PMID- 33880596
OWN - NLM
STAT- MEDLINE
DCOM- 20211018
LR  - 20211018
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Print)
IS  - 1791-2997 (Linking)
VI  - 23
IP  - 6
DP  - 2021 Jun
TI  - Knockdown of lncRNA DLEU1 inhibits the tumorigenesis of oral squamous cell 
      carcinoma via regulation of miR‑149‑5p/CDK6 axis.
LID - 447 [pii]
LID - 10.3892/mmr.2021.12086 [doi]
AB  - Oral squamous cell carcinoma (OSCC) is a frequent malignant tumor worldwide. Long 
      non‑coding RNAs (lncRNAs) are known to play key roles in different types of 
      cancer, including OSCC. It was previously reported that lncRNA deleted in 
      lymphocytic leukemia 1 (DLEU1) is notably upregulated in OSCC; however, the role 
      of DLEU1 in OSCC remains unclear. Gene and protein expression levels in OSCC 
      cells were detected by reverse transcription‑quantitative PCR and western 
      blotting, respectively, in the present study. A Transwell assay was performed to 
      measure cell migration and invasion. Flow cytometry was used to detect cell 
      apoptosis, and the dual‑luciferase reporter assay was applied to confirm the 
      interaction between DLEU1, microRNA (miR)‑149‑5p and CDK6 in OSCC cells. DLEU1 
      expression was negatively associated with the survival rate of patients with 
      OSCC. In addition, silencing of DLEU1 notably inhibited the proliferation of OSCC 
      cells by inducing apoptosis. Meanwhile, DLEU1 directly bound to miR‑149‑5p, and 
      CDK6 was found to be the direct target of miR‑149‑5p. Furthermore, DLEU1 
      knockdown‑induced inhibition of OSCC cell proliferation was significantly 
      reversed by the miR‑149‑5p antagomir. Knockdown of lncRNA DLEU1 reversed the 
      proliferation of OSCC cells via regulation of the miR‑149‑5p/CDK6 axis. Thus, 
      DLEU1 may serve as a novel target for treating OSCC.
FAU - Lv, Tianzhu
AU  - Lv T
AD  - Department of Emergency General, The Affiliated Stomatological Hospital of 
      Guizhou Medical University, Guiyang, Guizhou 550025, P.R. China.
FAU - Liu, Hongjing
AU  - Liu H
AD  - Department of Emergency General, The Affiliated Stomatological Hospital of 
      Guizhou Medical University, Guiyang, Guizhou 550025, P.R. China.
FAU - Wu, Yadong
AU  - Wu Y
AD  - Department of Oral and Maxillofacial Surgery, The Affiliated Stomatological 
      Hospital of Guizhou Medical University, Guiyang, Guizhou 550025, P.R. China.
FAU - Huang, Wentao
AU  - Huang W
AD  - Department of Basic Stomatology, School of Savaid Stomatology, Hangzhou Medical 
      College, Hangzhou, Zhejiang 310053, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20210421
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (DLEU1 lncRNA, human)
RN  - 0 (MIRN149 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Long Noncoding)
RN  - EC 2.7.11.22 (CDK6 protein, human)
RN  - EC 2.7.11.22 (Cyclin-Dependent Kinase 6)
SB  - IM
MH  - Apoptosis
MH  - Carcinogenesis/*drug effects/*genetics
MH  - Carcinoma, Squamous Cell/*drug therapy/pathology
MH  - Cell Line, Tumor
MH  - Cell Movement
MH  - Cell Transformation, Neoplastic/genetics
MH  - China
MH  - Cyclin-Dependent Kinase 6/genetics/*metabolism
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - Gene Knockdown Techniques
MH  - Humans
MH  - Male
MH  - MicroRNAs/genetics/*metabolism
MH  - Middle Aged
MH  - Mouth Neoplasms/*drug therapy/pathology
MH  - RNA, Long Noncoding/*genetics/*pharmacology
PMC - PMC8060799
OTO - NOTNLM
OT  - oral squamous cell carcinoma
OT  - deleted in lymphocytic leukemia 1
OT  - microRNA‑149‑5p
OT  - cyclin dependent kinase 6
COIS- The authors declare that they have no competing interests.
EDAT- 2021/04/22 06:00
MHDA- 2021/10/21 06:00
PMCR- 2021/04/13
CRDT- 2021/04/21 06:51
PHST- 2020/09/23 00:00 [received]
PHST- 2021/02/03 00:00 [accepted]
PHST- 2021/04/21 06:51 [entrez]
PHST- 2021/04/22 06:00 [pubmed]
PHST- 2021/10/21 06:00 [medline]
PHST- 2021/04/13 00:00 [pmc-release]
AID - 447 [pii]
AID - MMR-0-0-12086 [pii]
AID - 10.3892/mmr.2021.12086 [doi]
PST - ppublish
SO  - Mol Med Rep. 2021 Jun;23(6):447. doi: 10.3892/mmr.2021.12086. Epub 2021 Apr 21.