PMID- 33886273
OWN - NLM
STAT- MEDLINE
DCOM- 20210609
LR  - 20230704
IS  - 1936-086X (Electronic)
IS  - 1936-0851 (Print)
IS  - 1936-0851 (Linking)
VI  - 15
IP  - 5
DP  - 2021 May 25
TI  - Nanoparticles Interfere with Chemotaxis: An Example of Nanoparticles as Molecular 
      "Knockouts" at the Cellular Level.
PG  - 8813-8825
LID - 10.1021/acsnano.1c01262 [doi]
AB  - Engineered colloidal nanoparticles show great promise in biomedical applications. 
      While much of the work of assessing nanoparticle impact on living systems has 
      been focused on the direct interactions of nanoparticles with cells/organisms, 
      indirect effects via the extracellular matrix have been observed and may provide 
      deeper insight into nanoparticle fate and effects in living systems. In 
      particular, the large surface area of colloidal nanoparticles may sequester 
      molecules from the biological milieu, make these molecules less bioavailable, and 
      therefore function indirectly as "molecular knockouts" to exert effects at the 
      cellular level and beyond. In this paper, the hypothesis that molecules that 
      control cellular behavior (in this case, chemoattract molecules that promote 
      migration of a human monocytic cell line, THP-1) will be less bioavailable in the 
      presence of appropriately functionalized nanoparticles, and therefore the 
      cellular behavior will be altered, was investigated. Three-dimensional chemotaxis 
      assays for the characterization and comparison of THP-1 cell migration upon 
      exposure to a gradient of monocyte chemoattractant protein-1 (MCP-1), with and 
      without gold nanoparticles with four different surface chemistries, were 
      performed. By time-lapse microscopy, characteristic parameters for chemotaxis, 
      along with velocity and directionality of the cells, were quantified. Anionic 
      poly(sodium 4-styrenesulfonate)-coated gold nanoparticles were found to 
      significantly reduce THP-1 chemotaxis. Enzyme-linked immunosorbent assay results 
      show adsorption of MCP-1 on the poly(sodium 4-styrenesulfonate)-coated gold 
      nanoparticle surface, supporting the hypothesis that adsorption of 
      chemoattractants to nanoparticle surfaces interferes with chemotaxis. Free 
      anionic sulfonated polyelectrolytes also interfered with cell migrational 
      behavior, showing that nanoparticles can also act as carriers of 
      chemotactic-interfering molecules.
FAU - Zhang, Xi
AU  - Zhang X
AUID- ORCID: 0000-0001-5777-2368
AD  - Department of Chemistry, University of Illinois at Urbana-Champaign, 600 S. 
      Mathews Avenue, Urbana, Illinois 61801, United States.
FAU - Falagan-Lotsch, Priscila
AU  - Falagan-Lotsch P
AUID- ORCID: 0000-0002-0849-2682
AD  - Department of Chemistry, University of Illinois at Urbana-Champaign, 600 S. 
      Mathews Avenue, Urbana, Illinois 61801, United States.
AD  - Department of Biological Sciences, Auburn University, Auburn, Alabama 36849, 
      United States.
FAU - Murphy, Catherine J
AU  - Murphy CJ
AUID- ORCID: 0000-0001-7066-5575
AD  - Department of Chemistry, University of Illinois at Urbana-Champaign, 600 S. 
      Mathews Avenue, Urbana, Illinois 61801, United States.
LA  - eng
GR  - R01 GM125845/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20210422
PL  - United States
TA  - ACS Nano
JT  - ACS nano
JID - 101313589
RN  - 0 (Polyelectrolytes)
RN  - 7440-57-5 (Gold)
SB  - IM
MH  - Chemotaxis
MH  - Gold
MH  - Humans
MH  - *Metal Nanoparticles
MH  - Monocytes
MH  - *Nanoparticles
MH  - Polyelectrolytes
PMC - PMC10312996
MID - NIHMS1908131
OTO - NOTNLM
OT  - cell migration
OT  - chemotaxis
OT  - gold nanoparticles
OT  - nanoparticle intervention
OT  - three-dimensional model
COIS- The authors declare no competing financial interest.
EDAT- 2021/04/23 06:00
MHDA- 2021/06/10 06:00
PMCR- 2023/06/30
CRDT- 2021/04/22 17:13
PHST- 2021/04/23 06:00 [pubmed]
PHST- 2021/06/10 06:00 [medline]
PHST- 2021/04/22 17:13 [entrez]
PHST- 2023/06/30 00:00 [pmc-release]
AID - 10.1021/acsnano.1c01262 [doi]
PST - ppublish
SO  - ACS Nano. 2021 May 25;15(5):8813-8825. doi: 10.1021/acsnano.1c01262. Epub 2021 
      Apr 22.