PMID- 33893122
OWN - NLM
STAT- MEDLINE
DCOM- 20210826
LR  - 20211212
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Print)
IS  - 0099-2240 (Linking)
VI  - 87
IP  - 13
DP  - 2021 Jun 11
TI  - Simultaneous Anaerobic and Aerobic Ammonia and Methane Oxidation under Oxygen 
      Limitation Conditions.
PG  - e0004321
LID - 10.1128/AEM.00043-21 [doi]
LID - e00043-21
AB  - Methane and ammonia have to be removed from wastewater treatment effluent in 
      order to discharge it to receiving water bodies. A potential solution for this is 
      a combination of simultaneous ammonia and methane oxidation by anaerobic ammonia 
      oxidation (anammox) bacteria and nitrite/nitrate-dependent anaerobic methane 
      oxidation (N-damo) microorganisms. When applied, these microorganisms will be 
      exposed to oxygen, but little is known about the effect of a low concentration of 
      oxygen on a culture containing these microorganisms. In this study, a stable 
      coculture containing anammox and N-damo microorganisms in a laboratory scale 
      bioreactor was established under oxygen limitation. Membrane inlet mass 
      spectrometry (MIMS) was used to directly measure the in situ simultaneous 
      activity of N-damo, anammox, and aerobic ammonia-oxidizing microorganisms. In 
      addition, batch tests revealed that the bioreactor also harbored aerobic 
      methanotrophs and anaerobic methanogens. Together with fluorescence in situ 
      hybridization (FISH) analysis and metagenomics, these results indicate that the 
      combination of N-damo and anammox activity under the continuous supply of 
      limiting oxygen concentrations is feasible and can be implemented for the removal 
      of methane and ammonia from anaerobic digester effluents. IMPORTANCE Nitrogen in 
      wastewater leads to eutrophication of the receiving water bodies, and methane is 
      a potent greenhouse gas; it is therefore important that these are removed from 
      wastewater. A potential solution for the simultaneous removal of nitrogenous 
      compounds and methane is the application of a combination of 
      nitrite/nitrate-dependent methane oxidation (N-damo) and anaerobic ammonia 
      oxidation (annamox). In order to do so, it is important to investigate the effect 
      of oxygen on these two anaerobic processes. In this study, we investigate the 
      effect of a continuous oxygen supply on the activity of an anaerobic methane- and 
      ammonia-oxidizing coculture. The findings presented in this study are important 
      for the potential application of these two microbial processes in wastewater 
      treatment.
FAU - van Kessel, Maartje A H J
AU  - van Kessel MAHJ
AD  - Department of Microbiology, IWWR, Radboud University, Nijmegen, the Netherlands.
FAU - Stultiens, Karin
AU  - Stultiens K
AD  - Department of Microbiology, IWWR, Radboud University, Nijmegen, the Netherlands.
FAU - Pol, Arjan
AU  - Pol A
AD  - Department of Microbiology, IWWR, Radboud University, Nijmegen, the Netherlands.
FAU - Jetten, Mike S M
AU  - Jetten MSM
AD  - Department of Microbiology, IWWR, Radboud University, Nijmegen, the Netherlands.
AD  - Soehngen Institute of Anaerobic Microbiology, Nijmegen, the Netherlands.
FAU - Kartal, Boran
AU  - Kartal B
AD  - Microbial Physiology Group, Max Plank Institute for Marine Microbiology, Bremen, 
      Germany.
FAU - Op den Camp, Huub J M
AU  - Op den Camp HJM
AD  - Department of Microbiology, IWWR, Radboud University, Nijmegen, the Netherlands.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210611
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (Water Pollutants, Chemical)
RN  - 7664-41-7 (Ammonia)
RN  - OP0UW79H66 (Methane)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Aerobiosis
MH  - Ammonia/*metabolism
MH  - Anaerobiosis
MH  - Archaea/metabolism
MH  - Bacteria/metabolism
MH  - Bioreactors
MH  - Methane/*metabolism
MH  - Oxidation-Reduction
MH  - *Oxygen
MH  - Water Pollutants, Chemical/*metabolism
MH  - Water Purification/*methods
PMC - PMC8406961
OTO - NOTNLM
OT  - ammonia oxidation
OT  - anaerobic methane oxidation
OT  - anammox
OT  - methane oxidation
OT  - nitrification
OT  - oxygen exposure
OT  - wastewater treatment
EDAT- 2021/04/25 06:00
MHDA- 2021/08/27 06:00
PMCR- 2021/12/11
CRDT- 2021/04/24 05:39
PHST- 2021/04/25 06:00 [pubmed]
PHST- 2021/08/27 06:00 [medline]
PHST- 2021/04/24 05:39 [entrez]
PHST- 2021/12/11 00:00 [pmc-release]
AID - AEM.00043-21 [pii]
AID - 00043-21 [pii]
AID - 10.1128/AEM.00043-21 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2021 Jun 11;87(13):e0004321. doi: 10.1128/AEM.00043-21. 
      Epub 2021 Jun 11.