PMID- 33864661
OWN - NLM
STAT- MEDLINE
DCOM- 20211108
LR  - 20211108
IS  - 1791-2431 (Electronic)
IS  - 1021-335X (Print)
IS  - 1021-335X (Linking)
VI  - 45
IP  - 5
DP  - 2021 May
TI  - Knockdown of PNO1 inhibits esophageal cancer progression.
LID - 85 [pii]
LID - 10.3892/or.2021.8036 [doi]
AB  - The present study aimed to investigate the role of partner of NOB1 homolog (PNO1) 
      in esophageal cancer (EC). The expression levels of PNO1 in EC were primarily 
      analyzed using data obtained from databases. PNO1 expression was also knocked 
      down in EC cells (Eca‑109 and TE1) to determine the biological effects of PNO1 on 
      tumorigenesis in vitro and in vivo. In addition, possible downstream targets of 
      PNO1 in EC were identified. The expression levels of PNO1 were upregulated in the 
      tumor tissues compared with that noted in normal tissues. Moreover, the knockdown 
      (KD) of PNO1 suppressed cell proliferation, migration and invasion, and promoted 
      cell apoptosis (P < 0.05). Furthermore, the protein expression levels of AKT1, 
      Twist, Myc, mTOR, matrix metalloproteinase 2 (MMP2), nuclear factor (NF)‑kappaB p65 
      and beta‑catenin 1 (CTNNB1) were downregulated following the KD of PNO1 in Eca‑109 
      cells (P < 0.05). In addition, the overexpression of CTNNB1 reversed the effects 
      of PNO1 KD in Eca‑109 cells (P < 0.05). In conclusion, the findings of the 
      present study suggest that PNO1 promotes EC progression by regulating AKT1, 
      Twist, Myc, mTOR, MMP2, NF‑kappaB p65 and CTNNB1 expression.
FAU - Wang, Guowen
AU  - Wang G
AD  - Department of Thoracic Surgery, The First Affiliated Hospital of Bengbu Medical 
      College, Bengbu, Anhui 233030, P.R. China.
FAU - Li, Qicai
AU  - Li Q
AD  - Department of Thoracic Surgery, The First Affiliated Hospital of Bengbu Medical 
      College, Bengbu, Anhui 233030, P.R. China.
FAU - Li, Chuankui
AU  - Li C
AD  - Department of Thoracic Surgery, The First Affiliated Hospital of Bengbu Medical 
      College, Bengbu, Anhui 233030, P.R. China.
FAU - Duan, Guixin
AU  - Duan G
AD  - Department of Thoracic Surgery, The First Affiliated Hospital of Bengbu Medical 
      College, Bengbu, Anhui 233030, P.R. China.
FAU - Sang, Haiwei
AU  - Sang H
AD  - Department of Thoracic Surgery, The First Affiliated Hospital of Bengbu Medical 
      College, Bengbu, Anhui 233030, P.R. China.
FAU - Dong, Haijun
AU  - Dong H
AD  - Department of Thoracic Surgery, Huzhou Central Hospital, Huzhou, Zhejiang 310000, 
      P.R. China.
FAU - Yang, Yifan
AU  - Yang Y
AD  - Department of Thoracic Surgery, The First Affiliated Hospital of Bengbu Medical 
      College, Bengbu, Anhui 233030, P.R. China.
FAU - Ma, Chang
AU  - Ma C
AD  - Department of Thoracic Surgery, The First Affiliated Hospital of Bengbu Medical 
      College, Bengbu, Anhui 233030, P.R. China.
FAU - Tao, Tao
AU  - Tao T
AD  - Department of Thoracic Surgery, The First Affiliated Hospital of Bengbu Medical 
      College, Bengbu, Anhui 233030, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20210413
PL  - Greece
TA  - Oncol Rep
JT  - Oncology reports
JID - 9422756
RN  - 0 (PNO1 protein, human)
RN  - 0 (RNA-Binding Proteins)
SB  - IM
MH  - Animals
MH  - Apoptosis/genetics
MH  - Carcinogenesis/*genetics/pathology
MH  - Cell Line, Tumor
MH  - Cell Proliferation/genetics
MH  - Datasets as Topic
MH  - Disease Progression
MH  - Esophageal Neoplasms/diagnosis/*genetics/pathology
MH  - Esophagus/pathology
MH  - Female
MH  - *Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Mice
MH  - Neoplasm Staging
MH  - RNA-Binding Proteins/genetics/*metabolism
MH  - RNA-Seq
MH  - Up-Regulation
MH  - Xenograft Model Antitumor Assays
PMC - PMC8025143
OTO - NOTNLM
OT  - partner of NOB1 homolog
OT  - CTNNB1
OT  - esophageal cancer
OT  - AKT1
OT  - cell proliferation
COIS- The authors declare that they have no competing interests.
EDAT- 2021/04/18 06:00
MHDA- 2021/11/09 06:00
PMCR- 2021/03/31
CRDT- 2021/04/17 15:08
PHST- 2020/07/23 00:00 [received]
PHST- 2021/03/11 00:00 [accepted]
PHST- 2021/04/17 15:08 [entrez]
PHST- 2021/04/18 06:00 [pubmed]
PHST- 2021/11/09 06:00 [medline]
PHST- 2021/03/31 00:00 [pmc-release]
AID - 85 [pii]
AID - OR-0-0-8036 [pii]
AID - 10.3892/or.2021.8036 [doi]
PST - ppublish
SO  - Oncol Rep. 2021 May;45(5):85. doi: 10.3892/or.2021.8036. Epub 2021 Apr 13.