PMID- 33893613
OWN - NLM
STAT- MEDLINE
DCOM- 20211228
LR  - 20211228
IS  - 1699-3055 (Electronic)
IS  - 1699-048X (Linking)
VI  - 23
IP  - 10
DP  - 2021 Oct
TI  - Knockdown of long non-coding RNA RP11-297P16.3 inhibits the migration and 
      invasion of laryngeal squamous carcinoma cells.
PG  - 2057-2065
LID - 10.1007/s12094-021-02609-6 [doi]
AB  - PURPOSE: Laryngeal cancer has a poor prognosis when progressing to an advanced 
      stage with limited treatment options. Therefore, understanding the underlying 
      mechanisms is important to identify novel treatment targets. Long non-coding RNAs 
      (lncRNAs) have been shown to play oncogenic roles in cancer, including in 
      laryngeal cancer. We previously discovered that the lncRNA RP11-297P16.3 is 
      overexpressed in laryngeal squamous cell carcinoma (LSCC) based on RNA-sequencing 
      data. Therefore, the aim of the present study was to investigate the effects of 
      knockdown of RP11-297P16.3 on the migration and invasion of LSCC cells, and the 
      significance of these effects. METHODS: Six methods were employed to assess the 
      function of RP11-297P16.3 including gene silencing, RT-PCR, the 
      5-Ethynyl-20-deoxyuridine (EdU) staining assay, Scratch wound-healing assay, 
      transwell assay, and Western blot. RESULTS: The results show that the expression 
      of RP11-297P16.3 in the si-lncRNA group was significantly decreased compared with 
      those in the BC (blank control) and NC (negative control) groups. Moreover, 
      knockdown of RP11-297P16.3 significantly inhibited the migration and invasion of 
      LSCC cells but had no effect on cell proliferation. The protein expression of 
      N-cadherin and vimentin was notably decreased after RP11-297P16.3 knockdown; 
      whereas, the protein expression of cadherin was significantly increased 
      CONCLUSION: These results suggested that RP11-297P16.3 may inhibit the migration 
      and invasion of LSCC cells by regulating the epithelial-mesenchymal transition 
      process, suggesting that RP11-297P16.3 is a potential new target for treating 
      LSCC.
CI  - (c) 2021. Federacion de Sociedades Espanolas de Oncologia (FESEO).
FAU - Wu, H
AU  - Wu H
AUID- ORCID: 0000-0002-7317-7670
AD  - School of Basic Medical Science, Shanxi Medical University, Jinzhong, Shanxi, 
      030600, P.R. China.
FAU - Wang, W
AU  - Wang W
AD  - School of Basic Medical Science, Shanxi Medical University, Jinzhong, Shanxi, 
      030600, P.R. China.
FAU - Zhu, J
AU  - Zhu J
AD  - School of Basic Medical Science, Shanxi Medical University, Jinzhong, Shanxi, 
      030600, P.R. China. 85567411@qq.com.
LA  - eng
GR  - 2019L0411/The Science and Technology Innovation Program for Colleges and 
      Universities of Shanxi Education Department/
GR  - 81902513/The National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20210423
PL  - Italy
TA  - Clin Transl Oncol
JT  - Clinical & translational oncology : official publication of the Federation of 
      Spanish Oncology Societies and of the National Cancer Institute of Mexico
JID - 101247119
RN  - 0 (Cadherins)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (VIM protein, human)
RN  - 0 (Vimentin)
SB  - IM
MH  - Cadherins/metabolism
MH  - Cell Line, Tumor
MH  - Cell Movement/*genetics
MH  - Cell Proliferation/*genetics
MH  - Epithelial-Mesenchymal Transition/genetics
MH  - *Gene Silencing
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics/metabolism/pathology
MH  - Neoplasm Invasiveness/genetics
MH  - RNA, Long Noncoding/*genetics
MH  - Squamous Cell Carcinoma of Head and Neck/*genetics/metabolism/pathology
MH  - Vimentin/metabolism
OTO - NOTNLM
OT  - Cell invasion
OT  - Cell migration
OT  - Epithelial-mesenchymal transition
OT  - Laryngeal squamous cell carcinoma
OT  - Long non-coding RNA
EDAT- 2021/04/25 06:00
MHDA- 2021/12/29 06:00
CRDT- 2021/04/24 06:14
PHST- 2021/01/19 00:00 [received]
PHST- 2021/03/23 00:00 [accepted]
PHST- 2021/04/25 06:00 [pubmed]
PHST- 2021/12/29 06:00 [medline]
PHST- 2021/04/24 06:14 [entrez]
AID - 10.1007/s12094-021-02609-6 [pii]
AID - 10.1007/s12094-021-02609-6 [doi]
PST - ppublish
SO  - Clin Transl Oncol. 2021 Oct;23(10):2057-2065. doi: 10.1007/s12094-021-02609-6. 
      Epub 2021 Apr 23.