PMID- 33893621
OWN - NLM
STAT- MEDLINE
DCOM- 20220307
LR  - 20220307
IS  - 2629-3277 (Electronic)
IS  - 2629-3277 (Linking)
VI  - 17
IP  - 5
DP  - 2021 Oct
TI  - Adipose Derived Mesenchymal Stem Cells from a Hypoxic Culture Reduce Cartilage 
      Damage.
PG  - 1796-1809
LID - 10.1007/s12015-021-10169-z [doi]
AB  - The method to benifit tissue engineering of adipose-derived stem cells (ADSCs) to 
      cartilage has been an objective of intense research in treating increasing 
      cartilage-related disease. In this study, whether hypoxic expansion would enhance 
      the proliferation and in vitro chondrogenic differentiation of ADSCs was studied, 
      and then hypoxic expansion was applied to reduce cartilage damage in a rat model 
      in vivo. Hypoxic expansion increased the proliferation and decreased the 
      expression of aging-related genes, including p16, p21, and p53, of human ADSCs in 
      comparison with normoxic expansion. In addition, the gammaH2AX expression was reduced 
      in the hypoxic ADSCs. The chondrogenic markers were enhanced in the hypoxic ADSC 
      differentiated chondrogenic pellets, including SOX9 on day 7 and gene expressions 
      of COL 2 and COL 10 on day 21. To determine the in vitro chondrogenic 
      differentiation potential of ADSCs, ADSC differentiated 21-day chondrogenic 
      pellets were stained by Alcian blue staining and the immunostaining of COL 2 and 
      COL 10, the results of which confirmed the enhancement of differentiation 
      potential after the hypoxic expansion. Moreover, cartilage injury in a rat model 
      was reduced by hypoxic ADSC treatment that was determined by histological and 
      immunohistochemical staining detections. The effects of hypoxic expansion of 
      ADSCs and bone marrow-derived stem cells (BMSCs) on chondrogenic differentiation 
      potential were also compared. Smaller sizes were presented in the in vitro 
      hypoxic BMSC differentiated chondrogenic pellets, whereas the chondrogenic marker 
      expressions were significantly higher than those of the hypoxic ADSCs. However, 
      there was no significant difference between the treatments of the hypoxic ADSCs 
      and BMSCs in the cartilage injury in vivo. In conclusion, hypoxic expansion 
      increases the chondrogenic differentiation potential of ADSCs and BMSCs in vitro 
      and enhances them to reduce cartilage damage in vivo. Although the hypoxic BMSCs 
      showed compact chondrogenic pellet formation and higher potential of 
      chondrogenesis, the easy access and large resources of ADSCs still uplifted the 
      application.
CI  - (c) 2021. The Author(s), under exclusive licence to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Wang, Jung-Pan
AU  - Wang JP
AUID- ORCID: 0000-0002-8427-9963
AD  - School of Medicine, Department of Surgery, National Yang Ming Chiao Tung 
      University, Taipei, Taiwan. jpwang@vghtpe.gov.tw.
AD  - Department of Orthopaedics & Traumatology, Taipei Veterans General Hospital, 
      Taipei, Taiwan. jpwang@vghtpe.gov.tw.
FAU - Liao, Yu-Ting
AU  - Liao YT
AD  - Department of Orthopaedics & Traumatology, Taipei Veterans General Hospital, 
      Taipei, Taiwan.
FAU - Wu, Szu-Hsien
AU  - Wu SH
AD  - School of Medicine, Department of Surgery, National Yang Ming Chiao Tung 
      University, Taipei, Taiwan.
AD  - Department of Surgery, Division of Plastic and Reconstructive Surgery, Taipei 
      Veterans General Hospital, Taipei, Taiwan.
FAU - Huang, Hui-Kuang
AU  - Huang HK
AD  - School of Medicine, Department of Surgery, National Yang Ming Chiao Tung 
      University, Taipei, Taiwan.
AD  - Department of Orthopaedics & Traumatology, Taipei Veterans General Hospital, 
      Taipei, Taiwan.
AD  - Department of Orthopaedics, Ditmanson Medical Foundation Chiayi Christian 
      Hospital, Chiayi, Taiwan.
AD  - Chung Hwa University of Medical Technology, Tainan, Taiwan.
FAU - Chou, Po-Hsin
AU  - Chou PH
AD  - Department of Orthopaedics & Traumatology, Taipei Veterans General Hospital, 
      Taipei, Taiwan.
FAU - Chiang, En-Rung
AU  - Chiang ER
AD  - School of Medicine, Department of Surgery, National Yang Ming Chiao Tung 
      University, Taipei, Taiwan.
AD  - Department of Orthopaedics & Traumatology, Taipei Veterans General Hospital, 
      Taipei, Taiwan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210424
PL  - United States
TA  - Stem Cell Rev Rep
JT  - Stem cell reviews and reports
JID - 101752767
SB  - IM
MH  - *Adipocytes
MH  - Adipose Tissue
MH  - Animals
MH  - Cartilage
MH  - Cell Differentiation
MH  - Chondrogenesis/genetics
MH  - *Mesenchymal Stem Cells
MH  - Rats
OTO - NOTNLM
OT  - Adipose-derived stem cells (ADSCs)
OT  - Bone marrow-derived stem cells (BMSCs)
OT  - Chondrogenic differentiation, cartilage repair
OT  - Hypoxic expansion
EDAT- 2021/04/25 06:00
MHDA- 2022/03/08 06:00
CRDT- 2021/04/24 06:14
PHST- 2021/04/13 00:00 [accepted]
PHST- 2021/04/25 06:00 [pubmed]
PHST- 2022/03/08 06:00 [medline]
PHST- 2021/04/24 06:14 [entrez]
AID - 10.1007/s12015-021-10169-z [pii]
AID - 10.1007/s12015-021-10169-z [doi]
PST - ppublish
SO  - Stem Cell Rev Rep. 2021 Oct;17(5):1796-1809. doi: 10.1007/s12015-021-10169-z. 
      Epub 2021 Apr 24.