PMID- 33895907
OWN - NLM
STAT- MEDLINE
DCOM- 20210818
LR  - 20210818
IS  - 1572-9699 (Electronic)
IS  - 0003-6072 (Linking)
VI  - 114
IP  - 7
DP  - 2021 Jul
TI  - Sulfitobacter alexandrii sp. nov., a new microalgae growth-promoting bacterium 
      with exopolysaccharides bioflocculanting potential isolated from marine 
      phycosphere.
PG  - 1091-1106
LID - 10.1007/s10482-021-01580-0 [doi]
AB  - Marine phycosphere harbors unique cross-kingdom associations with enormous 
      ecological significance in aquatic ecosystems as well as relevance for algal 
      biotechnology industry. During our investigating the microbial composition and 
      bioactivity of marine phycosphere microbiota (PM), a novel lightly yellowish and 
      versatile bacterium designated strain AM1-D1(T) was isolated from cultivable PM 
      of marine dinoflagellate Alexandrium minutum amtk4 that produces high levels of 
      paralytic shellfish poisoning toxins (PSTs). Strain AM1-D1(T) demonstrates 
      notable bioflocculanting bioactivity with bacterial exopolysaccharides (EPS), and 
      microalgae growth-promoting (MGP) potential toward its algal host. Phylogenetic 
      analysis based on 16S rRNA gene sequences revealed that strain AM1-D1(T) was 
      affiliated to the members of genus Sulfitobacter within the family 
      Rhodobacteraceae, showing the highest sequence similarity of 97.9% with 
      Sulfitobacter noctilucae NB-68(T), and below 97.8% with other type strains. The 
      complete genome of strain AM1-D1(T) consisted of a circular 3.84-Mb chromosome 
      and five circular plasmids (185, 95, 15, 205 and 348 Kb, respectively) with the 
      G+C content of 64.6%. Low values obtained by phylogenomic calculations on the 
      average nucleotide identity (ANI, 77.2%), average amino acid identity (AAI, 
      74.7%) and digital DNA-DNA hybridization (dDDH, 18.6%) unequivocally separated 
      strain AM1-D1(T) from its closest relative. The main polar lipids were identified 
      as phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, 
      diphosphatidylglycerol, one unidentified phospholipid and one unidentified lipid. 
      The predominant fatty acids (> 10%) were C(18:1) omega7c, C(19:0) cyclo omega8c and 
      C(16:0). The respiratory quinone was Q-10. The genome of strain AM1-D1(T) was 
      predicted to encode series of gene clusters responsible for sulfur oxidation 
      (sox) and utilization of dissolved organic sulfur exometabolites from marine 
      dinoflagellates, taurine (tau) and dimethylsulfoniopropionate (DMSP) (dmd), as 
      well as supplementary vitamin B(12) (cob), photosynthesis carotenoids (crt) which 
      are pivotal components during algae-bacteria interactions. Based on the evidences 
      by the polyphasic characterizations, strain AM1-D1(T) represents a novel species 
      of the genus Sulfitobacter, for which the name Sulfitobacter alexandrii sp. nov. 
      is proposed. The type strain is AM1-D1(T) (= CCTCC 2017277T = KCTC 62491(T)).
FAU - Yang, Qiao
AU  - Yang Q
AD  - Department of Marine Chemistry, Zhejiang Ocean University, Zhoushan, China.
AD  - ABI Group of GPM Project, Zhejiang Ocean University, Zhoushan, China.
FAU - Ge, Ya-Ming
AU  - Ge YM
AD  - National Engineering Research Center for Marine Aquaculture, Zhoushan, China.
FAU - Iqbal, Nurhezreen Md
AU  - Iqbal NM
AD  - Malaysia Genome Institute, National Institute of Biotechnology Malaysia, Kajang, 
      Malaysia.
FAU - Yang, Xi
AU  - Yang X
AD  - Institute of Animal Science, Guangdong Academy of Agricultural Sciences, 
      Guangzhou, China.
FAU - Zhang, Xiao-Ling
AU  - Zhang XL
AD  - Department of Marine Chemistry, Zhejiang Ocean University, Zhoushan, China. 
      zhangxiaoling@zjou.edu.cn.
LA  - eng
GR  - 41876114/National Natural Science Foundation of China/
GR  - 2020B0202080004/Key Research and Development Program of Guangdong/
GR  - 2020A1313030131/Special Fund of Yinling of Guangdong/
GR  - LY18D060007/NSF of Zhejiang/
PT  - Journal Article
DEP - 20210424
PL  - Netherlands
TA  - Antonie Van Leeuwenhoek
JT  - Antonie van Leeuwenhoek
JID - 0372625
RN  - 0 (DNA, Bacterial)
RN  - 0 (Fatty Acids)
RN  - 0 (Phospholipids)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - Sulfitobacter noctilucae
SB  - IM
MH  - Bacterial Typing Techniques
MH  - DNA, Bacterial
MH  - Fatty Acids/analysis
MH  - *Microalgae
MH  - *Microbiota
MH  - Phospholipids
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - *Rhodobacteraceae/genetics
MH  - Sequence Analysis, DNA
OTO - NOTNLM
OT  - Alexandrium minutum
OT  - Algae-bacteria interactions
OT  - Microalgae growth-promoting bacterium
OT  - Paralytic shellfish poisoning toxins
OT  - Phycosphere microbiota
OT  - Sulfitobacter alexandrii
EDAT- 2021/04/26 06:00
MHDA- 2021/08/19 06:00
CRDT- 2021/04/25 20:55
PHST- 2021/02/01 00:00 [received]
PHST- 2021/04/13 00:00 [accepted]
PHST- 2021/04/26 06:00 [pubmed]
PHST- 2021/08/19 06:00 [medline]
PHST- 2021/04/25 20:55 [entrez]
AID - 10.1007/s10482-021-01580-0 [pii]
AID - 10.1007/s10482-021-01580-0 [doi]
PST - ppublish
SO  - Antonie Van Leeuwenhoek. 2021 Jul;114(7):1091-1106. doi: 
      10.1007/s10482-021-01580-0. Epub 2021 Apr 24.