PMID- 33865980
OWN - NLM
STAT- MEDLINE
DCOM- 20211124
LR  - 20211124
IS  - 1096-7184 (Electronic)
IS  - 1096-7176 (Linking)
VI  - 66
DP  - 2021 Jul
TI  - Towards bio-upcycling of polyethylene terephthalate.
PG  - 167-178
LID - S1096-7176(21)00047-1 [pii]
LID - 10.1016/j.ymben.2021.03.011 [doi]
AB  - Over 359 million tons of plastics were produced worldwide in 2018, with 
      significant growth expected in the near future, resulting in the global challenge 
      of end-of-life management. The recent identification of enzymes that degrade 
      plastics previously considered non-biodegradable opens up opportunities to steer 
      the plastic recycling industry into the realm of biotechnology. Here, the 
      sequential conversion of post-consumer polyethylene terephthalate (PET) into two 
      types of bioplastics is presented: a medium chain-length polyhydroxyalkanoate 
      (PHA) and a novel bio-based poly(amide urethane) (bio-PU). PET films are 
      hydrolyzed by a thermostable polyester hydrolase yielding highly pure 
      terephthalate and ethylene glycol. The obtained hydrolysate is used directly as a 
      feedstock for a terephthalate-degrading Pseudomonas umsongensis GO16, also 
      evolved to efficiently metabolize ethylene glycol, to produce PHA. The strain is 
      further modified to secrete hydroxyalkanoyloxy-alkanoates (HAAs), which are used 
      as monomers for the chemo-catalytic synthesis of bio-PU. In short, a novel 
      value-chain for PET upcycling is shown that circumvents the costly purification 
      of PET monomers, adding technological flexibility to the global challenge of 
      end-of-life management of plastics.
CI  - Copyright (c) 2021 The Authors. Published by Elsevier Inc. All rights reserved.
FAU - Tiso, Till
AU  - Tiso T
AD  - iAMB - Institute of Applied Microbiology. ABBt - Aachen Biology and 
      Biotechnology, RWTH Aachen University, Worringerweg 1, D-52074, Aachen, Germany.
FAU - Narancic, Tanja
AU  - Narancic T
AD  - BiOrbic - SFI Bioeconomy Research Centre, University College Dublin, Belfield, 
      Dublin 4, Ireland; School of Biomolecular and Biomedical Science and UCD Earth 
      Institute, University College Dublin, Belfield, Dublin 4, Ireland.
FAU - Wei, Ren
AU  - Wei R
AD  - Department of Microbiology and Bioprocess Technology, Institute of Biochemistry, 
      Leipzig University, Johannisallee 23, D-04103, Leipzig, Germany.
FAU - Pollet, Eric
AU  - Pollet E
AD  - BioTeam/ICPEES-ECPM, UMR CNRS 7515, Strasbourg University, 25 rue Becquerel, 
      F-67087, Strasbourg Cedex 2, France.
FAU - Beagan, Niall
AU  - Beagan N
AD  - BiOrbic - SFI Bioeconomy Research Centre, University College Dublin, Belfield, 
      Dublin 4, Ireland.
FAU - Schroder, Katja
AU  - Schroder K
AD  - iAMB - Institute of Applied Microbiology. ABBt - Aachen Biology and 
      Biotechnology, RWTH Aachen University, Worringerweg 1, D-52074, Aachen, Germany.
FAU - Honak, Annett
AU  - Honak A
AD  - Department of Microbiology and Bioprocess Technology, Institute of Biochemistry, 
      Leipzig University, Johannisallee 23, D-04103, Leipzig, Germany.
FAU - Jiang, Mengying
AU  - Jiang M
AD  - BioTeam/ICPEES-ECPM, UMR CNRS 7515, Strasbourg University, 25 rue Becquerel, 
      F-67087, Strasbourg Cedex 2, France; SOPREMA, 14 rue de Saint-Nazaire, F-67025 
      Strasbourg Cedex, France.
FAU - Kenny, Shane T
AU  - Kenny ST
AD  - Bioplastech Ltd., NovaUCD, Belfield Innovation Park, University College Dublin, 
      Belfield, Dublin 4, Ireland.
FAU - Wierckx, Nick
AU  - Wierckx N
AD  - iAMB - Institute of Applied Microbiology. ABBt - Aachen Biology and 
      Biotechnology, RWTH Aachen University, Worringerweg 1, D-52074, Aachen, Germany; 
      Institute of Bio- and Geosciences IBG-1: Biotechnology, Forschungszentrum Julich, 
      52425, Julich, Germany.
FAU - Perrin, Remi
AU  - Perrin R
AD  - SOPREMA, 14 rue de Saint-Nazaire, F-67025 Strasbourg Cedex, France.
FAU - Averous, Luc
AU  - Averous L
AD  - BioTeam/ICPEES-ECPM, UMR CNRS 7515, Strasbourg University, 25 rue Becquerel, 
      F-67087, Strasbourg Cedex 2, France.
FAU - Zimmermann, Wolfgang
AU  - Zimmermann W
AD  - Department of Microbiology and Bioprocess Technology, Institute of Biochemistry, 
      Leipzig University, Johannisallee 23, D-04103, Leipzig, Germany.
FAU - O'Connor, Kevin
AU  - O'Connor K
AD  - BiOrbic - SFI Bioeconomy Research Centre, University College Dublin, Belfield, 
      Dublin 4, Ireland; School of Biomolecular and Biomedical Science and UCD Earth 
      Institute, University College Dublin, Belfield, Dublin 4, Ireland. Electronic 
      address: kevin.oconnor@ucd.ie.
FAU - Blank, Lars M
AU  - Blank LM
AD  - iAMB - Institute of Applied Microbiology. ABBt - Aachen Biology and 
      Biotechnology, RWTH Aachen University, Worringerweg 1, D-52074, Aachen, Germany. 
      Electronic address: lars.blank@rwth-aachen.de.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210416
PL  - Belgium
TA  - Metab Eng
JT  - Metabolic engineering
JID - 9815657
RN  - 0 (Plastics)
RN  - 0 (Polyethylene Terephthalates)
RN  - EC 3.- (Hydrolases)
RN  - Pseudomonas umsongensis
SB  - IM
MH  - Hydrolases
MH  - Plastics
MH  - *Polyethylene Terephthalates
MH  - *Pseudomonas
OTO - NOTNLM
OT  - Biopolymers
OT  - Metabolic engineering
OT  - Polyethylene terephthalate (PET) degradation
OT  - Pseudomonas putida
OT  - Synthetic biology
EDAT- 2021/04/19 06:00
MHDA- 2021/11/25 06:00
CRDT- 2021/04/18 20:40
PHST- 2020/09/14 00:00 [received]
PHST- 2021/02/24 00:00 [revised]
PHST- 2021/03/13 00:00 [accepted]
PHST- 2021/04/19 06:00 [pubmed]
PHST- 2021/11/25 06:00 [medline]
PHST- 2021/04/18 20:40 [entrez]
AID - S1096-7176(21)00047-1 [pii]
AID - 10.1016/j.ymben.2021.03.011 [doi]
PST - ppublish
SO  - Metab Eng. 2021 Jul;66:167-178. doi: 10.1016/j.ymben.2021.03.011. Epub 2021 Apr 
      16.