PMID- 33891231 OWN - NLM STAT- MEDLINE DCOM- 20211206 LR - 20211214 IS - 1573-6776 (Electronic) IS - 0141-5492 (Linking) VI - 43 IP - 7 DP - 2021 Jul TI - In vitro facilitating role of polygonatum sibiricum polysaccharide in osteogenic differentiation of bone marrow mesenchymal stem cells from patients with multiple myeloma. PG - 1311-1322 LID - 10.1007/s10529-021-03125-x [doi] AB - BACKGROUND: Bone marrow mesenchymal stem cells (BMMSCs) were proved to play a vital role in multiple myeloma (MM). Polygonatum sibiricum polysaccharide (PSP) was found to have anti-tumor pharmacological effects, yet its interaction with BMMSCs remained poorly understood. Therefore, we explore the effect of PSP on osteogenic differentiation of BMMSCs. METHODS: BMMSCs were isolated by density gradient centrifugation. CD90 and CD34 were detected by flow cytometry (FCM). Osteogenic marks were detected by quantitative real-time PCR (qRT-PCR) and Western blotting (WB). The vitality of cells treated with different concentrations of PSP was observed by Cell Counting Kit-8 (CCK-8). ALP staining kit was used to detect the activity of alkaline phosphatase (ALP). Alizarin red staining detected the formation of mineralized nodules. Osteoblast-associated genes were evaluated by qRT-PCR and WB. The phosphoinositide 3-kinase (PI3K), protein kinase B (AKT), and mammalian target of rapamycin (mTOR) signaling pathways were tested by WB. RESULTS: The BMMSCs showed good growth under an inverted microscope. FCM showed that CD34 and CD45 was low-expressed, whereas CD44, CD90 and CD105 was highly expressed. Compared with the Control group, the expressions of Runx2 and ALP in cells were significantly increased. CCK-8 showed that different concentrations of PSP had no significant effect on the viability of BMMSCs. BMMSCs treated with 25 mg/l PSP were stained the most deeply by ALP. Mineralized nodules in PSP groups dramatically increased, and hit a peak under the action of 25 mg/l PSP. PSP up-regulated p-PI3K, p-AKT, and p-mTOR, but had no significant effect on PI3K, AKT, and mTOR. CONCLUSION: PSP induced osteogenic differentiation of BMMSCs from MM patients. FAU - Zhao, Jianqiang AU - Zhao J AD - Department of Hematology, The First Affiliated Hospital of Xi'an Medical University, No. 48, Fenghao West Road, Lianhu District, Xi'an, 710077, China. FAU - Ma, Lijie AU - Ma L AUID- ORCID: 0000-0002-2015-2314 AD - Department of Hematology, The First Affiliated Hospital of Xi'an Medical University, No. 48, Fenghao West Road, Lianhu District, Xi'an, 710077, China. malijie_ljiema@163.com. FAU - Ni, Zengfeng AU - Ni Z AD - Department of Hematology, The First Affiliated Hospital of Xi'an Medical University, No. 48, Fenghao West Road, Lianhu District, Xi'an, 710077, China. FAU - Liu, Hui AU - Liu H AD - Department of Hematology, The First Affiliated Hospital of Xi'an Medical University, No. 48, Fenghao West Road, Lianhu District, Xi'an, 710077, China. LA - eng PT - Journal Article DEP - 20210423 PL - Netherlands TA - Biotechnol Lett JT - Biotechnology letters JID - 8008051 RN - 0 (Biomarkers, Tumor) RN - 0 (Core Binding Factor Alpha 1 Subunit) RN - 0 (Plant Extracts) RN - 0 (Polysaccharides) RN - 0 (RUNX2 protein, human) RN - EC 2.7.1.1 (MTOR protein, human) RN - EC 2.7.1.137 (Phosphatidylinositol 3-Kinase) RN - EC 2.7.11.1 (AKT1 protein, human) RN - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt) RN - EC 2.7.11.1 (TOR Serine-Threonine Kinases) RN - EC 3.1.3.1 (Alkaline Phosphatase) SB - IM MH - Alkaline Phosphatase/genetics MH - Biomarkers, Tumor/*genetics MH - Cell Differentiation/drug effects MH - Cells, Cultured MH - Core Binding Factor Alpha 1 Subunit/genetics MH - Gene Expression Regulation, Neoplastic/drug effects MH - Humans MH - Mesenchymal Stem Cells/chemistry/*cytology/drug effects MH - Models, Biological MH - Multiple Myeloma/drug therapy/genetics/*pathology MH - Osteogenesis/*drug effects MH - Phosphatidylinositol 3-Kinase/metabolism MH - Phosphorylation/drug effects MH - Plant Extracts/pharmacology MH - Polygonatum/*chemistry MH - Polysaccharides/*pharmacology MH - Proto-Oncogene Proteins c-akt/metabolism MH - Signal Transduction/drug effects MH - TOR Serine-Threonine Kinases/metabolism OTO - NOTNLM OT - Bone marrow mesenchymal stem cells OT - Multiple myeloma OT - Osteogenic differentiation OT - Polygonatum sibiricum polysaccharide EDAT- 2021/04/24 06:00 MHDA- 2021/12/15 06:00 CRDT- 2021/04/23 12:32 PHST- 2020/08/18 00:00 [received] PHST- 2021/03/27 00:00 [accepted] PHST- 2021/04/24 06:00 [pubmed] PHST- 2021/12/15 06:00 [medline] PHST- 2021/04/23 12:32 [entrez] AID - 10.1007/s10529-021-03125-x [pii] AID - 10.1007/s10529-021-03125-x [doi] PST - ppublish SO - Biotechnol Lett. 2021 Jul;43(7):1311-1322. doi: 10.1007/s10529-021-03125-x. Epub 2021 Apr 23.