PMID- 33867395
OWN - NLM
STAT- MEDLINE
DCOM- 20220120
LR  - 20220120
IS  - 1348-4540 (Electronic)
IS  - 0918-8959 (Linking)
VI  - 68
IP  - 8
DP  - 2021 Aug 28
TI  - Reduction of arcuate kappa-opioid receptor-expressing cells increased luteinizing 
      hormone pulse frequency in female rats.
PG  - 933-941
LID - 10.1507/endocrj.EJ20-0832 [doi]
AB  - The brain mechanism responsible for the pulsatile secretion of 
      gonadotropin-releasing hormone (GnRH) is important for maintaining reproductive 
      function in mammals. Accumulating evidence suggests that kisspeptin/neurokinin 
      B/dynorphin A (KNDy) neurons in the hypothalamic arcuate nucleus (ARC) play a 
      critical role in the regulation of pulsatile GnRH and subsequent gonadotropin 
      secretion. Dynorphin A (Dyn) and its receptor, kappa-opioid receptor (KOR, 
      encoded by Oprk1), have been shown to be involved in the suppression of pulsatile 
      GnRH/luteinizing hormone (LH) release. On the other hand, it is still unclear 
      whether the inhibitory Dyn signaling affects KNDy neurons or KOR-expressing 
      non-KNDy cells in the ARC or other brain regions. We therefore aimed to clarify 
      the role of ARC-specific Dyn-KOR signaling in the regulation of pulsatile GnRH/LH 
      release by the ARC specific cell deletion of KOR-expressing cells using 
      Dyn-conjugated-saporin (Dyn-SAP). Estrogen-primed ovariectomized female rats were 
      administered Dyn-SAP to the ARC. In situ hybridization of Oprk1 showed that ARC 
      Dyn-SAP administration significantly decreased the number of Oprk1-expressing 
      cells in the ARC, but not in the ventromedial hypothalamic nucleus and 
      paraventricular nucleus. The frequency of LH pulses significantly increased in 
      animals bearing the ARC Dyn-SAP administration. The number of Kiss1-expressing 
      cells in the ARC was not affected by ARC Dyn-SAP treatment. Dyn-KOR signaling 
      within the ARC seems to mediate the suppression of the frequency of pulsatile 
      GnRH/LH release, and ARC non-KNDy KOR neurons may be involved in the mechanism 
      modulating GnRH/LH pulse generation.
FAU - Dai, Mingdao
AU  - Dai M
AD  - Laboratory of Theriogenology, Department of Veterinary Medical Sciences, The 
      University of Tokyo, Tokyo 113-8657, Japan.
FAU - Nakamura, Sho
AU  - Nakamura S
AD  - Laboratory of Theriogenology, Department of Veterinary Medical Sciences, The 
      University of Tokyo, Tokyo 113-8657, Japan.
FAU - Takahashi, Chudai
AU  - Takahashi C
AD  - Laboratory of Theriogenology, Department of Veterinary Medical Sciences, The 
      University of Tokyo, Tokyo 113-8657, Japan.
FAU - Sato, Marimo
AU  - Sato M
AD  - Laboratory of Theriogenology, Department of Veterinary Medical Sciences, The 
      University of Tokyo, Tokyo 113-8657, Japan.
FAU - Munetomo, Arisa
AU  - Munetomo A
AD  - Laboratory of Theriogenology, Department of Veterinary Medical Sciences, The 
      University of Tokyo, Tokyo 113-8657, Japan.
FAU - Magata, Fumie
AU  - Magata F
AD  - Laboratory of Theriogenology, Department of Veterinary Medical Sciences, The 
      University of Tokyo, Tokyo 113-8657, Japan.
FAU - Uenoyama, Yoshihisa
AU  - Uenoyama Y
AD  - Laboratory of Animal Reproduction, Graduate School of Bioagricultural Sciences, 
      Nagoya University, Nagoya 464-8601, Japan.
FAU - Tsukamura, Hiroko
AU  - Tsukamura H
AD  - Laboratory of Animal Reproduction, Graduate School of Bioagricultural Sciences, 
      Nagoya University, Nagoya 464-8601, Japan.
FAU - Matsuda, Fuko
AU  - Matsuda F
AD  - Laboratory of Theriogenology, Department of Veterinary Medical Sciences, The 
      University of Tokyo, Tokyo 113-8657, Japan.
LA  - eng
PT  - Journal Article
DEP - 20210417
PL  - Japan
TA  - Endocr J
JT  - Endocrine journal
JID - 9313485
RN  - 0 (Receptors, Opioid, kappa)
RN  - 0 (Saponins)
RN  - 74913-18-1 (Dynorphins)
RN  - 9002-67-9 (Luteinizing Hormone)
SB  - IM
MH  - Animals
MH  - Arcuate Nucleus of Hypothalamus/drug effects/*metabolism
MH  - Dynorphins/administration & dosage
MH  - Female
MH  - Luteinizing Hormone/*blood
MH  - Neurons/drug effects/*metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Receptors, Opioid, kappa/*metabolism
MH  - Saponins/administration & dosage
OTO - NOTNLM
OT  - Dynorphin
OT  - Kappa-opioid receptor
OT  - Kisspeptin/neurokinin B/dynorphin A neuron
OT  - Luteinizing hormone pulse
OT  - Saporin
EDAT- 2021/04/20 06:00
MHDA- 2022/01/21 06:00
CRDT- 2021/04/19 06:10
PHST- 2021/04/20 06:00 [pubmed]
PHST- 2022/01/21 06:00 [medline]
PHST- 2021/04/19 06:10 [entrez]
AID - 10.1507/endocrj.EJ20-0832 [doi]
PST - ppublish
SO  - Endocr J. 2021 Aug 28;68(8):933-941. doi: 10.1507/endocrj.EJ20-0832. Epub 2021 
      Apr 17.