PMID- 33899160
OWN - NLM
STAT- MEDLINE
DCOM- 20211105
LR  - 20211105
IS  - 1749-0774 (Electronic)
IS  - 0914-7470 (Linking)
VI  - 34
IP  - 4
DP  - 2021 Jul
TI  - Nisin and non-essential amino acids: new perspective in differentiation of neural 
      progenitors from human-induced pluripotent stem cells in vitro.
PG  - 1142-1152
LID - 10.1007/s13577-021-00537-9 [doi]
AB  - Over the past decades, stem cell therapy has been investigated as a promising 
      approach towards various diseases, including neurodegenerative disorders. Stem 
      cells show the capability to differentiate into neuronal progenitor cells in 
      vitro. In the present study, the differentiation potential of human-induced 
      pluripotent stem cells (hiPSCs) into neural lineages was examined under the 
      efficient induction media containing forskolin and 3-isobutyl-1-methyl-xanthine 
      (IBMX) in the presence of nisin (Ni), non-essential amino acids (NEAA) and 
      combination of those (NEAA-Ni) in vitro. The optimum concentrations of these 
      factors were obtained by MTT assay and acridine orange (AO) staining. The effect 
      of Ni and NEAA on the expression rate of neural-specific markers including NSE, 
      MAP2, and ss-tubulin III was studied via immunocytochemistry (ICC) and real-time 
      RT-PCR analyses. Our results indicated that the induction medium containing Ni or 
      NEAA increased the gene and protein expression of NSE, MAP2, and beta-tubulin III on 
      the 14th differentiation day. On the other hand, NEAA-Ni showed a 
      less-differentiated hiPSCs compared to Ni and NEAA alone. In conclusion, the 
      obtained results illustrated that Ni and NEAA could be applied as effective 
      factors for neural differentiation of hiPSCs in the future.
FAU - Eftekhari, Elahe
AU  - Eftekhari E
AD  - Department of Biology, Science and Research Branch, Islamic Azad University, 
      Tehran, Iran.
FAU - Ghollasi, Marzieh
AU  - Ghollasi M
AD  - Department of Cell and Molecular Biology, Faculty of Biological Sciences, 
      Kharazmi University, Tehran, Iran. ghollasi@khu.ac.ir.
FAU - Halabian, Raheleh
AU  - Halabian R
AD  - Applied Microbiology Research Center, Systems Biology and Poisonings Institute, 
      Baqiyatallah University of Medical Sciences, Tehran, Iran.
FAU - Soltanyzadeh, Maryam
AU  - Soltanyzadeh M
AD  - Department of Cell and Molecular Biology, Faculty of Biological Sciences, 
      Kharazmi University, Tehran, Iran.
FAU - Enderami, Seyed Ehsan
AU  - Enderami SE
AD  - Immunogenetics Research Center, Department of Medical Biotechnology, Faculty of 
      Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
LA  - eng
PT  - Journal Article
DEP - 20210425
PL  - Japan
TA  - Hum Cell
JT  - Human cell
JID - 8912329
RN  - 0 (Amino Acids)
RN  - 0 (Culture Media)
RN  - 0 (MAP2 protein, human)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (Tubulin)
RN  - 1414-45-5 (Nisin)
RN  - EC 4.2.1.11 (Phosphopyruvate Hydratase)
RN  - TBT296U68M (1-Methyl-3-isobutylxanthine)
SB  - IM
MH  - 1-Methyl-3-isobutylxanthine/pharmacology
MH  - Amino Acids/*pharmacology
MH  - Cell Differentiation/*drug effects/genetics
MH  - Cell- and Tissue-Based Therapy/methods
MH  - Cells, Cultured
MH  - Culture Media/chemistry
MH  - Gene Expression/drug effects
MH  - Humans
MH  - Induced Pluripotent Stem Cells/*physiology
MH  - Microtubule-Associated Proteins/genetics/metabolism
MH  - Neural Stem Cells/*physiology
MH  - Neurodegenerative Diseases/therapy
MH  - Neurons/*physiology
MH  - Nisin/*pharmacology
MH  - Phosphopyruvate Hydratase/genetics/metabolism
MH  - Tubulin/genetics/metabolism
OTO - NOTNLM
OT  - Induced pluripotent stem cells
OT  - Nisin
OT  - Non-essential amino acids
EDAT- 2021/04/27 06:00
MHDA- 2021/11/06 06:00
CRDT- 2021/04/26 06:11
PHST- 2020/12/27 00:00 [received]
PHST- 2021/04/16 00:00 [accepted]
PHST- 2021/04/27 06:00 [pubmed]
PHST- 2021/11/06 06:00 [medline]
PHST- 2021/04/26 06:11 [entrez]
AID - 10.1007/s13577-021-00537-9 [pii]
AID - 10.1007/s13577-021-00537-9 [doi]
PST - ppublish
SO  - Hum Cell. 2021 Jul;34(4):1142-1152. doi: 10.1007/s13577-021-00537-9. Epub 2021 
      Apr 25.