PMID- 33865334
OWN - NLM
STAT- MEDLINE
DCOM- 20210430
LR  - 20210430
IS  - 1471-2334 (Electronic)
IS  - 1471-2334 (Linking)
VI  - 21
IP  - 1
DP  - 2021 Apr 17
TI  - Virulence genes and phylogenetic groups of uropathogenic Escherichia coli 
      isolates from patients with urinary tract infection and uninfected control 
      subjects: a case-control study.
PG  - 361
LID - 10.1186/s12879-021-06036-4 [doi]
LID - 361
AB  - BACKGROUND: Urinary Tract Infection (UTI) is one of the most common bacterial 
      infectious diseases which causes considerable morbidity and costly health 
      problems. Uropathogenic Escherichia coli (UPEC), the most common pathogen causing 
      UTI, is a highly heterogeneous group of extraintestinal pathogenic E. coli 
      (ExPEC) which may carry a variety of virulence factors and belonging to different 
      phylogenetic backgrounds. The current study aimed to investigate the frequency 
      and association between various virulence factors (VFs) and phylogenetic groups 
      of UPEC and commensal isolates. METHODS: UPEC and commensal E. coli strains 
      isolated from UTI and feces of healthy humans were compared for the presence of 
      VFs and phylogenetic groups. Association between virulence genes was investigated 
      and cluster analysis was employed. RESULTS: According to the results, among a 30 
      virulence markers tested, the pathogenicity-associated island (PAI), papAH, 
      papEF, fimH, fyuA, and traT genes prevalence were statistically significant in 
      UPEC isolates. A strong association was found between the B2 and D phylogenetic 
      groups and clinical isolates of UPEC; while, commensal isolates were mostly 
      associated with phylogenetic group A. The aggregated VFs scores were more than 
      twice higher in the UPEC isolates in comparison with the commensal isolates. 
      Interestingly, the B2 group in both UPEC and commensal isolates had the highest 
      VF scores. A strong positive association was found between several virulence 
      genes. The clustering results demonstrated that UPEC or commensal E. coli 
      isolates were highly heterogeneous due to different composition of their 
      virulence gene pool and pathogenicity islands. CONCLUSION: Genetic structure and 
      VFs of UPEC strains vary from region to region; therefore, to control the UTI, 
      the epidemiological aspects and characterization of the UPEC isolates need to be 
      investigated in different regions. Since UPEC isolates are generally originate 
      from the commensal strains, it may be feasible to reduce the UTI burden by 
      interfering the intestinal colonization, particularly in the highly pathogenic 
      clonal lineages such as B2.
FAU - Rezatofighi, Seyedeh Elham
AU  - Rezatofighi SE
AUID- ORCID: 0000-0003-1373-7346
AD  - Department of biology, Faculty of Science, Shahid Chamran University of Ahvaz, 
      Ahvaz, 6135743135, Iran. e.tofighi@yahoo.com.
FAU - Mirzarazi, Mahsa
AU  - Mirzarazi M
AD  - Department of biology, Faculty of Science, Shahid Chamran University of Ahvaz, 
      Ahvaz, 6135743135, Iran.
FAU - Salehi, Mansour
AU  - Salehi M
AD  - Department of Genetics and Molecular Biology, Medical School, Isfahan University 
      of Medical Sciences, Isfahan, Iran.
LA  - eng
PT  - Journal Article
DEP - 20210417
PL  - England
TA  - BMC Infect Dis
JT  - BMC infectious diseases
JID - 100968551
RN  - 0 (Escherichia coli Proteins)
RN  - 0 (Virulence Factors)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Case-Control Studies
MH  - Child
MH  - Child, Preschool
MH  - Escherichia coli Infections/epidemiology/*microbiology
MH  - Escherichia coli Proteins/genetics
MH  - Extraintestinal Pathogenic Escherichia coli/genetics/isolation & 
      purification/pathogenicity
MH  - Female
MH  - Genomic Islands/genetics
MH  - Humans
MH  - Infant
MH  - Infant, Newborn
MH  - Iran/epidemiology
MH  - Male
MH  - Middle Aged
MH  - Phylogeny
MH  - Prevalence
MH  - Urinary Tract Infections/epidemiology/*microbiology
MH  - *Uropathogenic Escherichia coli/classification/genetics/isolation & 
      purification/pathogenicity
MH  - Virulence/*genetics
MH  - Virulence Factors/*genetics
MH  - Young Adult
PMC - PMC8052790
OTO - NOTNLM
OT  - Extraintestinal Pathogenic E. coli
OT  - Phylogenetic group
OT  - Urinary tract infection
OT  - Uropathogenic Escherichia coli
OT  - Virulence factor
COIS- The authors declare that they have no competing interests.
EDAT- 2021/04/19 06:00
MHDA- 2021/05/01 06:00
PMCR- 2021/04/17
CRDT- 2021/04/18 20:20
PHST- 2020/06/19 00:00 [received]
PHST- 2021/04/05 00:00 [accepted]
PHST- 2021/04/18 20:20 [entrez]
PHST- 2021/04/19 06:00 [pubmed]
PHST- 2021/05/01 06:00 [medline]
PHST- 2021/04/17 00:00 [pmc-release]
AID - 10.1186/s12879-021-06036-4 [pii]
AID - 6036 [pii]
AID - 10.1186/s12879-021-06036-4 [doi]
PST - epublish
SO  - BMC Infect Dis. 2021 Apr 17;21(1):361. doi: 10.1186/s12879-021-06036-4.