PMID- 33897690
OWN - NLM
STAT- MEDLINE
DCOM- 20210923
LR  - 20240401
IS  - 1664-3224 (Electronic)
IS  - 1664-3224 (Linking)
VI  - 12
DP  - 2021
TI  - The First Contact of Human Dendritic Cells With Trypanosoma cruzi Reveals 
      Response to Virus as an Unexplored Central Pathway.
PG  - 638020
LID - 10.3389/fimmu.2021.638020 [doi]
LID - 638020
AB  - Chagas disease is a debilitating and neglected disease caused by the protozoan 
      Trypanosoma cruzi. Soon after infection, interactions among T. cruzi and host 
      innate immunity cells can drive/contribute to disease outcome. Dendritic cells 
      (DCs), present in all tissues, are one of the first immune cells to interact with 
      Trypanosoma cruzi metacyclic trypomastigotes. Elucidating the immunological 
      events triggered immediately after parasite-human DCs encounter may aid in 
      understanding the role of DCs in the establishment of infection and in the course 
      of the disease. Therefore, we performed a transcriptomic analysis of a 12 h 
      interaction between T. cruzi and MoDCs (monocyte-derived DCs) from three human 
      donors. Enrichment analyses of the 468 differentially expressed genes (DEGs) 
      revealed viral infection response as the most regulated pathway. Additionally, 
      exogenous antigen processing and presentation through MHC-I, chemokine signaling, 
      lymphocyte co-stimulation, metallothioneins, and inflammasome activation were 
      found up-regulated. Notable, we were able to identify the increased gene 
      expression of alternative inflammasome sensors such as AIM2, IFI16, and RIG-I for 
      the first time in a T. cruzi infection. Both transcript and protein expression 
      levels suggest proinflammatory cytokine production during early T. cruzi-DCs 
      contact. Our transcriptome data unveil antiviral pathways as an unexplored 
      process during T. cruzi-DC initial interaction, disclosing a new panorama for the 
      study of Chagas disease outcomes.
CI  - Copyright (c) 2021 Gil-Jaramillo, Rocha, Raiol, Motta, Favali, Brigido, Bastos and 
      Santana.
FAU - Gil-Jaramillo, Natalia
AU  - Gil-Jaramillo N
AD  - Laboratorio de Interacao Patogeno-Hospedeiro, Instituto de Ciencias Biologicas, 
      Universidade de Brasilia, Brasilia, Brazil.
FAU - Rocha, Amanda Pereira
AU  - Rocha AP
AD  - Laboratorio de Interacao Patogeno-Hospedeiro, Instituto de Ciencias Biologicas, 
      Universidade de Brasilia, Brasilia, Brazil.
FAU - Raiol, Taina
AU  - Raiol T
AD  - Fiocruz Brasilia-Gerencia Regional de Brasilia (GEREB), Fundacao Oswaldo Cruz 
      (Fiocruz), Brasilia, Brazil.
FAU - Motta, Flavia Nader
AU  - Motta FN
AD  - Laboratorio de Interacao Patogeno-Hospedeiro, Instituto de Ciencias Biologicas, 
      Universidade de Brasilia, Brasilia, Brazil.
AD  - Faculdade de Ceilandia, Universidade de Brasilia, Brasilia, Brazil.
FAU - Favali, Cecilia
AU  - Favali C
AD  - Laboratorio de Imunologia Celular, Instituto de Ciencias Biologicas, Universidade 
      de Brasilia, Brasilia, Brazil.
FAU - Brigido, Marcelo M
AU  - Brigido MM
AD  - Laboratorio de Imunologia Molecular, Instituto de Ciencias Biologicas, 
      Universidade de Brasilia, Brasilia, Brazil.
FAU - Bastos, Izabela M D
AU  - Bastos IMD
AD  - Laboratorio de Interacao Patogeno-Hospedeiro, Instituto de Ciencias Biologicas, 
      Universidade de Brasilia, Brasilia, Brazil.
FAU - Santana, Jaime M
AU  - Santana JM
AD  - Laboratorio de Interacao Patogeno-Hospedeiro, Instituto de Ciencias Biologicas, 
      Universidade de Brasilia, Brasilia, Brazil.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210409
PL  - Switzerland
TA  - Front Immunol
JT  - Frontiers in immunology
JID - 101560960
RN  - 0 (AIM2 protein, human)
RN  - 0 (Cytokines)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Phosphoproteins)
RN  - 0 (Receptors, Immunologic)
RN  - 148998-64-5 (IFI16 protein, human)
RN  - EC 3.6.1.- (RIGI protein, human)
RN  - EC 3.6.4.13 (DEAD Box Protein 58)
SB  - IM
MH  - Adult
MH  - Antigen Presentation/immunology
MH  - Chagas Disease/*immunology
MH  - Cytokines/metabolism
MH  - DEAD Box Protein 58/metabolism
MH  - DNA-Binding Proteins/metabolism
MH  - Dendritic Cells/*immunology
MH  - Female
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation/genetics/immunology
MH  - Humans
MH  - Lymphocyte Activation/immunology
MH  - Male
MH  - Nuclear Proteins/metabolism
MH  - Phosphoproteins/metabolism
MH  - Receptors, Immunologic/metabolism
MH  - T-Lymphocytes/*immunology
MH  - Transcriptome/genetics
MH  - Trypanosoma cruzi/*immunology
MH  - Virus Diseases/*immunology
MH  - Young Adult
PMC - PMC8062726
OTO - NOTNLM
OT  - Chagas disease
OT  - inflammasome
OT  - metacyclic trypomastigotes
OT  - transcriptome
OT  - virus infection
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2021/04/27 06:00
MHDA- 2021/09/24 06:00
PMCR- 2021/01/01
CRDT- 2021/04/26 05:49
PHST- 2020/12/04 00:00 [received]
PHST- 2021/03/11 00:00 [accepted]
PHST- 2021/04/26 05:49 [entrez]
PHST- 2021/04/27 06:00 [pubmed]
PHST- 2021/09/24 06:00 [medline]
PHST- 2021/01/01 00:00 [pmc-release]
AID - 10.3389/fimmu.2021.638020 [doi]
PST - epublish
SO  - Front Immunol. 2021 Apr 9;12:638020. doi: 10.3389/fimmu.2021.638020. eCollection 
      2021.