PMID- 33872132
OWN - NLM
STAT- MEDLINE
DCOM- 20210920
LR  - 20210920
IS  - 1944-0057 (Electronic)
IS  - 1944-0057 (Linking)
VI  - 38
IP  - 6
DP  - 2021 Jun
TI  - Determination of semicarbazide residue in human urine samples using liquid 
      chromatography-tandem mass spectrometry.
PG  - 922-930
LID - 10.1080/19440049.2021.1898678 [doi]
AB  - An ultra-performance liquid chromatography coupled with electrospray ionisation 
      tandem mass spectrometry (UPLC-ESI-MS/MS) with pre-column derivatisation was 
      developed and validated for the determination of semicarbazide in human urine. 
      Urine samples were derivatised with 2-nitrobenzaldehyde and subsequently 
      extracted with acetonitrile. Extracts were concentrated and then analysed by 
      UPLC-MS/MS. The time per run was 7 min. Good results were observed for the 
      linearity of matrix-matched calibration curves (R(2) > 0.99) in the concentration 
      range of 1-100 microg/L. The absolute recovery ranged from 98.7% to 108.6%, with the 
      relative standard deviations (RSDs) of 2.2%-3.6%. The limit of detection and 
      quantification for the semicarbazide was 0.5 microg/L and 1 microg/L, respectively. The 
      method showed good extraction efficiency, high sensitivity, and good 
      reproducibility. It was suitable for the detection of semicarbazide in human 
      urine. Residues of semicarbazide were between 1.0 and 41.5 mug/L in children's 
      24-h urine. This work is the first report on the quantitative analysis of SEM in 
      24-h human urine samples.
FAU - Yang, Huamei
AU  - Yang H
AD  - Taizhou Center for Disease Prevention and Control, Taizhou, China.
FAU - Jiang, Xiaoli
AU  - Jiang X
AD  - Taizhou Center for Disease Prevention and Control, Taizhou, China.
FAU - Wang, Yanli
AU  - Wang Y
AD  - Nanjing Center for Disease Prevention and Control, Nanjing, China.
FAU - Li, Chen
AU  - Li C
AD  - Taizhou Center for Disease Prevention and Control, Taizhou, China.
FAU - Hang, Li
AU  - Hang L
AD  - Taizhou Center for Disease Prevention and Control, Taizhou, China.
FAU - Huang, Weihong
AU  - Huang W
AD  - Taizhou Center for Disease Prevention and Control, Taizhou, China.
LA  - eng
PT  - Journal Article
DEP - 20210419
PL  - England
TA  - Food Addit Contam Part A Chem Anal Control Expo Risk Assess
JT  - Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & 
      risk assessment
JID - 101485040
RN  - 0 (Acetonitriles)
RN  - 0 (Benzaldehydes)
RN  - 0 (Semicarbazides)
RN  - 48B18Q9B8E (2-nitrobenzaldehyde)
RN  - Z072SB282N (acetonitrile)
SB  - IM
MH  - Acetonitriles/chemistry
MH  - Benzaldehydes/chemistry
MH  - Biosensing Techniques
MH  - Child
MH  - Child, Preschool
MH  - China
MH  - Chromatography, High Pressure Liquid
MH  - Female
MH  - Humans
MH  - Limit of Detection
MH  - Male
MH  - Reproducibility of Results
MH  - Semicarbazides/*isolation & purification/*urine
MH  - Sensitivity and Specificity
MH  - Tandem Mass Spectrometry
OTO - NOTNLM
OT  - 2-nitrobenzaldehyde
OT  - LC-MS/MS
OT  - Semicarbazide
OT  - derivatisation
OT  - urine samples
EDAT- 2021/04/20 06:00
MHDA- 2021/09/21 06:00
CRDT- 2021/04/19 17:17
PHST- 2021/04/20 06:00 [pubmed]
PHST- 2021/09/21 06:00 [medline]
PHST- 2021/04/19 17:17 [entrez]
AID - 10.1080/19440049.2021.1898678 [doi]
PST - ppublish
SO  - Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2021 
      Jun;38(6):922-930. doi: 10.1080/19440049.2021.1898678. Epub 2021 Apr 19.