PMID- 33877529 OWN - NLM STAT- MEDLINE DCOM- 20210609 LR - 20210609 IS - 1573-4978 (Electronic) IS - 0301-4851 (Linking) VI - 48 IP - 4 DP - 2021 Apr TI - Sexually dimorphic expression and regulatory sequence of dnali1 in the olive flounder Paralichthys olivaceus. PG - 3529-3540 LID - 10.1007/s11033-021-06342-9 [doi] AB - Dynein axonemal light intermediate chain 1 (dnali1) is an important part of axonemal dyneins and plays an important role in the growth and development of animals. However, there is little information about dnali1 in fish. Herein, we cloned dnali1 gene from the genome of olive flounder (Paralichthys olivaceus), a commercially important maricultured fish in China, Japan, and Korea, and analyzed its expression patterns in different gender fish. The flounder dnali1 DNA sequence contained a 771 bp open reading frame (ORF), two different sizes of 5' untranslated region (5'UTR), and a 1499 bp 3' untranslated region (3'UTR). Two duplicated 922 nt fragments were found in dnali1 mRNA. The first fragment contained the downstream coding region and the front portion of 3'UTR, and the second fragment was entirely located in 3'UTR. Multiple alignments indicated that the flounder Dnali1 protein contained the putative conserved coiled-coil domain. Its expression showed sexually dimorphic with predominant expression in the flounder testis, and lower expression in other tissues. The gene with the longer 5'UTR was specifically expressed in the testis. The highest expression level in the testis was detected at stages IV and V. Transient expression analysis showed that the 922 bp repeated sequence 3'UTR of dnali1 down-regulated the expression of GFP at the early stage in zebrafish. The flounder dnali1 might play an important role in the testis, especially in the period of spermatogenesis, and the 5'UTR and the repetitive sequences in 3'UTR might contain some regulatory elements for the cilia. FAU - Wang, Ling AU - Wang L AD - CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao, 266071, P.R. China. AD - Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, 266071, P.R. China. AD - University of Chinese Academy of Sciences, Beijing, 10049, P.R. China. FAU - Tan, Xungang AU - Tan X AD - CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao, 266071, P.R. China. tanx@qdio.ac.cn. AD - Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, 266071, P.R. China. tanx@qdio.ac.cn. FAU - Zou, Congcong AU - Zou C AD - CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao, 266071, P.R. China. AD - Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, 266071, P.R. China. AD - University of Chinese Academy of Sciences, Beijing, 10049, P.R. China. FAU - Wang, Lijuan AU - Wang L AD - CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao, 266071, P.R. China. AD - Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, 266071, P.R. China. FAU - Wu, Zhihao AU - Wu Z AD - CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao, 266071, P.R. China. AD - Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, 266071, P.R. China. FAU - Zou, Yuxia AU - Zou Y AD - CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao, 266071, P.R. China. AD - Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, 266071, P.R. China. FAU - Song, Zongcheng AU - Song Z AD - Shenghang Aquatic Science and Technology Co. Ltd, Weihai, China. FAU - You, Feng AU - You F AD - CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao, 266071, P.R. China. youfeng@qdio.ac.cn. AD - Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, 266071, P.R. China. youfeng@qdio.ac.cn. LA - eng GR - 31772834/National Sciences of Foundation of China/ GR - 31672636/National Sciences of Foundation of China/ GR - 2018YFD0900202/the National Key R&D Program of China/ GR - 2018YFD0901202/Key Technology Research and Development Program of Shandong (CN)/ GR - 31702337/Nation Sciences of Foundation of China/ PT - Journal Article DEP - 20210420 PL - Netherlands TA - Mol Biol Rep JT - Molecular biology reports JID - 0403234 RN - 0 (3' Untranslated Regions) RN - 0 (Fish Proteins) RN - EC 3.6.4.2 (Dyneins) SB - IM MH - 3' Untranslated Regions MH - Animals MH - Conserved Sequence MH - Dyneins/chemistry/*genetics/metabolism MH - Female MH - Fish Proteins/chemistry/*genetics/metabolism MH - Flounder/genetics/*metabolism MH - Male MH - Open Reading Frames MH - Protein Domains MH - Sex Characteristics MH - Testis/*metabolism OTO - NOTNLM OT - 3'UTR OT - 5'UTR OT - Dnali1 OT - Gonad OT - Repeated fragment OT - Sexually dimorphic expression EDAT- 2021/04/21 06:00 MHDA- 2021/06/10 06:00 CRDT- 2021/04/20 12:26 PHST- 2021/01/25 00:00 [received] PHST- 2021/04/07 00:00 [accepted] PHST- 2021/04/21 06:00 [pubmed] PHST- 2021/06/10 06:00 [medline] PHST- 2021/04/20 12:26 [entrez] AID - 10.1007/s11033-021-06342-9 [pii] AID - 10.1007/s11033-021-06342-9 [doi] PST - ppublish SO - Mol Biol Rep. 2021 Apr;48(4):3529-3540. doi: 10.1007/s11033-021-06342-9. Epub 2021 Apr 20.