PMID- 33886608
OWN - NLM
STAT- MEDLINE
DCOM- 20211012
LR  - 20211012
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 16
IP  - 4
DP  - 2021
TI  - Complex effects of whole body cryostimulation on hematological markers in 
      patients with obesity.
PG  - e0249812
LID - 10.1371/journal.pone.0249812 [doi]
LID - e0249812
AB  - BACKGROUND: Adaptation, including changes in blood properties, to whole-body 
      cryostimulation may depend on many factors, including body mass. AIM: This study 
      investigates whether hematological parameters change similarly in a group of 
      people with obesity and a group of men with normal body weight after 10 and 20 
      cryostimulation treatments. METHODS: In our non-randomized trial, the 
      participants were divided into two groups based on their body fat percentage: 14 
      men with a high (HBF = 29.35%) and 10 with a normal percent of body fat (NBF = 
      11.40%) and subjected to 20 whole body cryostimulation treatments (-120 degrees C for 2-3 
      minutes). Blood samples were taken before the first and after the 10th and 20th 
      cryostimulation. The following parameters were determined: red blood cells (RBC), 
      hemoglobin concentration (HGB), hematocrit (HCT), mean corpuscular volume (MCV), 
      mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration 
      (MCHC), platelets (PLT), red blood cell distribution width (RDW-SD), mean 
      platelet volume (MPV), white blood cells (WBC), neutrophils (NEUT), lymphocytes 
      (LYMPH), monocytes (MONO), eosinophils (EO) and basophiles (BASO). RESULTS: 
      Statistically significant differences were found in red blood cells parameters 
      such as RBC, HCT, MCV and MCHC. Time influence was noted for HCT, MCV and MCHC. 
      Two-way ANOVA showed a significant correlation (for time and group) for 2 
      paramateres: RBC and MCV. For platelet parameters statistically significant 
      differences were found for PLT (group influence) and MPV (time and group 
      interaction). In white blood cells parameters statistically significant 
      differences in levels of LYMPH were noted. Higher levels were observed for HBF 
      group. CONCLUSIONS: All observed changes were within the reference range, but 
      hematological markers changed unevenly in people who are obese and non-obese. 
      Therefore, it appears that an amount of fat tissue could be a factor causing the 
      differences in adaptation to low temperature. It is suggested that 20 whole body 
      cryostimulation sessions restore the state of homeostasis disturbed after 10 
      sessions. TRIAL REGISTRATION: ACTRN 12619000524190.
FAU - Wyrostek, Joanna
AU  - Wyrostek J
AUID- ORCID: 0000-0002-9293-9941
AD  - Faculty of Physiotherapy, University of Physical Education in Krakow, Krakow, 
      Poland.
FAU - Piotrowska, Anna
AU  - Piotrowska A
AD  - Institute for Basics Sciences, Faculty of Physiotherapy, University of Physical 
      Education in Krakow, Krakow, Poland.
FAU - Czerwinska-Ledwig, Olga
AU  - Czerwinska-Ledwig O
AD  - Institute for Basics Sciences, Faculty of Physiotherapy, University of Physical 
      Education in Krakow, Krakow, Poland.
FAU - Zuziak, Roxana
AU  - Zuziak R
AD  - Institute for Basics Sciences, Faculty of Physiotherapy, University of Physical 
      Education in Krakow, Krakow, Poland.
FAU - Szygula, Zbigniew
AU  - Szygula Z
AD  - Institute of Biomedical Sciences, Department of Sports Medicine and Human 
      Nutrition, University of Physical Education in Krakow, Krakow, Poland.
FAU - Cison, Tomasz
AU  - Cison T
AD  - Department of Physiotherapy, State University of Applied Science in Nowy Sacz, 
      Nowy Sacz, Poland.
FAU - Zychowska, Malgorzata
AU  - Zychowska M
AD  - Department of Sport, Faculty of Physical Education, Kazimierz Wielki University 
      in Bydgoszcz, Bydgoszcz, Poland.
FAU - Pilch, Wanda
AU  - Pilch W
AD  - Institute for Basics Sciences, Faculty of Physiotherapy, University of Physical 
      Education in Krakow, Krakow, Poland.
LA  - eng
SI  - ANZCTR/ACTRN 12619000524190
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210422
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Biomarkers)
SB  - IM
MH  - Adaptation, Physiological/*physiology
MH  - Adult
MH  - Biomarkers/blood
MH  - Cryotherapy/*methods
MH  - Erythrocyte Indices
MH  - Hematocrit
MH  - Hematologic Tests/methods
MH  - Humans
MH  - Male
MH  - Obesity/*blood/*therapy
MH  - Reference Values
MH  - Young Adult
PMC - PMC8062033
COIS- The authors have declared that no competing interests exist.
EDAT- 2021/04/23 06:00
MHDA- 2021/10/13 06:00
PMCR- 2021/04/22
CRDT- 2021/04/22 17:29
PHST- 2020/07/23 00:00 [received]
PHST- 2021/03/23 00:00 [accepted]
PHST- 2021/04/22 17:29 [entrez]
PHST- 2021/04/23 06:00 [pubmed]
PHST- 2021/10/13 06:00 [medline]
PHST- 2021/04/22 00:00 [pmc-release]
AID - PONE-D-20-18930 [pii]
AID - 10.1371/journal.pone.0249812 [doi]
PST - epublish
SO  - PLoS One. 2021 Apr 22;16(4):e0249812. doi: 10.1371/journal.pone.0249812. 
      eCollection 2021.