PMID- 33865300
OWN - NLM
STAT- Publisher
LR  - 20240222
IS  - 0125-877X (Print)
IS  - 0125-877X (Linking)
DP  - 2021 Apr 18
TI  - A novel nested allele-specific PCR protocol for the detection of the HLA-A*33:03, 
      a SCAR-associated allele, in Vietnamese people.
LID - 10.12932/AP-201120-1000 [doi]
AB  - BACKGROUND: Severe cutaneous adverse drug reactions (SCARs) are rare but deadly 
      drug reactions with severe damages to patients. One of the most well-known SCARs 
      risk factors is the human leukocyte antigen (HLA) genes polymorphism. Among the 
      HLA polymorphic alleles, the HLA-A*33:03 allele has been found in association 
      with SCARs induced by various drugs, especially in Asian people. There has not 
      been any report on the specific detection protocol of the HLA-A*33:03 allele. 
      OBJECTIVE: This study aimed to design a nested AS-PCR protocol for detecting and 
      distinguishing diplotype genotype of the HLA-A*33:03 allele. METHODS: A nested 
      allele-specific (AS)-PCR protocol with four primer sets was designed. The method 
      was compared with the Sanger sequencing method on 100 samples of unknown 
      genotypes of unrelated Vietnamese people. RESULTS: The nested AS-PCR method could 
      identify the HLA-A*33:03 allele and the HLA-A*33:03 diplotype genotypes. 
      Comparison with the Sanger sequencing method showed an absolute agreement (kappa = 
      1.00, p < 0.001). The nested ASPCR protocol had a sensitivity of 100% (95%CI: 
      92.13-100%) and a specificity of 100% (95%CI: 93.51-100%). The protocol was used 
      for the determination of HLA-A*33:03 allele distribution in 810 unrelated 
      Vietnamese Kinh people, showing a frequency of HLA-A*33:03 carriers of 19.6% and 
      an allele frequency of 10.55%. CONCLUSIONS: A novel nested AS-PCR method with a 
      hundred-percent sensitivity and a specificity for the HLA-A*33:03 allele 
      detection was reported. The protocol can be applied for the stratification of 
      patients at SCAR risks with various drugs.
FAU - Pham, Tran Thu Ha
AU  - Pham TTH
AD  - Hanoi University of Pharmacy, Hanoi, Vietnam.
FAU - Tran, Quang Binh
AU  - Tran QB
AD  - National Institute of Nutrition, Hanoi, Vietnam.
FAU - Sukasem, Chonlaphat
AU  - Sukasem C
AD  - Division of Pharmacogenomics and Personalized Medicine, Faculty of Medicine, 
      Ramathibodi Hospital, Mahidol University, Thailand.
AD  - Laboratory for Pharmacogenomics, Somdech Phra Debaratana Medical Center (SDMC), 
      Ramathibodi Hospital, Bangkok, Thailand.
AD  - The Thai Severe Cutaneous Adverse Drug Reaction (THAI-SCAR) research group, 
      Thailand.
FAU - Nguyen, Van Dinh
AU  - Nguyen VD
AD  - Allergy and Clinical Immunology Unit, Internal Medicine Department, Vinmec Times 
      City International Hospital, Vinmec Healthcare System, Hanoi, Vietnam.
AD  - College of Health Sciences, VinUniversity, Hanoi, Vietnam.
FAU - Chu, Chi Hieu
AU  - Chu CH
AD  - Center of Allergology and Clinical Immunology, Bach Mai Hospital, Hanoi, Viet 
      Nam.
FAU - Do, Thi Quynh Nga
AU  - Do TQN
AD  - National Institute of Hygiene and Epidemiology, Hanoi, Vietnam.
FAU - Tran, Ngoc Phuong Mai
AU  - Tran NPM
AD  - National Institute of Hygiene and Epidemiology, Hanoi, Vietnam.
FAU - Nguyen, Hai Ha
AU  - Nguyen HH
AD  - Institute of Genome Research, Vietnam Academy of Science and Technology, Hanoi, 
      Vietnam.
FAU - Phung, Thanh Huong
AU  - Phung TH
AD  - Hanoi University of Pharmacy, Hanoi, Vietnam.
LA  - eng
PT  - Journal Article
DEP - 20210418
PL  - Thailand
TA  - Asian Pac J Allergy Immunol
JT  - Asian Pacific journal of allergy and immunology
JID - 8402034
SB  - IM
EDAT- 2021/04/19 06:00
MHDA- 2021/04/19 06:00
CRDT- 2021/04/18 20:19
PHST- 2021/04/18 20:19 [entrez]
PHST- 2021/04/19 06:00 [pubmed]
PHST- 2021/04/19 06:00 [medline]
AID - 10.12932/AP-201120-1000 [doi]
PST - aheadofprint
SO  - Asian Pac J Allergy Immunol. 2021 Apr 18. doi: 10.12932/AP-201120-1000.