antioxidants/*pharmacology
MH  - Autophagy/drug effects
MH  - Female
MH  - Heat-Shock Response/*drug effects
MH  - Male
MH  - Malondialdehyde/metabolism
MH  - Microtubule-Associated Proteins/metabolism
MH  - Muscle, Skeletal/*drug effects/metabolism
MH  - Organophosphorus Compounds/*pharmacology
MH  - Oxidative Stress/drug effects
MH  - *Sex Characteristics
MH  - Swine
MH  - Ubiquinone/*analogs & derivatives/pharmacology
OTO - NOTNLM
OT  - Autophagy
OT  - Hyperthermia
OT  - MitoQ
OT  - Mitochondria
OT  - Oxidative stress
EDAT- 2021/04/18 06:00
MHDA- 2021/11/16 06:00
CRDT- 2021/04/17 05:30
PHST- 2020/11/16 00:00 [received]
PHST- 2021/02/12 00:00 [revised]
PHST- 2021/03/01 00:00 [accepted]
PHST- 2021/04/17 05:30 [entrez]
PHST- 2021/04/18 06:00 [pubmed]
PHST- 2021/11/16 06:00 [medline]
AID - S0306-4565(21)00067-X [pii]
AID - 10.1016/j.jtherbio.2021.102900 [doi]
PST - ppublish
SO  - J Therm Biol. 2021 Apr;97:102900. doi: 10.1016/j.jtherbio.2021.102900. Epub 2021 
      Mar 6.