PMID- 33896556
OWN - NLM
STAT- MEDLINE
DCOM- 20210514
LR  - 20210514
IS  - 1873-4324 (Electronic)
IS  - 0003-2670 (Linking)
VI  - 1161
DP  - 2021 May 29
TI  - A silicon nitride ISFET based immunosensor for tumor necrosis factor-alpha 
      detection in saliva. A promising tool for heart failure monitoring.
PG  - 338468
LID - S0003-2670(21)00294-4 [pii]
LID - 10.1016/j.aca.2021.338468 [doi]
AB  - According to the European statistics, approximately 26 million patients worldwide 
      suffer from heart failure (HF), and this number seems to be steadily increasing. 
      Inflammation plays a central role in the development of HF, and the 
      pro-inflammatory cytokine Tumor necrosis factor-alpha (TNF-alpha) represents inflammation 
      gold-standard biomarker. Early detection plays a crucial role for the prognosis 
      and treatment of HF. An Ion Sensitive Field Effect Transistor (ISFET) based on 
      silicon nitride transducer and biofunctionalized with anti-TNF-alpha antibody for 
      label-free detection of salivary TNF-alpha is proposed. Electrochemical impedance 
      spectroscopy (EIS) was used for TNF-alpha detection. Our ImmunoFET offered a 
      detection limit of 1 pg mL(-1), with an analytical reproducibility expressed by a 
      coefficient of variance (CV) resulted < 10% for the analysis of saliva samples, 
      and an analyte recovery of 94 +/- 6%. In addition, it demonstrated high selectivity 
      when compared to other HF biomarkers such as Inteleukin-10, N-terminal pro B-type 
      natriuretic peptide, and Cortisol. Finally, ImmunoFET accuracy in determining the 
      unknown concentration of TNF-alpha was successfully tested in saliva samples by 
      performing standard addition method. The proposed ImmunoFET showed great promise 
      as a complementary tool for biomedical application for HF monitoring by a 
      non-invasive, rapid and accurate assessment of TNF-alpha.
CI  - Copyright (c) 2021. Published by Elsevier B.V.
FAU - Ben Halima, Hamdi
AU  - Ben Halima H
AD  - Institute of Analytical Sciences (ISA) - UMR 5280, Claude Bernard Lyon 1 
      University, 69100, Villeurbanne, Lyon, France.
FAU - Bellagambi, Francesca G
AU  - Bellagambi FG
AD  - Institute of Analytical Sciences (ISA) - UMR 5280, Claude Bernard Lyon 1 
      University, 69100, Villeurbanne, Lyon, France. Electronic address: 
      francesca.bellagambi@univ-lyon1.fr.
FAU - Alcacer, Albert
AU  - Alcacer A
AD  - Institute of Microelectronics of Barcelona (IMB-CNM, CSIC), Campus UAB, 08193, 
      Bellaterra, Barcelona, Spain.
FAU - Pfeiffer, Norman
AU  - Pfeiffer N
AD  - Fraunhofer IIS, Fraunhofer Institute for Integrated Circuits, Am Wolfsmantel 33, 
      91058, Erlangen, Germany.
FAU - Heuberger, Albert
AU  - Heuberger A
AD  - Information Technology (LIKE), Friedrich-Alexander-Universitat Erlangen-Nurnberg 
      (FAU), Am Wolfsmantel 33, 91058, Erlangen, Germany.
FAU - Hangouet, Marie
AU  - Hangouet M
AD  - Institute of Analytical Sciences (ISA) - UMR 5280, French National Centre for 
      Scientific Research (CNRS), 69100, Villeurbanne, Lyon, France.
FAU - Zine, Nadia
AU  - Zine N
AD  - Institute of Analytical Sciences (ISA) - UMR 5280, Claude Bernard Lyon 1 
      University, 69100, Villeurbanne, Lyon, France.
FAU - Bausells, Joan
AU  - Bausells J
AD  - Institute of Microelectronics of Barcelona (IMB-CNM, CSIC), Campus UAB, 08193, 
      Bellaterra, Barcelona, Spain.
FAU - Elaissari, Abdelhamid
AU  - Elaissari A
AD  - Institute of Analytical Sciences (ISA) - UMR 5280, Claude Bernard Lyon 1 
      University, 69100, Villeurbanne, Lyon, France.
FAU - Errachid, Abdelhamid
AU  - Errachid A
AD  - Institute of Analytical Sciences (ISA) - UMR 5280, Claude Bernard Lyon 1 
      University, 69100, Villeurbanne, Lyon, France. Electronic address: 
      abdelhamid.errachid-el-salhi@univ-lyon1.fr.
LA  - eng
PT  - Journal Article
DEP - 20210402
PL  - Netherlands
TA  - Anal Chim Acta
JT  - Analytica chimica acta
JID - 0370534
RN  - 0 (Silicon Compounds)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - QHB8T06IDK (silicon nitride)
SB  - IM
MH  - *Biosensing Techniques
MH  - *Heart Failure/diagnosis
MH  - Humans
MH  - Immunoassay
MH  - Reproducibility of Results
MH  - Saliva
MH  - Silicon Compounds
MH  - Tumor Necrosis Factor-alpha
OTO - NOTNLM
OT  - Biosensor
OT  - Electrochemical impedance spectroscopy
OT  - Heart failure
OT  - ImmunoFET
OT  - Saliva analysis
OT  - Tumor necrosis factor-alpha
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2021/04/27 06:00
MHDA- 2021/05/15 06:00
CRDT- 2021/04/26 05:32
PHST- 2021/01/15 00:00 [received]
PHST- 2021/03/15 00:00 [revised]
PHST- 2021/03/29 00:00 [accepted]
PHST- 2021/04/26 05:32 [entrez]
PHST- 2021/04/27 06:00 [pubmed]
PHST- 2021/05/15 06:00 [medline]
AID - S0003-2670(21)00294-4 [pii]
AID - 10.1016/j.aca.2021.338468 [doi]
PST - ppublish
SO  - Anal Chim Acta. 2021 May 29;1161:338468. doi: 10.1016/j.aca.2021.338468. Epub 
      2021 Apr 2.